Abstract
Previous work has demonstrated that a single subcutaneous dose of salmon calcitonin leads to a transient decline in circulating levels of FGF23 in patients with X-linked hypophosphatemia (XLH). Since the calcitonin receptor is expressed on osteocytes, this raises the possibility that interdicting signals through that receptor could modulate circulating levels of FGF23 in XLH. In the present study, 21 subjects with XLH were randomly assigned to receive either placebo nasal spray or 400 IU of nasal salmon calcitonin daily for three months. On the first and last day of the study, serial measurements of FGF23, 1,25-dihydroxyvitamin D, and TmP/GFR were made over 27 h. At the beginning of Visit 2 (the first day of month 2) and the beginning of Visit 3 (the first day of month 3), single, first-morning, fasting measurements of these same parameters were made before the next administered dose of study drug. Following the initial or final dose of study drug, there were no differences in area under the curve, based on treatment assignment, for the three principal outcome variables. Similarly, there were no differences in the fasting measures taken at the beginning of Visit 2 or Visit 3 compared to the fasting values on either day 2 of Visit 1 or the fasting values on day 2 of Visit 4. There were also no significant changes over time in serum phosphorus, serum calcium, circulating levels of PTH, CTx, or P1NP. The reasons why nasal salmon calcitonin did not recapitulate the findings with subcutaneously administered drug may relate to the kinetics of drug delivery, the bioavailability of drug or peak drug dose achieved. It remains possible, however, that other means of altering calcitonin receptor signaling may still provide an opportunity for regulating FGF23 production.
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Acknowledgements
The intact FGF23 assay kits were a generous gift from Kyowa Hakko Kirin Co. Ltd. This work was supported by the Yale Bone Center. It was also supported by CTSA Grant Number UL1 TR000142 from the National Center for Advancing Translational Science (NCATS), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NIH.
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Study design: KI and AA. Data collection: KI, TC, AA, CS, RS, EO. Data analysis: YD, CC, RS, KI, AA, CS. Data interpretation: KI, RS, YD and CC. Drafting manuscript: RS, KI. Approving final version of manuscript: RS, KI, TC, AA, CS, YD, and CC. KI assumes responsibility for the integrity of the data analysis.
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Rebecca Sullivan, Alice Abraham, Christine Simpson, Elizabeth Olear, Thomas Carpenter, Yanhong Deng, Chuqing Chen, Karl L. Insogna declare that they have no conflicts of interest relevant to this study.
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This study was approved by the Yale Human Research Protection Program. All study participants provided written informed consent before study entry.
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Sullivan, R., Abraham, A., Simpson, C. et al. Three-Month Randomized Clinical Trial of Nasal Calcitonin in Adults with X-linked Hypophosphatemia. Calcif Tissue Int 102, 666–670 (2018). https://doi.org/10.1007/s00223-017-0382-0
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DOI: https://doi.org/10.1007/s00223-017-0382-0