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Parathyroid Hormone (1-34) Modulates Odontoblast Proliferation and Apoptosis via PKA and PKC-Dependent Pathways

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Abstract

Parathyroid hormone (PTH) plays a key role in the development and homeostasis of mineralized tissues such as bone and dentine. We have reported that PTH (1-34) administration can increase dentine formation in mice and that this hormone modulates in vitro mineralization of odontoblast-like cells. The purpose of the present study was to investigate whether PTH (1-34) participates in the proliferative and apoptotic signaling of odontoblast-like cells (MDPC23). MDPC23 cells were exposed to 50 ng/ml hPTH (1-34) or vehicle for 1 (P1), 24 (P24), or 48 (P48) hours, and the cell proliferation, apoptosis, and cell number were evaluated. To examine whether changes in the proliferative and apoptotic signaling in response to PTH involve protein kinases A (PKA) and/or C (PKC), MDPC23 cells were exposed to PTH with or without PKC or PKA signaling pathway inhibitors. Overall, the results showed that the PKA pathway acts in response to PTH exposure maintaining levels of cell proliferation, while the PKC pathway is mainly involved for longer exposure to PTH (24 or 48 h), leading to the reduction of cell proliferation and increase of apoptosis. The exposure to PTH reduced the cell number in relation to the control group in a time-dependent manner. In conclusion, PTH modulates odontoblast-like cell proliferative and apoptotic response in a time-dependent manner. Both PKC and PKA pathways participate in PTH-induced modulation in an antagonist mode.

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Acknowledgments

São Paulo State Research Foundation supported this project (2009/06125-4). Dr. Marques is supported by Capes.

Human and Animal Rights and Informed Consent

This article does not contain any studies with human or animal subjects performed by any of the authors.

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Authors and Affiliations

  1. Department of Morphology, Division of Histology and Embryology, Piracicaba Dental School, University of Campinas, Av. Limeira 901, Caixa Postal 052 CEP, Piracicaba, São Paulo, 13414–903, Brazil

    Gustavo Narvaes Guimarães, Ana Paula de Souza, Sergio Roberto Line & Marcelo Rocha Marques

  2. Department of Stomatological Sciences, School of Dentistry, Federal University of Goiás, Goiânia, Goiás, Brazil

    Thaisângela Lopes Rodrigues

Authors
  1. Gustavo Narvaes Guimarães
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  2. Thaisângela Lopes Rodrigues
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  3. Ana Paula de Souza
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  4. Sergio Roberto Line
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  5. Marcelo Rocha Marques
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Correspondence to Marcelo Rocha Marques.

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The authors declare that they have no conflict of interest.

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Guimarães, G.N., Rodrigues, T.L., de Souza, A.P. et al. Parathyroid Hormone (1-34) Modulates Odontoblast Proliferation and Apoptosis via PKA and PKC-Dependent Pathways. Calcif Tissue Int 95, 275–281 (2014). https://doi.org/10.1007/s00223-014-9892-1

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  • DOI: https://doi.org/10.1007/s00223-014-9892-1

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