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Irradiation-Induced Compositional Effects on Human Bone After Extracorporeal Therapy for Bone Sarcoma

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Abstract

The present study investigates Raman scattering of human bone irradiated with 50 Gy single dose during therapeutic treatment of Ewing and Osteosarcoma. Bone quality was evaluated via mineral-to-matrix ratio, degree of crystallinity, change in amount of calcium, and carbonate substitution. Alteration in collagen and its cross-links was quantified through second-derivative deconvolution of Amide I peak. A dose of 50 Gy radiation leads to almost 50% loss of mineral content, while maintaining mineral crystallinity, and small changes in carbonate substitution. Deconvolution of Amide I suggested modifications in collagen structure via increase in amount of enzymatic trivalent cross-linking (p < 0.05). Overall irradiation led to detrimental effect on bone quality via changes in its composition, consequently reducing its elastic modulus with increased plasticity. The study thus quantifies effect of single-dose 50 Gy radiation on human bone, which in turn is necessary for designing improved radiation dosage during ECRT and for better understanding post-operative care.

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Funding

This work was supported through graduate student grant of Indian Institute of Technology Delhi and All India Institutes of Medical Sciences New Delhi.

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Authors and Affiliations

  1. Applied Mechanics, Indian Institute of Technology, Delhi, India

    S. Chauhan & A. Prasad

  2. Mechanical Engineering, GBPUAT, Pant Nagar, India

    S. Chauhan

  3. Orthopedics, All India Institute of Medical Sciences, New Delhi, India

    S. A. Khan

  4. Mechanical Engineering, South Dakota State University, Brookings, USA

    A. Prasad

Authors
  1. S. Chauhan
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Correspondence to S. Chauhan.

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S. Chauhan, S. A. Khan, A. Prasad declares that they have no conflict of interest.

Research Involving Human Participants and Informed Consent

All procedures performed in studies involving human participants were in accordance with the ethical standards of the AIIMS review committee dated June 2014. Informed consent was obtained from all individual participants included in the study.

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Chauhan, S., Khan, S.A. & Prasad, A. Irradiation-Induced Compositional Effects on Human Bone After Extracorporeal Therapy for Bone Sarcoma. Calcif Tissue Int 103, 175–188 (2018). https://doi.org/10.1007/s00223-018-0408-2

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