Association of Plasma Irisin with Bone Mineral Density in a Large Chinese Population Using an Extreme Sampling Design
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Irisin, a myokine produced by skeletal muscle in response to physical exercise, promotes trans-differentiation of white adipose tissue into brown adipose tissue. Recent evidences suggested that irisin also plays an important role in the control of bone metabolism. This study aimed to ascertain the relationship between plasma irisin and bone mineral density (BMD) in Chinese population by adoption of an extreme sampling method. Based on a large and screened Chinese elderly population (N = 6308), two subgroups with extremely high and low hip BMD were selected for discovery (N = 80, high vs. low BMD = 44:36) and validation (N = 60, high vs. low BMD = 30:30), respectively. Plasma irisin, P1NP, and β-CTx were measured using commercially available ELISA kits. Other metabolic parameters (e.g., blood glucose, total cholesterol and triglycerides) were collected. Student’s t test and Spearman correlation analyses were conducted in SPSS. Significant difference was discovered for plasma irisin between females and age-matched males (N = 80, male vs. female = 42:38, P = 0.002). The plasma irisin levels were significantly higher in high BMD subjects than in low BMD subjects, which was observed in both discovery (P = 0.012) and validation samples (P = 0.022). However, such observation was limited to males only. Further correlation analyses in males showed that plasma irisin was correlated with BMD (r = 0.362, P = 0.025) and triglyceride (r = − 0.354, P = 0.032). Plasma irisin levels were associated with hip BMD in Chinese elderly men. This study represented the first effort of investigating the relationship of plasma irisin and BMD in elderly population. The positive correlation between plasma irisin and BMD hints intrinsic communication between muscle and bone.
KeywordsIrisin BMD Osteoporosis
The study was supported by Natural Science Foundation of China (81502868, 81373010, 81541068, 81473046, 31271336, 31401079, and 81401343), the Natural Science Foundation of Jiangsu Province (BK20130300, BK20150346), the Natural Science Research Project of Jiangsu Provincial Higher Education (16KJA330001), the Startup Fund from Soochow University (Q413900112, Q413900712), and a Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions.
LFW, DCZ, DFY, and SFL conceived and coordinated the study and wrote the paper. LFW, YHL, and BHW performed and analyzed the experiments. DCZ, CHT, BG, JS, PH, WYW, SQL, JZ, XZ, KZ, WJ, HQG, HBG, XL, LZ, and YHZ provided technical assistance and contributed to the preparation of sample. All authors reviewed the results and approved the final version of the manuscript.
Compliance with Ethical Standards
Conflict of interest
Long-Fei Wu, Dong-Cheng Zhu, Chang-Hua Tang, Bing Ge, Ju Shi, Bing-Hua Wang, Yi-Hua Lu, Pei He, Wen-Yu Wang, Si-Qi Lu, Jiao Zhong, Xu Zhou, Kan Zhu, Wen Ji, Hong-Qin Gao, Hong-Bo Gu, Xing-Bo Mo, Xin Lu, Lei Zhang, Yong-Hong Zhang, Fei-Yan Deng, and Shu-Feng Lei declare no conflict of interest.
Human and Animal Rights and Informed Consent
The study was approved by Institutional Research Ethic Board at the Soochow University. All the participants provided written informed-consent documents before entering the study.
- 1.Laurent MR, Dubois V, Claessens F, Verschueren SMP, Vanderschueren D, Gielen E, Jardi F (2016) Muscle-bone interactions: from experimental models to the clinic? A critical update. Mol Cell Endocrinol 432(C):24–46Google Scholar
- 22.Soininen S, Sidoroff V, Lindi V, Mahonen A, Kroger L, Kroger H, Jaaskelainen J, Atalay M, Laaksonen DE, Laitinen T et al (2018) Body fat mass, lean body mass and associated biomarkers as determinants of bone mineral density in children 6–8 years of age—the Physical Activity and Nutrition in Children (PANIC) study. Bone 108:106–114CrossRefPubMedGoogle Scholar
- 24.Woodhouse A, Black DM (2000) BMD at various sites for the prediction of hip fracture: a meta-analysis. J Bone Miner Res 15:S145–S145Google Scholar
- 25.McCloskey E, Johansson H, Harvey NC, Shepstone L, Lenaghan E, Fordham R, Harvey I, Howe A, Cooper C, Clarke S et al (2018) Management of patients with high baseline hip fracture risk by FRAX reduces hip fractures—a post hoc analysis of the SCOOP study. J Bone Miner Res. https://doi.org/10.1002/jbmr.3411 CrossRefPubMedGoogle Scholar
- 36.Anastasilakis AD, Polyzos SA, Saridakis ZG, Kynigopoulos G, Skouvaklidou EC, Molyvas D, Vasiloglou MF, Apostolou A, Karagiozoglou-Lampoudi T, Siopi A et al (2014) Circulating irisin in healthy, young individuals: day-night rhythm, effects of food intake and exercise, and associations with gender, physical activity, diet, and body composition. J Clin Endocrinol Metab 99(9):3247–3255CrossRefPubMedGoogle Scholar
- 37.Singhal V, Lawson EA, Ackerman KE, Fazeli PK, Clarke H, Lee H, Eddy K, Marengi DA, Derrico NP, Bouxsein ML et al (2014) Irisin levels are lower in young amenorrheic athletes compared with eumenorrheic athletes and non-athletes and are associated with bone density and strength estimates. PLoS ONE 9(6):e100218CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Anastasilakis AD, Polyzos SA, Makras P, Gkiomisi A, Bisbinas I, Katsarou A, Filippaios A, Mantzoros CS (2014) Circulating irisin is associated with osteoporotic fractures in postmenopausal women with low bone mass but is not affected by either teriparatide or denosumab treatment for 3 months. Osteoporosis Int 25(5):1633–1642CrossRefGoogle Scholar