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Physiological and photophysiological responses of the benthic diatom Entomoneis paludosa (Bacillariophyceae) to dissolved inorganic and organic nitrogen in culture

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Abstract

Benthic diatoms are dominant primary producers in intertidal flats. This study investigated the effect of different nitrogen sources and concentrations on Entomoneis paludosa growth and physiological responses. Six nitrogen sources either inorganic (nitrate and ammonium) or organic (urea, arginine, glutamine and glycine) supplied at two concentrations (40 and 400 µM-N) induced significant effects on growth, carbon, nitrogen, pigment content and maximum PSII quantum efficiency (F v /F m). Entomoneis paludosa grew under all nitrogen sources albeit showing differences in lag phase, growth rate and cell yield. Inorganic nitrogen, urea and arginine induced higher growth, whereas glycine did not support high biomass. F v /F m showed variability dependent on nitrogen source and C/N ratio. F v /F m varied between 0.55 and 0.65 at 400 μM-N with the highest values observed in glycine, glutamine and urea, whereas nitrate, ammonium and arginine induced lower F v /F m. All cellular components decreased in the 40 µM-N treatments, with nitrogen and pigments being lower than carbon content. Light-harvesting pigment ratios Chl c/Chl a and photoprotective pigment ratios (diatoxanthin + diadinoxanthin)/Chl a increased, while fucoxanthin/Chl a ratios were unaffected by N-limitations. Entomoneis paludosa was capable of quickly adapting and using a wide variety of nitrogen sources. This adaptability may contribute to microphytobenthos diatom ecological success in mudflat ecosystems.

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Acknowledgments

This study was part of the COSELMAR project carried out with the support of the “Region Pays de la Loire”, the “MSH Ange Guépin”, the Research Federation CNRS 3473 “Institut Universitaire Mer et Littoral” and the University of Nantes. The author would also like to Denis Loquet from the CEISAM for the elemental analysis.

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Author notes

  1. Thierry Jauffrais

    Present address: Laboratoire des Bio-Indicateurs Actuels et Fossiles, UMR CNRS 6112 LPG-BIAF, Université d’Angers, 49045, Angers Cedex 1, France

Authors and Affiliations

  1. Laboratoire Mer Molécules Santé EA2160, FR CNRS 3473, Faculté des Sciences et des Techniques, Université de Nantes, 44322, Nantes Cedex 3, France

    Thierry Jauffrais, Bruno Jesus, Vona Méléder, Vincent Turpin & Véronique Martin Jézéquel

  2. BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisboa, Campo Grande, 1749-016, Lisbon, Portugal

    Bruno Jesus

  3. Instituto de Oceanografia, Universidade Federal do Rio Grande (FURG), Av. Itália, km 8, Rio Grande, RS, 96201-900, Brazil

    Arnaldo D`Amaral P. G. Russo

  4. Université d’Aix Marseille, CNRS INSU, IRD, MIO, UM 110, Marseille, France

    Patrick Raimbault

  5. Institut Littoral Environnement et Sociétés LIENSs, UMR CNRS 7266, Université de La Rochelle, La Rochelle, France

    Véronique Martin Jézéquel

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Correspondence to Thierry Jauffrais.

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Jauffrais, T., Jesus, B., Méléder, V. et al. Physiological and photophysiological responses of the benthic diatom Entomoneis paludosa (Bacillariophyceae) to dissolved inorganic and organic nitrogen in culture. Mar Biol 163, 115 (2016). https://doi.org/10.1007/s00227-016-2888-9

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