Abstract
A few clinical studies have reported that elderly male participants with hypertensive disease frequently have higher bone mineral density (BMD) than the normotensive participants at several skeletal sites. The detailed mechanism is still unknown; therefore, a study of bone structure and density using the hypertensive animal models could be informative. We used micro-computed tomography to quantitatively evaluate the tibial and 3rd lumbar vertebral bones in the 20-month-old male spontaneous hypertensive rat (SHR). The BMD, volume fraction, and the microarchitecture changes of the SHR were compared to those of same-age normotensive controls (Wistar-Kyoto rat, WKY). We found that in the very old (20 month) male rats, the trabecular bone fraction and microstructure were higher than those in the same-age normotensive controls. The observation of the association of hypertension with BMD and bone strength in hypertensive rats warrants further investigations of bone mass and strength in elderly males with hypertension.
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Acknowledgments
The authors would like to thanks Dr. Kathleen Brennan (LBNL) and Ms. Stephanie Murphy (UCSF, Radiology) for handling the serum test and harvesting the bone samples. The study was supported in part by the National Institutes of Health of the U. S. Department of Health and Human Services under grants R01 EB007219 (GTG), R01 AG17762 (SM), and R01 EB012965 (YS), and by the Director, Office of Science, Office of Biological and Environmental Research of the U. S. Department of Energy under contract DE-AC02-05CH11231 (GTG).
Human and Animal Rights and Informed Consent
All in vivo animal studies in this study were performed in accordance with the protocols approved by Institutional Animal Care and Use Committee (IACUC) from both University of California, San Francisco (UCSF) and Lawrence Berkeley National Laboratory (LBNL).
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Lee, TC., Burghardt, A.J., Yao, W. et al. Improved Trabecular Bone Structure of 20-Month-Old Male Spontaneously Hypertensive Rats. Calcif Tissue Int 95, 282–291 (2014). https://doi.org/10.1007/s00223-014-9893-0
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DOI: https://doi.org/10.1007/s00223-014-9893-0