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Geographic variation in allele frequency of the gamete recognition protein M7 lysin throughout a mosaic blue mussel hybrid zone

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Abstract

Divergence at gamete recognition loci is hypothesized to result in speciation in broadcast spawning invertebrates. Many gamete recognition loci evolve quickly and show patterns of positive selection, yet the advantage of divergence is rarely known. M7 lysin is a sperm protein in the Mytilus edulis species complex that shows evidence of adaptive evolution. This locus is polymorphic with two distinct clades within Mytilus galloprovincialis, one of which, the D clade, shows the strongest signal of positive selection. We tested whether the geographic patterns in allele frequency were consistent with the hypothesis that positive selection on D clade alleles (G D ) was due to reinforcement. Populations of M. edulis showed little evidence of introgression of G D alleles, but there was no consistent evidence of reproductive character displacement. The lack of consistent patterns expected of reinforcement suggests that another mechanism is likely responsible for the adaptive divergence of M7 lysin.

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Acknowledgments

We thank R.R. Kirby for valuable discussions of the results and for providing laboratory space at the Marine Biological Association in Plymouth, UK, and also Rhiannon Rognstad for mapping input. Funding for this project was provided by the National Science Foundation grant number DEB-0818661 and a UNF Undergraduate Summer Research Award.

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Authors and Affiliations

  1. Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA

    Matthew R. Gilg, M. Camila Restrepo, Ronald Walton & Emmanuel Rodriguez

  2. Department of Biological Sciences, Auburn University, Auburn, AL, 36849, USA

    Pamela M. Brannock

  3. Department of Biology, University of South Carolina, Columbia, SC, 29208, USA

    Thomas J. Hilbish

Authors
  1. Matthew R. Gilg
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  2. M. Camila Restrepo
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  3. Ronald Walton
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  4. Pamela M. Brannock
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  5. Thomas J. Hilbish
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  6. Emmanuel Rodriguez
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Correspondence to Matthew R. Gilg.

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Communicated by T. Reusch.

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Gilg, M.R., Camila Restrepo, M., Walton, R. et al. Geographic variation in allele frequency of the gamete recognition protein M7 lysin throughout a mosaic blue mussel hybrid zone. Mar Biol 160, 1737–1750 (2013). https://doi.org/10.1007/s00227-013-2226-4

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  • DOI: https://doi.org/10.1007/s00227-013-2226-4

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