Noncovalent bonds are key mechanisms for the cohesion of barnacle (Balanus crenatus) adhesive proteins
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Cement of the barnacle species, Balanus crenatus, was isolated and separated by SDS-PAGE. Reductive and nonreductive conditions yielded the same result of six major proteins (in the range up to 100 kDa) occurring as two triplets. A third triplet of lower molecular mass was faintly visible. The presence of a reductive agent had only little impact on the solubility of B. crenatus cement. The strong effect of the denaturing conditions (the presence of SDS and the incubation at elevated temperature) on the solubility of the barnacle cement and the absence of disulfide signals in Raman spectra suggest a complex structure of the barnacle cement noncovalently bound. It can be speculated that this protein complex in its quaternary structure is formed by small subunits and is equivalent to the globular cement structures previously detected.
KeywordsSodium Dodecyl Sulphate PDMS Nonreducing Condition Noncovalent Bond Amino Acid Cysteine
This work is part of a Ph.D. thesis, which was financially supported by the scholarship program of the German Federal Environmental Foundation and by the Fraunhofer Institute for Production Technology and Applied Materials Research—IFAM, Bremen. The support of the laboratory of general microbiology of Prof. Reinhold-Hurek (University Bremen) while conducting the purification and fractioning of the samples is greatly appreciated.
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