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Femoral Skeletal Perfusion is Reduced in Male Mice with Type 1 Diabetes

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Abstract

The bone vasculature and blood flow are critical for bone modeling, remodeling, and regeneration. Vascular complications are one of the major health concerns of people with type 1 diabetes (T1D). Moreover, people with T1D have lower bone mineral density and increased bone fragility. The goal of this study was to understand whether bone perfusion was altered in a mouse model of T1D and how this related to changes in bone mass. T1D was induced via the administration of streptozotocin in 12-week-old C57BL/6NHsd male mice. The assessment of bone perfusion utilized the injection of fluorescent microspheres with assessment of levels in the bone. Femoral blood flow and VEGF-A expression in the cortical bone shafts were lower in the T1D mice, compared to healthy controls, in this pattern followed that of changes in bone mass. These data demonstrate a possible association between reduced skeletal perfusion and reduced bone mass in the setting of T1D.

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Acknowledgements

GTT was performed by the Center for Diabetes and Metabolic Diseases Core Facility. This research was supported by the National Institutes of Health NIH-NIAMS (5T32AR065971-06 to SUO).

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Author notes

  1. Serra Ucer Ozgurel

    Present address: Department of Nutritional Sciences, University of Texas at Austin, Austin, TX, USA

Authors and Affiliations

  1. Department of Anatomy, Cell Biology, and Physiology, School of Medicine, Indiana University, Indianapolis, IN, USA

    Serra Ucer Ozgurel, Elizabeth A. Swallow, Corinne E. Metzger & Matthew R. Allen

  2. Richard L. Roudebush Veterans Affairs Medical Center, Indianapolis, IN, USA

    Matthew R. Allen

  3. Department of Medicine - Division of Nephrology, Indiana University School of Medicine, Indianapolis, IN, USA

    Matthew R. Allen

  4. Department of Biomedical Engineering, Indiana University Purdue University of Indianapolis, Indianapolis, IN, USA

    Matthew R. Allen

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  1. Serra Ucer Ozgurel
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  4. Matthew R. Allen
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Contributions

SUO and MRA designed the primary study. SUO, EAS, and CEM performed all animal procedures; SUO analyzed the data. SUO drafted the manuscript. All authors reviewed and approved the final version of the manuscript.

Corresponding author

Correspondence to Serra Ucer Ozgurel.

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Conflict of interest

Serra Ucer Ozgurel, Elizabeth A. Swallow, Corinne E. Metzger, Matthew R. Allen have nothing to disclose. Dr. Allen is in the Editorial Board of Calcified Tissue International and Musculoskeletal Research Journal.

Human and Animal Rights and Informed Consent

All procedures involving animals were in compliance with the Institutional Animal Care and Use Committee at Indiana University School of Medicine (Protocol number: 20038).

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Ucer Ozgurel, S., Swallow, E.A., Metzger, C.E. et al. Femoral Skeletal Perfusion is Reduced in Male Mice with Type 1 Diabetes. Calcif Tissue Int 111, 323–330 (2022). https://doi.org/10.1007/s00223-022-00992-y

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  • DOI: https://doi.org/10.1007/s00223-022-00992-y

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