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Acidosis Inhibits Mineralization in Human Osteoblasts

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Abstract

Osteoblasts and osteoclasts maintain bone volume. Acidosis affects the function of these cells including mineral metabolism. We examined the effect of acidosis on the expression of transcription factors and mineralization in human osteoblasts in vitro. Human osteoblasts (SaM-1 cells) derived from the ulnar periosteum were cultured with α-MEM containing 50 μg/ml ascorbic acid and 5 mM β-glycerophosphate (calcifying medium). Acidosis was induced by incubating the SaM-1 cells in 10 % CO2 (pH approximately 7.0). Mineralization, which was augmented by the calcifying medium, was completely inhibited by acidosis. Acidosis depressed c-Jun mRNA and increased osteoprotegerin (OPG) production in a time-dependent manner. Depressing c-Jun mRNA expression using siRNA increased OPG production and inhibited mineralization. In addition, depressing OPG mRNA expression with siRNA enhanced mineralization in a dose-dependent manner. Acidosis or the OPG protein strongly inhibited mineralization in osteoblasts from neonatal mice. The present study was the first to demonstrate that acidosis inhibited mineralization, depressed c-Jun mRNA expression, and induced OPG production in human osteoblasts. These results suggest that OPG is involved in mineralization via c-Jun in human osteoblasts.

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Acknowledgments

The authors thank Dr. Y. Koshihara for donating the SaM-1 cells. This study was partly supported by a Grant-in-Aid for Scientific Research (20592193 to A.T.) from the Japan Society for the Promotion of Science and by a Grant-in Aid from the Strategic Research AGU-Platform Formation (2008–2012).

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  1. Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, 464-8650, Japan

    Shoko Takeuchi, Koji Hirukawa & Akifumi Togari

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  1. Shoko Takeuchi
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  2. Koji Hirukawa
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  3. Akifumi Togari
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Correspondence to Akifumi Togari.

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The authors report that they have no conflict of interest.

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Takeuchi, S., Hirukawa, K. & Togari, A. Acidosis Inhibits Mineralization in Human Osteoblasts. Calcif Tissue Int 93, 233–240 (2013). https://doi.org/10.1007/s00223-013-9746-2

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  • DOI: https://doi.org/10.1007/s00223-013-9746-2

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