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Relationship of Bone Mineralization Density Distribution (BMDD) in Cortical and Cancellous Bone Within the Iliac Crest of Healthy Premenopausal Women

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Abstract

Bone mineralization density distribution (BMDD) is an important determinant of bone mechanical properties. The most available skeletal site for access to the BMDD is the iliac crest. Compared to cancellous bone much less information on BMDD is available for cortical bone. Hence, we analyzed complete transiliac crest bone biopsy samples from premenopausal women (n = 73) aged 25–48 years, clinically classified as healthy, by quantitative backscattered electron imaging for cortical (Ct.) and cancellous (Cn.) BMDD. The Ct.BMDD was characterized by the arithmetic mean of the BMDD of the cortical plates. We found correlations between Ct. and Cn. BMDD variables with correlation coefficients r between 0.42 and 0.73 (all p < 0.001). Additionally to this synchronous behavior of cortical and cancellous compartments, we found that the heterogeneity of mineralization densities (Ct.CaWidth), as well as the cortical porosity (Ct.Po) was larger for a lower average degree of mineralization (Ct.CaMean). Moreover, Ct.Po correlated negatively with the percentage of highly mineralized bone areas (Ct.CaHigh) and positively with the percentage of lowly mineralized bone areas (Ct.CaLow). In conclusion, the correlation of cortical with cancellous BMDD in the iliac crest of the study cohort suggests coordinated regulation of bone turnover between both bone compartments. Only in a few cases, there was a difference in the degree of mineralization of >1wt % between both cortices suggesting a possible modeling situation. This normative dataset of healthy premenopausal women will provide a reference standard by which disease- and treatment-specific effects can be assessed at the level of cortical bone BMDD.

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Acknowledgments

The authors thank G. Dinst, D. Gabriel, P. Messmer, and S. Thon for careful sample preparation and qBEI measurements at the Bone Material Laboratory of the Ludwig Boltzmann Institute of Osteology, Vienna, Austria. This work was supported by the AUVA (Austrian Social Insurance for Occupational Risk), the WGKK (Social Health Insurance Vienna), and the NIH Grants AR41386 and DK32333.

Conflict of Interest

Dr. J. P. Bilezikian received institutional Grant support from NPS and Amgen, and is consultant for NPS, Amgen, Merck, Lilly, Radius, and Johnson & Johnson, outside the submitted work. B. M. Misof, D. W. Dempster, Hua Zhou, P. Roschger, N. Fratzl-Zelman, P. Fratzl, S. J. Silverberg, E. Shane, A. Cohen, E. Stein, T. L. Nickolas, R. R. Recker, J. Lappe, and K. Klaushofer declare that they have nothing to disclose.

Human and Animal Rights and Informed Consent

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study.

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Authors and Affiliations

  1. Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, First Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, 1140, Vienna, Austria

    B. M. Misof, P. Roschger, N. Fratzl-Zelman & K. Klaushofer

  2. Regional Bone Center, Helen Hayes Hospital, West Haverstraw, New York, NY, USA

    D. W. Dempster & Hua Zhou

  3. College of Physicians and Surgeons, Columbia University, New York, NY, USA

    D. W. Dempster

  4. Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany

    P. Fratzl

  5. Department of Medicine and Pathology, Columbia University, New York, NY, USA

    S. J. Silverberg, E. Shane, A. Cohen, E. Stein, T. L. Nickolas & J. P. Bilezikian

  6. Creighton University Osteoporosis Research Center, Omaha, NE, USA

    R. R. Recker & J. Lappe

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  1. B. M. Misof
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  2. D. W. Dempster
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  3. Hua Zhou
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Misof, B.M., Dempster, D.W., Zhou, H. et al. Relationship of Bone Mineralization Density Distribution (BMDD) in Cortical and Cancellous Bone Within the Iliac Crest of Healthy Premenopausal Women. Calcif Tissue Int 95, 332–339 (2014). https://doi.org/10.1007/s00223-014-9901-4

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  • DOI: https://doi.org/10.1007/s00223-014-9901-4

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