A Root-Based Combination Supplement Containing Pueraria lobata and Rehmannia glutinosa and Exercise Preserve Bone Mass in Ovariectomized Rats Fed a High-Fat Diet

Abstract

The aim of this study was to evaluate the effects of a supplement containing Pueraria lobata/Rehmannia glutinosa (PR) root extracts on bone turnover in ovariectomized (OVX) rats (a model for postmenopausal osteoporosis). Female Sprague–Dawley rats (8 weeks old) were randomized into eight groups: sham-operated rats with low-fat control diet + vehicle, OVX rats with low-fat control diet + vehicle, OVX rats with high-fat diet (HFD) + vehicle, OVX rats with HFD + vehicle + exercise, OVX rats with HFD + PR (400 mg/kg body weight/day p.o.), OVX rats with HFD + PR + exercise, OVX rats with HFD + 17β-estradiol (0.5 mg/kg body weight/day p.o.), OVX rats with HFD + 17β-estradiol + exercise. Bone microarchitecture, bone turnover markers (e.g., plasma alkaline phosphatase and osteocalcin), expressions of osteogenic and resorptive gene markers in the bone were measured. Eight weeks of PR and/or aerobic exercise improved cortical microarchitecture of the femur and decreased markers of bone turnover and expression of skeletal osteoclastogenic genes in the femur. PR supplementation combined with exercise preserved bone loss induced by estrogen deficiency and should be investigated further as an alternative to hormone replacement therapy for preventing osteoporosis in postmenopausal women.

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Acknowledgments

This work has been supported by the R&D program of MOTIE/KIAT (Establishment of Infra Structure for Anti-aging Industry Support, #N0000697) and the Ministry of Science, ICT and Future Planning through the National Research Foundation (Bio-synergy Research Project NRF2013M3A9C4078153).

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Correspondence to Oran Kwon.

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Hyang M. Ok, Meron R. Gebreamanuel, Sanga Oh, Hyejin Jeon, Won J. Lee, and Oran Kwon declare no conflict of interest.

Human and Animal Rights and Informed Consent

All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Ewha Womans University, Seoul, Korea. Permit Number: 14-038.

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Ok, H. ., Gebreamanuel, M.R., Oh, S.A. et al. A Root-Based Combination Supplement Containing Pueraria lobata and Rehmannia glutinosa and Exercise Preserve Bone Mass in Ovariectomized Rats Fed a High-Fat Diet. Calcif Tissue Int 97, 624–633 (2015). https://doi.org/10.1007/s00223-015-0057-7

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Keywords

  • Ovariectomy
  • Postmenopausal
  • Osteoporosis
  • Pueraria lobata
  • Rehmannia glutinosa
  • Exercise