Wood Science and Technology

, Volume 47, Issue 3, pp 523–535 | Cite as

MALDI-TOF, HPLC-ESI-TOF and 13C-NMR characterization of chestnut (Castanea sativa) shell tannins for wood adhesives

  • Gonzalo Vázquez
  • Antonio Pizzi
  • M. Sonia Freire
  • Jorge Santos
  • Gervasio Antorrena
  • Julia González-ÁlvarezEmail author


MALDI-TOF, HPLC-ESI-TOF and 13C-NMR techniques were used to analyse the structure of non-purified aqueous chestnut shell tannin extracts. In addition, the influence of the extraction agent (water or aqueous solutions of Na2SO3 and/or NaOH) on tannin structure was analysed by MALDI-TOF in order to select the extract with the best properties for wood adhesives. Using HPLC-ESI-TOF, catechin/epicatechin, gallocatechin/epigallocatechin, dicatechin structures, dicatechin structures without a hydroxyl group, galloyl-glucoses and ellagic acid were identified as the main monomeric components in the aqueous extract. 13C-NMR and MALDI-TOF spectra revealed that extracts are mostly composed of procyanidin and prodelphinidin structures although prorobinetidins might be also present. MALDI-TOF spectrometry was used to identify the extract oligomeric components. Extractions with Na2SO3 and/or NaOH produced changes in the predominant structures in the extracts and on the amount of sugar units linked to the flavonoid structures, which decreased in the presence of Na2SO3. Extract obtained using low Na2SO3 and NaOH concentrations (1.5 and 0.75 %, respectively) was selected as more suitable for wood adhesive preparation.


Tannin Ellagic Acid Epigallocatechin Polymerization Degree Epicatechin Gallate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was funded by Ministerio de Ciencia e Innovación, Feder FUNDS and Plan E Fundy (CTQ2009-07539). Authors are also grateful to Ministerio de Ciencia e Innovación for a research grant awarded to J. Santos (BES-2006-13886).

Supplementary material

226_2012_513_MOESM1_ESM.pdf (66 kb)
Supplementary material 1 (PDF 65 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Gonzalo Vázquez
    • 1
  • Antonio Pizzi
    • 2
  • M. Sonia Freire
    • 1
  • Jorge Santos
    • 1
  • Gervasio Antorrena
    • 1
  • Julia González-Álvarez
    • 1
    Email author
  1. 1.Department of Chemical Engineering, School of EngineeringUniversity of Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.ENSTIB-LERMABNancy UniversitéEpinalFrance

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