Studying Variations in Bone Composition at Nano-Scale Resolution: A Preliminary Report
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Bone has a hierarchical structure extending from the micrometer to the nanometer scale. We report here the first analysis of non-human primate osteonal bone obtained using a spectrometer coupled to an AFM microscope (AFM-IR), with a resolution of 50–100 nm. Average spectra correspond to those observed with conventional FTIR spectroscopy. The following validated FTIR parameters were calculated based on intensities observed in scans covering ~60 µm from the osteon center: mineral content (1030/1660 cm−1), crystallinity (1030/1020 cm−1), collagen maturity (1660/1690 cm−1), and acid phosphate content (1128/1096 cm−1). A repeating pattern was found in most of these calculated IR parameters corresponding to the reported inter- and intra-lamellar spacing in human bone, indicating that AFM-IR measurements will be able to provide novel compositional information on the variation in bone at the nanometer level.
KeywordsAFM-IR Nano-IR Bone composition Osteonal bone Bone nano-structure
Data for this study were collected at Anasys Instruments, Santa Barbara, CA. The study was supported by NIH grant AR041325. The authors are grateful to Dr. Judah Gerstein for editing the manuscript.
Conflict of Interest
Curt Marcott is a scientific advisor for Anasys Instruments; Qichi Hu is an employee of Anasys Instruments; Samuel Gourion-Arsiquaud and Adele Boskey have no conflicts of interest.
Human and Animal Rights and Informed Consent
The animal was from the colony at the Southwest National Primate Research Center/Southwest Foundation for Biomedical Research (SNPRC/SFBR, San Antonio, TX), and all procedures during its life at SNPRC/SFBR were approved by the Institutional Animal Care and Use Committee (IACUC) in accordance with established guidelines. The IACUC of the Hospital for Special Surgery approved the use of these biopsies.
- 4.Donnelly E, Meredith DS, Nguyen JT, Gladnick BP, Rebolledo BJ, Shaffer AD, Lorich DG, Lane JM, Boskey AL (2012) Reduced cortical bone compositional heterogeneity with bisphosphonate treatment in postmenopausal women with intertrochanteric and subtrochanteric fractures. J Bone Miner Res 27:672–678PubMedCrossRefGoogle Scholar
- 5.Gourion-Arsiquaud S, Lukashova L, Power J, Loveridge N, Reeve J, Boskey AL (2013) Fourier transform infrared imaging of femoral neck bone: reduced heterogeneity of mineral-to-matrix and carbonate-to-phosphate and more variable crystallinity in treatment-naive fracture cases compared with fracture-free controls. J Bone Miner Res 28:150–161PubMedCrossRefPubMedCentralGoogle Scholar
- 7.Zoehrer R, Roschger P, Paschalis EP, Hofstaetter JG, Durchschlag E, Fratzl P, Phipps R, Klaushofer K (2006) Effects of 3- and 5-year treatment with risedronate on bone mineralization density distribution in triple biopsies of the iliac crest in postmenopausal women. J Bone Miner Res 21:1106–1112PubMedCrossRefGoogle Scholar
- 9.Misof BM, Roschger P, Gabriel D, Paschalis EP, Eriksen EF, Recker RR, Gasser JA, Klaushofer K (2013) Annual intravenous zoledronic acid for 3 years increased cancellous bone matrix mineralization beyond normal values in the HORIZON biopsy cohort. J Bone Miner Res 28:442–448PubMedCrossRefGoogle Scholar