Recovery of novel bacterial diversity from mangrove sediment
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Bacteria in the surface sediments of a subtropical mangrove habitat were investigated using a cultivation-independent molecular approach. Phylogenetic analysis of nearly full-length 16S rRNA genes revealed a diversity of sequences that were mostly novel. Members from the five subdivisions of the Proteobacteria were detected, and they collectively represented the majority (67%) of the clone library. Sequence types affiliated with the Gammaproteobacteria constituted the largest portion (29%) of the library, and many of them were related to free-living and symbiotic sulfur-oxidizing bacteria. The Epsilonproteobacteria were the second most abundant group (16%), including only one sequence type clustering with PCR-generated bacterial clones previously recovered from deep-sea sediments. A substantial portion (8%) of the clones grouped within the Deltaproteobacteria, a subdivision with anaerobic sulfate or metal reduction as the predominant metabolic trait of its members. In addition, minor portions were affiliated with the Cytophaga–Flexibacter–Bacteroides group (9%), Actinobacteria (6%), Chloroflexi (5%), Firmicutes (4%), Fusobacteria (1%), and the Chlamydiae/Verrucomicrobia group, Fibrobacteres/Acidobacteria group and Planctomycetes (each < 1%). These results significantly expand our knowledge of the bacterial diversity of the unique mangrove environment.
KeywordsrRNA Gene Sequence Clone Library Sequence Type Terminal Restriction Fragment Length Polymorphism Mangrove Ecosystem
We would like to thank the Futian-City University Mangrove Research & Development Center for their support in conducting field sampling. The work described in this paper was supported by a grant from the City University of Hong Kong (Project No. 7001690).
- Dilling W, Liesack W, Pfennig N (1995) Rhabdochromatium marinum gen. nom. rev., sp. nov., a purple sulfur bacterium from a salt marsh microbial mat. Arch Microbiol 164:125–131Google Scholar
- Kristensen E, King GM, Banta GT, Holmer M, Jensen MH, Hansen K, Bussarawit N (1994) Sulfate reduction, acetate turnover and carbon metabolism in sediments of the Ao-Nam-Bor mangrove, Phuket, Thailand. Mar Ecol Prog Ser 109:245–255Google Scholar
- Kristensen E, Andersen FØ, Holmboe N, Holmer M, Thongtham N (2000) Carbon and nitrogen mineralization in sediments of the Bangrong mangrove area, Phuket, Thailand. Aquat Microb Ecol 22:199–213Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, NY, pp 115–175Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Swofford DL (1999) PAUP: phylogenetic analysis using parsimony, version 4.0. Illinois Natural History Survey, Champaign, ILGoogle Scholar