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Circulating FGF23 Levels in Response to Acute Changes in Plasma Ca2+

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Abstract

The regulation of fibroblast growth factor 23 (FGF23) synthesis and secretion is still incompletely understood. FGF23 is an important regulator of renal phosphate excretion and has regulatory effects on the calciotropic hormones calcitriol and parathyroid hormone (PTH). Calcium (Ca) and phosphate homeostasis are closely interrelated, and it is therefore likely that Ca is involved in FGF23 regulation. It has recently been reported that dietary Ca influenced FGF23 levels, with high Ca increasing FGF23. The mechanism remains to be clarified. It remains unknown whether acute changes in plasma Ca influence FGF23 levels and whether a close relationship, similar that known for Ca and PTH, exists between Ca and FGF23. Thus, the aim of the present study was to examine whether acute hypercalcemia and hypocalcemia regulate FGF23 levels in the rat. Acute hypercalcemia was induced by an intravenous Ca infusion and hypocalcemia by infusion of ethylene glycol tetraacetic acid (EGTA) in normal and acutely parathyroidectomized rats. Intact plasma FGF23 and intact plasma PTH and plasma Ca2+ and phosphate were measured. Acute hypercalcemia and hypocalcemia resulted as expected in adequate PTH secretory responses. Plasma FGF23 levels remained stable at all plasma Ca2+ levels; acute parathyroidectomy did not affect FGF23 secretion. In conclusion, Ca is not a regulator of acute changes in FGF23 secretion.

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Acknowledgments

The authors are grateful for the skillful help of technician Kirsten Bang. The study was partly supported by a grant from the Lundbeck Foundation (R126-A12320).

Human and Animal Rights and Informed Consent

The experimental studies were performed in accordance with the National Institute for Health’s Guide for Care and Use of Laboratory Animals and approved by the Animal Experiment Inspectorate, at the Ministry of Food, Agriculture and Fisheries, in Denmark (Reference: 2012-DY-2934-00023).

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Authors and Affiliations

  1. Nephrological Department P, Rigshospitalet, University of Copenhagen, 2100, Copenhagen, Denmark

    Eva Gravesen, Maria L. Mace, Jacob Hofman-Bang & Klaus Olgaard

  2. Nephrological Department B, Herlev Hospital, University of Copenhagen, 2730, Copenhagen, Denmark

    Maria L. Mace & Ewa Lewin

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  1. Eva Gravesen
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  2. Maria L. Mace
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  3. Jacob Hofman-Bang
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Correspondence to Ewa Lewin.

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Gravesen, E., Mace, M.L., Hofman-Bang, J. et al. Circulating FGF23 Levels in Response to Acute Changes in Plasma Ca2+ . Calcif Tissue Int 95, 46–53 (2014). https://doi.org/10.1007/s00223-014-9861-8

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