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Effects of Recombinant Amelogenin on Hydroxyapatite Formation In Vitro

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Abstract.

To determine the role of amelogenin in the mineralization of dental enamel, the effects of the recombinant mouse amelogenin rM179 on in vitro hydroxyapatite formation have been studied. In a steady-state agarose gel assay for hydroxyapatite nucleation, rM179 lacked significant activity at concentrations up to 300 μg/ml. In an autotitration assay for inhibition of de novo hydroxyapatite formation, rM179 had no significant activity at concentrations up to 30 μg/ml. Using selected-area dark-field electron microscopy, it was shown that rM179, at concentrations up to 30 μg/ml, did not significantly affect the length of hydroxyapatite crystals formed in steady-state agarose gels. These findings suggest that amelogenins do not possess the specific crystal-modulating properties characteristic of certain acidic mineralized tissue proteins proteins.

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Authors and Affiliations

  1. School of Dentistry and Department of Biochemistry, University of Western Ontario, London, N6A 5C1, Ontario, Canada, , , , , , CA

    G. K. Hunter & H. A. Curtis

  2. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, M5G 1X5, Canada, , , , , , CA

    M. D. Grynpas

  3. University of Texas School of Dentistry, 7703 Floyd Curl Drive, San Antonio, Texas 78284-7888, USA, , , , , , US

    J. P. Simmer

  4. School of Dentistry, University of Southern California, 2250 Alcazar Street, Los Angeles, California 90033, USA, , , , , , US

    A. G. Fincham

Authors
  1. G. K. Hunter
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  2. H. A. Curtis
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  3. M. D. Grynpas
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  4. J. P. Simmer
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  5. A. G. Fincham
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Received: 2 September 1998 / Accepted: 17 February 1999

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Hunter, G., Curtis, H., Grynpas, M. et al. Effects of Recombinant Amelogenin on Hydroxyapatite Formation In Vitro . Calcif Tissue Int 65, 226–231 (1999). https://doi.org/10.1007/s002239900688

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  • DOI: https://doi.org/10.1007/s002239900688

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