Abstract
This study aims at establishing the maturation of the digestive system in Robsonella fontaniana by means of measuring the digestive enzyme activity in paralarvae after hatching. Different groups of newly hatched paralarvae were fed with Artemia sp. (FA), Lithodes santolla zoea (FL) or were starved to serve as negative control (ST), the experimental period lasted 27 days after hatching (DAH). The semi-quantitative assay api® ZYM (bioMérieux, France) was used to identify the pool of digestive enzymes present in paralarvae prior to selecting the specific enzymes acid phosphatase, total protease, trypsin and chymotrypsin for analysis. Mortality and larval protein content were also measured during the experiment. The survival of R. fontaniana hatchlings differed depending on the quality of the diet offered (ST < FA < FL), the same trend was observed with respect to paralarval protein content. Total protease and acid phosphatase activities were not related to either time of development or diet (P < 0.05). However, the activity of trypsin and chymotrypsin after DAH 10 was dependent on diet (P < 0.05). Paralarvae fed with Lithodes zoea (FL) performed better than paralarvae in the other two groups, exhibiting lower mortality, higher protein content and the highest proteolytic activity for trypsin and chymotrypsin. The results indicate that Lithodes-larvae are a better diet for R. fontaniana paralarvae than Artemia sp.
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Acknowledgments
Authors greatly appreciate the help granted by Dr. Kurt Paschke who provided the laboratory equipment needed to process and analyze the enzyme samples. Dr. Carlos Rosas also helped us to standardize enzyme assays used and Mr. Jorge Hernández who helped with the experimental setup. We also thank Dr. Robert A. Stead for reviewing the English, and also for critical reading of the MS. This study was funded by the Fondef D04I1401 granted to I. Uriarte. The experiments comply with the current laws in Chile.
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Communicated by H. O. Pörtner.
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Pereda, S.V., Uriarte, I. & Cabrera, J.C. Effect of diet and paralarval development on digestive enzyme activity in the cephalopod Robsonella fontaniana . Mar Biol 156, 2121–2128 (2009). https://doi.org/10.1007/s00227-009-1242-x
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DOI: https://doi.org/10.1007/s00227-009-1242-x