Marine Biology

, Volume 149, Issue 1, pp 49–55 | Cite as

Assessment of estrogenic chemicals using an estrogen receptor α (ERα)- and ERβ-mediated reporter gene assay in fish

  • T. KitanoEmail author
  • T. Koyanagi
  • R. Adachi
  • N. Sakimura
  • K. Takamune
  • S.-I. Abe
Research Article


In vitro reporter gene assays using vertebrate cell lines or yeast have been used for assessment of the estrogenic chemicals. However, estrogen receptor α (ERα)- and ERβ-mediated reporter gene system in fish has yet to be developed. In the present study, we developed an ERα- and ERβ-mediated reporter gene assay in fish and estimated estrogenic activities of 17β-estradiol (E2), p-nonylphenol (NP), bisphenol A (BPA), p,p′-DDE, and genistein (Gen) using the in vitro reporter assay. The activity was intensely induced by transfection with either ERα or ERβ expression plasmid under E2 or Gen administration, whereas it was significantly induced by transfection with ERα expression plasmid, but not with ERβ expression plasmid under NP administration. On the other hand, the activity was induced more intensely by transfection with ERα expression plasmid than ERβ expression plasmid under BPA or p,p′-DDE administration. These results indicate that there are obvious differences in the activity through ERα and ERβ among the estrogenic chemicals examined in vitro. Thus, the in vitro reporter assay provides an excellent system for elucidating the action mechanism of estrogenic chemicals in fishes.


Estrogen Receptor Genistein Reporter Gene Assay Estrogenic Chemical DDBJ Accession 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Professor Yoshitaka Nagahama for helpful advice. This work was supported by CREST (Core Research for Evolutional Science and Technology) of JST (Japan Science and Technology Corporation).


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • T. Kitano
    • 1
    Email author
  • T. Koyanagi
    • 2
  • R. Adachi
    • 2
  • N. Sakimura
    • 1
  • K. Takamune
    • 1
  • S.-I. Abe
    • 1
  1. 1.Faculty of ScienceKumamoto UniversityKumamotoJapan
  2. 2.Department of Materials and Life Science, Graduate School of Science and TechnologyKumamoto University KumamotoJapan

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