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Vitamin K2 Promotes Bone Healing in a Rat Femoral Osteotomy Model with or without Glucocorticoid Treatment

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Abstract

The purpose of the present preclinical study was to determine whether vitamin K2 would promote bone healing in a rat femoral osteotomy model with or without glucocorticoid (GC) treatment. Thirty-eight 6 week-old female Sprague–Dawley rats underwent a unilateral osteotomy of the femoral diaphysis followed by intramedullary wire fixation and then were randomized into four groups that received the following treatment schedules: vehicle, vitamin K2, GC + vehicle, and GC + vitamin K2. GC (prednisolone, 2.5 mg/kg) was administered subcutaneously twice a week. Vitamin K2 (menatetrenone, 30 mg/kg) was administered orally five times a week. After 8 weeks of treatment, the wires were removed and a bone histomorphometric analysis was performed on the bone tissue inside the callus. Vitamin K2 administration to GC-untreated rats decreased the osteoclast surface/bone surface (OcS/BS), osteoblast surface (ObS)/BS, eroded surface (ES)/BS, and bone formation rate (BFR)/BS and increased the lamellar area/bone area. Although GC treatment increased the ES/BS and decreased the ObS/BS, BFR/BS, and lamellar area/bone area, vitamin K2 administration to GC-treated rats decreased the OcS/BS and prevented an increase in the ES/BS and a decrease in the lamellar area/bone area. These results suggested that vitamin K2 downregulated bone turnover and stimulated lamellar bone formation in GC-untreated rats and prevented an increase in bone resorption while maintaining bone formation and prevented a decrease in lamellar bone formation in GC-treated rats. Thus, vitamin K2 appears to be effective for promoting bone healing in a rat femoral osteotomy model with or without GC treatment.

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Authors and Affiliations

  1. Institute for Integrated Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Jun Iwamoto, Hideo Matsumoto & Tsuyoshi Tadeda

  2. Hamri Co., Ltd., Tokyo, Japan

    Azusa Seki

  3. Department of Neurology, Mitate Hospital, Fukuoka, Japan

    Yoshihiro Sato

  4. Metabolism Laboratory, Department of Medicine, Winthrop-University Hospital, Mineola, NY, USA

    James K. Yeh

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  1. Jun Iwamoto
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  2. Azusa Seki
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  3. Yoshihiro Sato
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  4. Hideo Matsumoto
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  5. Tsuyoshi Tadeda
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  6. James K. Yeh
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Correspondence to Jun Iwamoto.

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Iwamoto, J., Seki, A., Sato, Y. et al. Vitamin K2 Promotes Bone Healing in a Rat Femoral Osteotomy Model with or without Glucocorticoid Treatment. Calcif Tissue Int 86, 234–241 (2010). https://doi.org/10.1007/s00223-010-9333-8

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  • DOI: https://doi.org/10.1007/s00223-010-9333-8

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