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OPG is Required for the Postnatal Maintenance of Condylar Cartilage

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Abstract

Osteoprotegerin (OPG) is one of the protective factors of bony tissue. However, the function of OPG in cartilage tissues remains elusive. The aim of this study is to explore the function of OPG in the postnatal maintenance and the occurring of osteoarthritis (OA) of temporomandibular joint (TMJ) in the rodent models. We found that OPG expressed in the hypertrophic layer of the condylar cartilage and upregulated in the hyperocclusion-induced-TMJ-trauma rat. In the absence of OPG, the cartilage degradation occurred prior to that in WT mice, and the 3-month-old OPG-Knockout (OPG-KO) condyle showed decreased chondrocyte proliferation and increased chondrocyte apoptosis, whereas the number of chondroclasts was comparable to WT condyle. The isolated chondrocytes from the OPG-KO mice also showed impaired survival and promoted chondrogenic differentiation. Furthermore, the hyperocclusion model deteriorated TMJ degradation in the OPG-KO mice. OPG plays a protective role in the condylar chondrocytes’ survival, and it is required for the postnatal maintenance of TMJ.

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Acknowledgements

We wish to thank Haiming Du and Yunfei Wang for their assistance in animal handling. This study was funded by the National Natural Science Foundation of China.

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  1. Danying Chen and Yi Liu Co-First Authors

Authors and Affiliations

  1. Zhujiang New Town Dental Clinic, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Rd, 510055, Guangzhou, Guangdong, China

    Danying Chen

  2. Department of Endodontics, Xuzhou Stomatological Hospital, 130 Huaihaixi Rd, 221002, Xuzhou, Jiangsu, China

    Yi Liu & Zongxiang Liu

  3. School and Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, 399 Yanchang Rd, 200072, Shanghai, China

    Danying Chen & Yi Liu

  4. Maxillofacial Surgery, Xuzhou Stomatological Hospital, 130 Huaihaixi Rd, 221002, Xuzhou, Jiangsu, China

    Penglai Wang

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DC conceived and designed the study, collected, assembled and interpreted the data, wrote the manuscript. YL collected the data, provided technical supports. ZL and PW provided technical supports. All authors read and approved the final manuscript and agree to be accountable for all aspects of the work.

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Correspondence to Danying Chen.

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Danying Chen, Yi Liu, Zongxiang Liu, and Penglai Wang declare that they have no competing interests.

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Not applicable, as there were no human subjects. All animal protocols were approved by the Animal Use and Care Committee of Tongji University.

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Chen, D., Liu, Y., Liu, Z. et al. OPG is Required for the Postnatal Maintenance of Condylar Cartilage. Calcif Tissue Int 104, 461–474 (2019). https://doi.org/10.1007/s00223-018-00510-z

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