Abstract
Fracture consolidation is a crucial goal to achieve as early as possible, but pharmacological stimulation has been neglected so far. Teriparatide has been considered for this purpose for its anabolic properties. We set up a murine model of closed tibial fracture on which different doses of teriparatide were tested. Closed fracture treatment avoids any bias introduced by surgical manipulations. Teriparatide’s effect on callus formation was monitored during the first 4 weeks from fracture. Callus evolution was determined by histomorphometric and microhardness assessment. Daily administration of 40 μg/kg of teriparatide accelerated callus mineralization from day 9 onward without significant increase of sizes, and at day 15 the microhardness properties of treated callus were similar to those of bone tissue. Teriparatide considerably improved callus consolidation in the very early phases of bone healing.
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Acknowledgements
We thank Prof. A. Angeli for enthusiastically encouraging this study, Eli Lilly for providing teriparatide, and Dr. B. Peracino for assistance in confocal microscopy.
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Mognetti, B., Marino, S., Barberis, A. et al. Experimental Stimulation of Bone Healing with Teriparatide: Histomorphometric and Microhardness Analysis in a Mouse Model of Closed Fracture. Calcif Tissue Int 89, 163–171 (2011). https://doi.org/10.1007/s00223-011-9503-3
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DOI: https://doi.org/10.1007/s00223-011-9503-3