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Tetracyclines Inhibit Rat Osteoclast Formation and Activity In Vitro and Affect Bone Turnover in Young Rats In Vivo

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Abstract

An experiment was designed to investigate whether systemic administration of tetracyclines (TCs) as bone fluorochrome labels could interfere with bone modeling in vivo and inhibit osteoclast formation and activity in vitro. Cell cultures of rat bone marrow macrophages revealed that TC and oxytetracycline inhibited osteoclastogenesis and bone resorption and stimulated apoptosis. Forty rats in five groups were treated with saline, calcein green, alizarin red S, TC, or oxytetracycline. Their tibias were used for histomorphometric analysis, including bone static, dynamic, and resorption parameters in the tibial proximal metaphysis. No significant differences in bone volume per tissue volume, trabecular number, trabecular thickness, trabecular separation, bone formation rate per bone surface, mineralizing surface, or mineral apposition rate were observed. TC or oxytetracycline decreased eroded surface, number of osteoclasts per bone perimeter, and osteoclast surface per bone surface by about 50%. The results demonstrated that TC and oxytetracycline inhibit rat osteoclast formation and activity in vitro, and histomorphometric parameters involved in bone turnover may be affected by the use of oxytetracycline and TC as fluorescent bone labels in vivo.

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Acknowledgment

The authors are grateful to Li Deqiang, Ph.D. (Shanghai Jiao Tong University School of Medicine, Shanghai, China), for providing the Annexin V-PI detection kit used in this study.

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Authors and Affiliations

  1. Department of Orthopedics, Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People’s Republic of China

    Xiaoxiao Zhou, Bingchen An & Zhen’an Zhu

  2. Department of Orthopedics, Taizhou Central Hospital, Taizhou, Zhejiang Province, People’s Republic of China

    Xiaoxiao Zhou

  3. Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, People’s Republic of China

    Peng Zhang

  4. Department of Orthopedics, Kunhua Hospital of Kunming Medical College, Kunming, Yunnan Province, People’s Republic of China

    Chao Zhang

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  1. Xiaoxiao Zhou
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  2. Peng Zhang
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Correspondence to Zhen’an Zhu.

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Zhou, X., Zhang, P., Zhang, C. et al. Tetracyclines Inhibit Rat Osteoclast Formation and Activity In Vitro and Affect Bone Turnover in Young Rats In Vivo. Calcif Tissue Int 86, 163–171 (2010). https://doi.org/10.1007/s00223-009-9328-5

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  • DOI: https://doi.org/10.1007/s00223-009-9328-5

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