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RFLP analysis of the mtDNA control region in white shrimp (Litopenaeus vannamei) populations from the eastern Pacific

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Abstract

White shrimp (Litopenaeus vannamei) population genetic structure from the eastern Pacific was determined by restriction fragment length polymorphism analysis of the mitochondrial DNA control region. Four localities were surveyed with four endonucleases (Alu I, Taq I, Spe I, Ssp I) yielding 48 composite haplotypes. White shrimp showed high average within-locality haplotype (0.823) and nucleotide (5.41%) diversities and also high average nucleotide divergence between all pairs of localities (0.73%). A mismatch analysis of pairwise differences between haplotypes indicated that white shrimp does not fit the sudden population expansion model. An analysis of molecular variance showed significant geographic variation in the frequencies of haplotypes (ΦST=0.1382, P<0.0001). Population differentiation may be maintained by a combination of physical, oceanographic, and biological factors acting as barriers to gene flow among localities. Because of its high polymorphism, the control region might be useful as a genetic marker for monitoring genetic diversity in aquaculture stocks.

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Acknowledgements

Funding was provided by CONACYT (33496-V) and FOMIX Nayarit-CONACYT (2003-COI-9661) grants to R.P.E. The first author is a CONACYT graduate fellow (85938) and is grateful to the College of Marine Studies, University of Delaware, for the facilities provided during a research stay. Thanks to F. Garcia for his advice about the REAP program.

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Authors and Affiliations

  1. Aquaculture Genetics, Centro de Investigaciones Biológicas del Noroeste, S. C. (CIBNOR), A.P. 128, La Paz, Baja California Sur, México, 23000, Mexico

    R. Valles-Jimenez & R. Perez-Enriquez

  2. College of Marine Studies, University of Delaware, Lewes, DE, 19958, USA

    P. M. Gaffney

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  1. R. Valles-Jimenez
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  2. P. M. Gaffney
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  3. R. Perez-Enriquez
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Correspondence to R. Perez-Enriquez.

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Communicated by P. W. Sammarco, Chauvin

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Valles-Jimenez, R., Gaffney, P.M. & Perez-Enriquez, R. RFLP analysis of the mtDNA control region in white shrimp (Litopenaeus vannamei) populations from the eastern Pacific. Marine Biology 148, 867–873 (2006). https://doi.org/10.1007/s00227-005-0122-2

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