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The population genetics and origin of invasion of the invasive Asian paddle crab, Charybdis japonica (A. Milne-Edwards, 1861) (Brachyura: Portunidae) in north-eastern New Zealand

Marine Biology volume 163, Article number: 133 (2016) Cite this article

Abstract

Charybdis japonica is a recently established invasive crab in north-eastern New Zealand that is native to East Asia. Since its detection in 2000, C. japonica has spread to adjacent estuaries from the introduction site at the Ports of Auckland and established 127 km north in Whangarei Harbour. We assessed the invasion history from comparisons of genetic population diversity and mixed-stock analysis from the mitochondrial control region and nuclear ribosomal internal transcribed spacer-1, to describe the invasive population’s genetic structure, infer a possible source of origin and hypothesize the composition of the founding colonists. High diversity and distinct genetic structuring were found within and among native localities from Japan, China, Taiwan and South Korea. Crabs collected at New Zealand locations (Auckland, Whangaparaoa and Whangarei) had lower diversity and were genetically homogenous. This indicated C. japonica experienced a bottleneck on colonization, but the resultant founder effects have not prevented C. japonica from establishing and spreading along New Zealand’s north-eastern coast. Among the sampled native localities, mixed-stock and multidimensional scaling analyses revealed Japan as the most likely source of the New Zealand invasion. The presence of three mitochondrial haplotypes and five ITS-1 alleles identified from the earliest samples in 2002 suggested that the founding New Zealand population descended from as few as three individuals. Evidence also suggests the occurrence of a second invasion event into Whangarei, New Zealand. Our study has provided the baseline for a more precise examination of Japan as the source of the invasive C. japonica population in New Zealand and suggests the importance of this recurring invasion pathway.

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Acknowledgments

Funding for research was provided by the University of Auckland. Thanks to the management of the Weiti River Boat Club, Westhaven Marina, Halfmoon Bay Marina and Westpark Marina for permission to collect the Auckland samples. We are very grateful to the numerous people who contributed time and effort to the collection of potential source and non-Auckland samples, including T.Y. Chan (National Taiwan Ocean University), M.V. Archdale (Kagoshima University), S. Ahyong (Australia Museum), S.K. Lee (Seoul National University) and G. Townsend (Northland Regional Council, Whangarei). Thanks to the reviewers whose suggestions have significantly improved the manuscript.

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  1. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand

    N. A. Wong, L. K. Tooman, M. A. Sewell & S. D. Lavery

  2. Center for Applied Neurogenetics, University of British Columbia, Vancouver, V6T 2B5, Canada

    L. K. Tooman

  3. Institute of Marine Science, University of Auckland, Private Bag 92019, Auckland, 1001, New Zealand

    S. D. Lavery

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  1. N. A. Wong
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Wong, N.A., Tooman, L.K., Sewell, M.A. et al. The population genetics and origin of invasion of the invasive Asian paddle crab, Charybdis japonica (A. Milne-Edwards, 1861) (Brachyura: Portunidae) in north-eastern New Zealand. Mar Biol 163, 133 (2016). https://doi.org/10.1007/s00227-016-2906-y

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Keywords

  • Native Locality
  • Genetic Bottleneck
  • Invasive Population
  • Mitochondrial Control Region
  • Zealand Population