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Inter-simple sequence repeat markers reveal strong genetic differentiation among populations of the endangered mollusc Patella ferruginea (Gastropoda: Patellidae) from two Sardinian marine protected areas

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Abstract

The giant Mediterranean limpet Patella ferruginea Gmelin, 1791 is an endangered marine gastropod, whose range has progressively shrinked to few, restricted areas, due to intense human exploitation. We have studied the genetic structure of the species, in order to (1) gather information about the levels of genetic variability within and between natural populations of P. ferruginea collected in two Sardinian marine protected areas (MPAs) (Penisola del Sinis—Isola di Mal di Ventre and Isola dell’ Asinara), and (2) make an attempt to find relationships between ecological and biological attributes of the species and the genetic differentiation of the populations studied. The genetic study was carried out by means of the analysis of ten inter-simple sequence repeat (ISSR) primers on a total of 40 individuals collected at four localities. Genetic analysis evidenced (1) medium to high levels of within-population genetic variability, (2) a pattern of genetic structuring that varied with spatial scales, and (3) a strong genetic differentiation between the two MPAs. Although preliminary, these results suggest that gene flow may be present only at very small geographic scale, raising concerns on the future of the conservation of the species.

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Acknowledgements

This research was financially supported by the European Community INTERREG III and by the “Centro di Eccellenza” of the University of Sassari, Italy. We gratefully acknowledge David Pala, for invaluable help during field work. Thanks also at the whole staff of the Isola dell’ Asinara and Penisola del Sinis-Isola di Mal di Ventre MPAs for support and assistance. We also wish to thank Valentina Bacciu, Antonio Cossu, Pierpaolo Mossa, and Giuseppe Tanda for their help during laboratory work.

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Authors and Affiliations

  1. Dipartimento di Zoologia e Antropologia Biologica, Universitá di Sassari, Via F. Muroni 25, 07100, Sassari, Italy

    Marco Casu, Daniela Casu, Tiziana Lai, Piero Cossu & Marco Curini-Galletti

  2. Dipartimento di Scienze dell‘Uomo e dell’Ambiente, Università di Pisa, Via A. Volta 6, 56126, Pisa, Italy

    Daniela Casu

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  1. Marco Casu
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Correspondence to Marco Casu.

Additional information

Communicated by R. Cattaneo-Vietti, Genova

Appendix

Appendix

Table 5 P. ferruginea: ISSR primers, approximate band sizes (bp) and profiles obtained for 40 individuals from four localities. Sample-private bands are in bold
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Casu, M., Casu, D., Lai, T. et al. Inter-simple sequence repeat markers reveal strong genetic differentiation among populations of the endangered mollusc Patella ferruginea (Gastropoda: Patellidae) from two Sardinian marine protected areas. Mar Biol 149, 1163–1174 (2006). https://doi.org/10.1007/s00227-006-0255-y

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