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A Natural, Calcium-Rich Marine Multi-mineral Complex Preserves Bone Structure, Composition and Strength in an Ovariectomised Rat Model of Osteoporosis

Calcified Tissue International volume 101, pages 445–455 (2017)Cite this article

Abstract

Calcium supplements are used as an aid in the prevention of osteopenia and osteoporosis and also for the treatment of patients when used along with medication. Many of these supplements are calcium carbonate based. This study compared a calcium-rich, marine multi-mineral complex (Aquamin) to calcium carbonate in an ovariectomised rat model of osteoporosis in order to assess Aquamin’s efficacy in preventing the onset of bone loss. Animals were randomly assigned to either non-ovariectomy control (Control), ovariectomy (OVX) plus calcium carbonate, ovariectomy plus Aquamin or ovariectomy plus Aquamin delay where Aquamin treatment started 8 weeks post OVX. At the end of the 20-week study, the trabecular architecture was measured using micro computed tomography, bone composition was assessed using Fourier transform infrared spectroscopy and the mechanical properties were assessed using nanoindentation and three-point bend testing. The study demonstrates that oral ingestion of Aquamin results in less deterioration of trabecular bone structure, mineral composition and tissue level biomechanical properties in the tibia of rats following ovariectomy than calcium carbonate. This study has shown that in an animal model of osteoporosis, Aquamin is superior to calcium carbonate at slowing down the onset of bone loss.

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References

  1. Johnell O, Kanis JA (2006) An estimate of the worldwide prevalence and disability associated with osteoporotic fractures. Osteoporos Int 17(12):1726–1733

    CAS  Article  PubMed  Google Scholar 

  2. Kanis JA, Johnell O (2005) Requirements for DXA for the management of osteoporosis in Europe. Osteoporos Int 16(3):229–238

    CAS  Article  PubMed  Google Scholar 

  3. Reeve J, Meunier PJ, Parsons JA et al (1980) Anabolic effect of human parathyroid hormone fragment on trabecular bone in involutional osteoporosis: a multicentre trial. Br Med J 280(6228):1340–1344

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. Chapuy MC, Arlot ME, Duboeuf F, Brun J, Crouzet B, Arnaud S, Delmas PD, Meunier PJ (1992) Vitamin D3 and calcium to prevent hip fractures in the elderly women. N Engl J Med 327(23):1637–1642

    CAS  Article  PubMed  Google Scholar 

  5. Tang BM, Eslick GD, Nowson C, Smith C, Bensoussan A (2007) Use of calcium or calcium in combination with vitamin D supplementation to prevent fractures and bone loss in people aged 50 years and older: a meta-analysis. Lancet 370(9588):657–666

    CAS  Article  PubMed  Google Scholar 

  6. Institute of Medicine (2011) Dietary reference intakes for calcium and vitamin D. In: Ross AC, Taylor CL, Yaktine AL, Del Valle HB (eds). National Academies Press, Washington DC

  7. Bolland MJ, Leung W, Tai V, Bastin S, Gamble GD, Grey A, Reid IR (2015) Calcium intake and risk of fracture: systematic review. Br Med J 351:h4580

    Article  Google Scholar 

  8. Asmus HG, Braun J, Krause R, Brunkhorst R, Holzer H, Schulz W, Neumayer HH, Raggi P, Bommer J (2005) Two year comparison of sevelamer and calcium carbonate effects on cardiovascular calcification and bone density. Nephrol Dial Transplant 20(8):1653–1661

    CAS  Article  PubMed  Google Scholar 

  9. Bolland MJ, Barber PA, Doughty RN, Mason B, Horne A, Ames R, Gamble GD, Grey A, Reid IR (2008) Vascular events in healthy older women receiving calcium supplementation: randomised controlled trial. Br Med J 336(7638):262–266

    CAS  Article  Google Scholar 

  10. Palacios C (2006) The role of nutrients in bone health, from A to Z. Crit Rev Food Sci Nutr 46(8):621–628

    CAS  Article  PubMed  Google Scholar 

  11. Brennan O, Stenson B, Widaa A, O’Gorman DM, O’Brien FJ (2015) Incorporation of the natural marine multi-mineral dietary supplement Aquamin enhances osteogenesis and improves the mechanical properties of a collagen-based bone graft substitute. J Mech Behav Biomed Mater 47:114–123

    CAS  Article  PubMed  Google Scholar 

  12. O’Gorman DM, Tierney CM, Brennan O, O’Brien FJ (2012) The marine-derived, multi-mineral formula, Aquamin, enhances mineralisation of osteoblast cells in vitro. Phytother Res 26(3):375–380

    PubMed  Google Scholar 

  13. Widaa A, Brennan O, O’Gorman DM, O’Brien FJ (2014) The osteogenic potential of the marine-derived multi-mineral formula aquamin is enhanced by the presence of vitamin D. Phytother Res 28(5):678–684

    CAS  Article  PubMed  Google Scholar 

  14. Aslam MN, Kreider JM, Paruchuri T, Bhagavathula N, DaSilva M, Zernicke RF, Goldstein SA, Varani J (2010) A mineral-rich extract from the red marine algae Lithothamnion calcareum preserves bone structure and function in female mice on a Western-style diet. Calcif Tiss Int 86(4):313–324

    CAS  Article  Google Scholar 

  15. Nielsen BD, Cate RE, O’Connor-Robison CI (2010) A marine mineral supplement alters markers of bone metabolism in yearling Arabian horses. J Equine Vet Sci 30(8):419–424

    Article  Google Scholar 

  16. Brennan O, Kennedy OD, Lee TC, Rackard SM, O’Brien FJ (2009) Biomechanical properties across trabeculae from the proximal femur of normal and ovariectomised sheep. J Biomech 42(4):498–503

    CAS  Article  PubMed  Google Scholar 

  17. Boskey A, Pleshko Camacho N (2007) FT-IR imaging of native and tissue-engineered bone and cartilage. Biomaterials 28(15):2465–2478

    CAS  Article  PubMed  Google Scholar 

  18. Meiron OE, Bar-David E, Aflalo ED, Shechter A, Stepensky D, Berman A, Sagi A (2011) Solubility and bioavailability of stabilized amorphous calcium carbonate. J Bone Miner Res 26(2):364–372

    CAS  Article  PubMed  Google Scholar 

  19. Castiglioni S, Cazzaniga A, Albisetti W, Maier JA (2013) Magnesium and osteoporosis: current state of knowledge and future research directions. Nutrients 5(8):3022–3033

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. Rude RK, Singer FR, Gruber HE (2009) Skeletal and hormonal effects of magnesium deficiency. J Am Coll Nutr 28(2):131–141

    CAS  Article  PubMed  Google Scholar 

  21. Jehle S, Zanetti A, Muser J, Hulter HN, Krapf R (2006) Partial neutralization of the acidogenic Western diet with potassium citrate increases bone mass in postmenopausal women with osteopenia. J Am Soc Nephrol 17(11):3213–3222

    CAS  Article  PubMed  Google Scholar 

  22. Opsahl W, Zeronian H, Ellison M, Lewis D, Rucker RB, Riggins RS (1982) Role of copper in collagen cross-linking and its influence on selected mechanical properties of chick bone and tendon. J Nutr 112(4):708–716

    CAS  PubMed  Google Scholar 

  23. Marie PJ, Ammann P, Boivin G, Rey C (2001) Mechanisms of action and therapeutic potential of strontium in bone. Calcif Tissue Int 69(3):121–129

    CAS  Article  PubMed  Google Scholar 

  24. Fairweather-Tait S, Hurrell RF (1996) Bioavailability of minerals and trace elements. Nutr Res Rev 9(1):295–324

    CAS  Article  PubMed  Google Scholar 

  25. Boskey A, Mendelsohn R (2005) Infrared analysis of bone in health and disease. J Biomed Opt 10(3):031102

    Article  PubMed  Google Scholar 

  26. Brennan O, Kennedy OD, Lee TC, Rackard SM, O’Brien FJ, McNamara LM (2011) The effects of estrogen deficiency and bisphosphonate treatment on tissue mineralisation and stiffness in an ovine model of osteoporosis. J Biomech 44(3):386–390

    CAS  Article  PubMed  Google Scholar 

  27. Brouwers JE, van Rietbergen B, Huiskes R, Ito K (2009) Effects of PTH treatment on tibial bone of ovariectomized rats assessed by in vivo micro-CT. Osteoporos Int 20(11):1823–1835

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  28. Sheng ZF, Dai RC, Wu XP, Fang LN, Fan HJ, Liao EY (2007) Regionally specific compensation for bone loss in the tibial trabeculae of estrogen-deficient rats. Acta Radiol 48(5):531–539

    CAS  Article  PubMed  Google Scholar 

  29. Seeman E (2003) Reduced bone formation and increased bone resorption: rational targets for the treatment of osteoporosis. Osteoporos Int 14(Suppl 3):S2–S8

    Article  PubMed  Google Scholar 

  30. Cummings SR, Cosman F, Jamal SA (2002) Osteoporosis: an evidence-based guide to prevention and management. American College of Physicians, Philadelphia

    Google Scholar 

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Acknowledgements

We thank Marigot Ltd (Cork, Ireland) as the source of the mineral-rich algae extract and for funding this study. We thank Mr. Peter O’Reilly, Senior Experimental Officer in Mechanical and Manufacturing Engineering, Trinity College Dublin for assistance with micro computed tomography, nanoindentation and three-point bend testing.

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Affiliations

  1. Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin 2, Ireland

    Orlaith Brennan

  2. Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, Ireland

    Orlaith Brennan, Joseph Sweeney, Brian O’Meara, Amro Widaa & Fergal J. O’Brien

  3. Trinity Centre for Bioengineering, Trinity College Dublin, Dublin 2, Ireland

    Orlaith Brennan, Amro Widaa & Fergal J. O’Brien

  4. Advanced Materials and BioEngineering Research Centre (AMBER), Trinity College Dublin and Royal College of Surgeons in Ireland, Dublin 2, Ireland

    Orlaith Brennan & Fergal J. O’Brien

  5. EA 6295 Nanomédicaments et Nanosondes, UFR Sciences Pharmaceutiques, Université François-Rabelais de Tours, 31 avenue Monge, 37200, Tours, France

    Franck Bonnier

  6. FOCAS Research Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland

    Hugh J. Byrne

  7. Marigot Ltd, Strand Farm, Currabinny, Carrigaline, Co. Cork, Ireland

    Denise M. O’Gorman

Authors
  1. Orlaith Brennan
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  2. Joseph Sweeney
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  3. Brian O’Meara
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  4. Amro Widaa
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  5. Franck Bonnier
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  6. Hugh J. Byrne
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  7. Denise M. O’Gorman
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  8. Fergal J. O’Brien
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Contributions

Orlaith Brennan, Denise O’Gorman and Fergal O’Brien were responsible for the study design. Orlaith Brennan prepared the first draft of the paper. Orlaith Brennan, Joseph Sweeney, Brian O’Meara, Amro Widaa, Franck Bonnier and Hugh Byrne all contributed to the experimental work. Orlaith Brennan and Joseph Sweeney were responsible for statistical analysis of the data. All authors revised the paper critically for intellectual content and approved the final version. All authors agree to be accountable for the work and to ensure that any questions relating to the accuracy and integrity of the paper are investigated and properly resolved.

Corresponding authors

Correspondence to Orlaith Brennan or Denise M. O’Gorman.

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Conflicts of interest

Dr. O’Gorman is an employee of Marigot Ltd. Prof O’Brien and Dr. Brennan have received funding from Marigot Ltd. to conduct research. Prof Byrne, Dr. Bonnier, Dr. Widaa, Mr. Sweeney and Mr. O’Meara have no disclosures.

Human and Animal Rights and Informed Consent

All applicable international, national and institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Brennan, O., Sweeney, J., O’Meara, B. et al. A Natural, Calcium-Rich Marine Multi-mineral Complex Preserves Bone Structure, Composition and Strength in an Ovariectomised Rat Model of Osteoporosis. Calcif Tissue Int 101, 445–455 (2017). https://doi.org/10.1007/s00223-017-0299-7

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  • DOI: https://doi.org/10.1007/s00223-017-0299-7

Keywords

  • Osteoporosis
  • Bone
  • Bone strength
  • Bone composition
  • Calcium
  • Animal model