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The Arctic sea butterfly Limacina helicina: lipids and life strategy

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Abstract

The sea butterfly Limacina helicina was collected from May to September 2001 in Kongsfjorden, Spitsbergen, to investigate population structure and body and lipid composition with regard to life cycle and reproductive strategy. Veligers and juveniles were only found in late autumn and spring, whereas females occurred from July to September. The size of the females increased until mid-August and decreased in September. Dry and lipid mass were closely related to size; dry mass increased exponentially and lipids linearly with size. The lipid content was highest in veligers (31.5% of dry mass) and juveniles (23.6%) but low in females (<10%). Phospholipids were the dominating lipid class followed by triacylglycerols. Females, veligers, and egg ribbons, all from September, were richest in phospholipids. Juveniles contained the highest amounts of triacylglycerols, whereas females had low levels in July and the beginning of August. In mid-August, levels of triacylglycerols were variable and higher. This suggests that females were in their main spawning period and the high variability in triacylglycerols points to different stages within the spawning cycle. Enhanced amounts of free fatty acids in females from July may be related to gonad development. The 16:1(n-7) fatty acid was more dominant in spring whereas 18:4(n-3) increased in summer and autumn, which reflects a change in diet from diatom-dominated food items in spring to dinoflagellates in summer/autumn. Small amounts of long-chain monounsaturated fatty acids suggest ingestion of copepods, and the fatty acid composition of veligers feeding on particulate matter. L. helicina has a one-year life cycle with peak spawning in August and over-winters as veligers that may grow to juveniles during the winter period. They metamorphose into juveniles during spring, develop to males in early summer, and mature into females in July and August.

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References

  • Albers CS, Kattner G, Hagen W (1996) The compositions of wax esters, triacylglycerols and phospholipids in Arctic and Antarctic copepods: evidence of energetic adaptations. Mar Chem 55:347–358

    Article  CAS  Google Scholar 

  • Conover RJ, Huntley M (1991) Copepods in ice-covered seas. Distribution, adaptations to seasonally limited food, metabolism, growth patterns and life cycle strategies in polar seas. J Mar Syst 2:1–41

    Google Scholar 

  • Conover RJ, Lalli CM (1972) Feeding and growth in Clione limacina (Phipps), a pteropod mollusc. J Exp Mar Biol Ecol 9:279–302

    Google Scholar 

  • Cooney RT, Coyle KO, Stockmar E, Stark C (2001) Seasonality in surface-layer net zooplankton communities in Prince William Sound, Alaska. Fish Oceanogr 10:97–109

    Google Scholar 

  • Dalsgaard J, St. John M, Kattner G, Müller-Navarra D, Hagen W (2003) Fatty acid trophic markers in the pelagic marine environment. Adv Mar Biol 46:225–340

    Google Scholar 

  • Falk-Petersen S, Hopkins CCE, Sargent JR (1990) Trophic relationships in the pelagic arctic food web. In: Barnes M, Gibson RN (eds) Trophic relations in the marine environment. Aberdeen University Press, Aberdeen, pp 315–333

  • Falk-Petersen S, Pedersen G, Kwasniewski S, Hegseth EN, Hop H (1999) Spatial distribution and life-cycle timing of zooplankton in the marginal ice zone of the Barents Sea during the summer melt season in 1995. J Plankton Res 21:1249–1264

    Google Scholar 

  • Falk-Petersen S, Sargent JR, Kwasniewski S, Gulliksen B, Millar R-M (2001) Lipids and fatty acids in Clione limacina and Limacina helicina in Svalbard waters and the Arctic Ocean: trophic implications. Polar Biol 24:163–170

    Article  Google Scholar 

  • Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  PubMed  Google Scholar 

  • Gilmer RW (1972) Free-floating mucus webs—novel feeding adaptation for open ocean. Science 176:1239–1240

    Google Scholar 

  • Gilmer RW (1990) In situ observations of feeding behavior of thecosome pteropod molluscs. Am Malacol Bull 8:53–59

    Google Scholar 

  • Gilmer RW, Harbison GR (1991) Diet of Limacina helicina (Gastropoda: Thecosomata) in Arctic waters in midsummer. Mar Ecol Prog Ser 77:125–134

    Google Scholar 

  • Graeve M, Kattner G, Hagen W (1994) Diet-induced changes in the fatty acid composition of Arctic herbivorous copepods: experimental evidence of trophic markers. J Exp Mar Biol Ecol 182:97–110

    Google Scholar 

  • Hama T (1999) Fatty acid composition of particulate matter and photosynthetic products in subarctic and subtropical Pacific. J Plankton Res 21:1355–1372

    Google Scholar 

  • Hamner WM, Madin LP, Alldredge AL, Gilmer RW, Hamner PP (1975) Underwater observations of gelatinous zooplankton: sampling problems, feeding biology, and behavior. Limnol Oceanogr 20:907–917

    Google Scholar 

  • Harbison GR, Gilmer RW (1992) Swimming, buoyancy and feeding in shelled pteropods—a comparison of field and laboratory observations. J Mollusc Stud 58:337–339

    Google Scholar 

  • Hirche HJ, Niehoff B (1996) Reproduction of the Arctic copepod Calanus hyperboreus in the Greenland Sea—field and laboratory observations. Polar Biol 16:209–219

    Article  Google Scholar 

  • Hop H, Pearson T, Hegseth EN, Kovacs KM, Wiencke C, Kwasniewski S, Eiane K, Mehlum F, Gulliksen B, Wlodarska-Kowalczuk M, Lydersen C, Weslawski JM, Cochrane S, Gabrielsen GW, Leakey RJG, Lønne OJ, Zajaczkowski M, Falk-Petersen S, Kendall M, Wängberg S-A, Bischof K, Voronkov AY, Kovaltchouk NA, Wiktor J, Poltermann M, di Prisco G, Papucci C, Gerland S (2002) The marine ecosystem of Kongsfjorden, Svalbard. Polar Res 21:167–208

    Google Scholar 

  • Kattner G, Fricke HSG (1986) Simple gas-liquid chromatographic method for the simultaneous determination of fatty acids and alcohols in wax esters of marine organisms. J Chromatogr 361:263–268

    Google Scholar 

  • Kattner G, Hagen W (1995) Polar herbivorous copepods—different pathways in lipid biosynthesis. ICES J Mar Sci 52:329–335

    Article  Google Scholar 

  • Kattner G, Hagen W, Graeve M, Albers C (1998) Exceptional lipids and fatty acids in the pteropod Clione limacina (Gastropoda) from both polar oceans. Mar Chem 61:219–228

    Google Scholar 

  • Kerswill CJ (1940) The distribution of pteropods in the waters of eastern Canada and Newfoundland. J Fish Res Bd Can 5:23–31

    Google Scholar 

  • Kobayashi HA (1974) Growth-cycle and related vertical distribution of thecosomatous pteropod Spiratella (“Limacina”) helicina in central Arctic Ocean. Mar Biol 26:295–301

    Google Scholar 

  • Kwasniewski S, Hop H, Falk-Petersen S, Pedersen G (2003) Distribution of Calanus species in Kongsfjorden, a glacial fjord in Svalbard. J Plankton Res 25:1–20

    Google Scholar 

  • Lalli CM (1970) Structure and function of the buccal apparatus of Clione limacina (Phipps) with a review of feeding in gymnosomatous pteropods. J Exp Mar Biol Ecol 4:101–118

    Google Scholar 

  • Lalli CM, Gilmer RW (1989a) The thecosomes, shelled pteropods. In: Lalli CM, Gilmer RW (eds) Pelagic snails. The biology of holoplanktonic gastropod mollusks. Stanford University Press, Stanford, Calif., pp 58−166

  • Lalli CM, Gilmer RW (1989b) Pelagic snails. The biology of holoplanktonic gastropod mollusks. Stanford University Press, Stanford, Calif.

  • Lalli CM, Wells FE (1978) Reproduction in genus Limacina (Opisthobranchia: Thecosomata). J Zool 186:95–108

    Google Scholar 

  • Larson RJ, Harbison GR (1989) Source and fate of lipids in polar gelatinous zooplankton. Arctic 42:339–346

    Google Scholar 

  • Lee RF (1974) Lipid composition of the copepod Calanus hyperboreus from the Arctic Ocean. Changes with depth and season. Mar Biol 26:313–318

    Google Scholar 

  • Lee RF (1975) Lipids of arctic zooplankton. Comp Biochem Physiol 51:263–266

    Google Scholar 

  • Lee RF (1991) Lipoproteins from the hemolymph and ovaries of marine invertebrates. In: Gilles R (ed) Advances in comparative and environmental physiology, vol 7. Springer, Berlin Heidelberg New York, 187–207

  • Meisenheimer J (1905) Die arktischen Pteropoden. Fauna Arctica 4:410–430

    Google Scholar 

  • Olsen RE, Henderson RJ (1989) The rapid analysis of neutral and polar marine lipids using double-development HPTLC and scanning densitometry. J Exp Mar Biol Ecol 129:189–197

    Google Scholar 

  • Omori M, Hamner WM (1982) Patchy distribution of zooplankton—behavior, population assessment and sampling problems. Mar Biol 72:193–200

    Google Scholar 

  • Paranjape MA (1968) The egg mass and veliger of Limacina helicina Phipps. Veliger 10:322–326

    Google Scholar 

  • Percy JA, Fife FJ (1981) The biochemical composition and energy content of arctic marine macrozooplankton. Arctic 34:307–313

    Google Scholar 

  • Percy JA, Fife FJ (1985) Energy distribution in an Arctic coastal macrozooplankton community. Arctic 38:39–42

    Google Scholar 

  • Phleger CF, Nichols PD, Virtue P (1997) Lipids and buoyancy in Southern Ocean pteropods. Lipids 32:1093–1100

    Google Scholar 

  • Sakshaug E (2003) Primary and secondary production in the Arctic seas. In: Stein R, Macdonald RW (eds) The organic carbon cycle in the Arctic Ocean. Springer, Berlin Heidelberg New York, pp 57–81

  • Scott CL, Kwasniewski S, Falk-Petersen S, Sargent JR (2002a) Lipids and fatty acids in the copepod Jaschnovia brevis (Jaschnov) and in particulates from Arctic waters. Polar Biol 25:65–71

    Google Scholar 

  • Scott CL, Kwasniewski S, Falk-Petersen S, Sargent JR (2002b) Species differences, origins and functions of fatty alcohols and fatty acids in the wax esters and phospholipids of Calanus hyperboreus, C. glacialis and C. finmarchicus from Arctic waters. Mar Ecol Prog Ser 235:127–134

    Google Scholar 

  • Spoel S van der, Dadon JR (1999) Pteropoda. In: Boltovskoy D (ed) South Atlantic zooplankton. Backhhuys, Leiden, The Netherlands, pp 649–706

  • Svendsen H, Beszczynska-Møller A, Hagen JO, Lefauconnier B, Tverberg V, Gerland S, Ørbaek JB, Bischof K, Papucci C, Zajaczkowski M, Azzolini R, Bruland O, Wiencke C, Winther J-G, Dallmann W (2002) The physical environment of Kongsfjorden-Krossfjorden, an Arctic fjord system in Svalbard. Polar Res 21:133–166

    Google Scholar 

  • Weslawski JM, Pedersen G, Falk-Petersen S, Porazinski K (2000) Entrapment of macroplankton in an Arctic fjord basin, Kongsfjorden, Svalbard. Oceanologia 42:57–69

    Google Scholar 

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Acknowledgements

We thank the captain and crew of “R.V. Lance”, “R.V. Håkon Mosby”, and “R.V. Oceania”, the staff at Kings Bay Research Facility for their professional support, and Audun Igesund for providing the map. The work was supported by the Personnel Exchange Programme between the Norwegian Research Council and Deutscher Akademischer Austauschdienst (DAAD).

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Correspondence to Stig Falk-Petersen.

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Communicated by M. Kühl, Helsingør

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Gannefors, C., Böer, M., Kattner, G. et al. The Arctic sea butterfly Limacina helicina: lipids and life strategy. Marine Biology 147, 169–177 (2005). https://doi.org/10.1007/s00227-004-1544-y

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