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Progesterone Modulates the Proliferation and Differentiation of Human Periodontal Ligament Cells

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Abstract

Hormone deficiency has been recognized as a risk factor for periodontal disease in postmenopausal women. However, the anabolic effects of progesterone on human periodontal ligament cells (hPDLCs) are still unclear. Therefore, the objective of this study was to detect the expression of progesterone receptor (PgR) in hPDLCs and investigate the bone-sparing effects of progesterone. We detected PgR expression in hPDLCs by reverse transcriptase–polymerase chain reaction and immunocytochemistry. After progesterone stimulation, the percentage of hPDLCs entering the S + G2M phase of the cell cycle increased significantly, accompanied by an increased cell growth curve. In both basic culture medium and osteogenic medium, progesterone activated alkaline phosphatase–positive cells and alizarin red–positive nodules. Moreover, mineralization-related markers were up-regulated by progesterone in both time-dependent and dose-dependent manners. In contrast, these effects of progesterone were blocked by the PgR antagonist (RU486). Our results demonstrated that the PgR is expressed in hPDLCs at the gene and protein level, and that progesterone can stimulate the proliferation and differentiation of the hPDLCs. These findings suggest that progesterone may play a significant role in osteoblastic function of hPDLCs and may influence the maintenance of alveolar bone mass.

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Acknowledgments

This study was supported by Nature Science Foundation of China (grant 30872913). We thank Dr. Jiaxing Zhou for generously providing the MCF-7 cell line. We also acknowledge the guidance and advice of Dr. Liang Tang and Dr. Dongyang Ma.

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Authors and Affiliations

  1. Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi, 710032, China

    Gongjie Yuan, Chuan Cai, Juan Dai, Yali Liu, Yuanyuan Dai, Li Wen & Yin Ding

  2. Department of Orthodontics, Dalian Stomatological Hospital, Dalian, Liaoning, 116002, China

    Gongjie Yuan

  3. Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi’an, Shaanxi, 710032, China

    Yali Liu

  4. Department of Stomatology, Nangang Branch of Heilongjiang Provincial Hospital, Harbin, Heilongjiang, China

    Rui Zhang

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  1. Gongjie Yuan
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Correspondence to Yin Ding.

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Yuan, G., Cai, C., Dai, J. et al. Progesterone Modulates the Proliferation and Differentiation of Human Periodontal Ligament Cells. Calcif Tissue Int 87, 158–167 (2010). https://doi.org/10.1007/s00223-010-9377-9

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  • DOI: https://doi.org/10.1007/s00223-010-9377-9

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