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TGF-β/Extracellular Matrix Interactions in Dentin Matrix: A Role in Regulating Sequestration and Protection of Bioactivity

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Abstract

TGF-β isoforms sequestrated in dentin matrix potentially provide a reservoir of bioactive molecules that may influence cell behavior in the dentin–pulp complex following tissue injury. The association of these growth factors with dentin matrix and the influence of such associations on the bioactivity of growth factors are still unclear. We used surface plasmon resonance technology in the BIAcoreTM 3000 system to investigate the binding of TGF-β isoforms 1 and 3 to purified decorin, biglycan, and EDTA soluble dentin matrix components. TGF-β isoforms 1 and 3 were immobilized on sensorchips CM4 through amine coupling. For kinetic studies of protein binding, purified decorin and biglycan, isolated EDTA soluble dentin matrix, and dentin matrix immunodepleted of decorin and/or biglycan were injected over TGF-β isoforms and allowed to interact. Programmed kinetic analysis software provided sensorgrams for each concentration of proteoglycan or dentin matrix extract injected. Purified decorin and biglycan and dentin matrix extract bound to the TGF-β isoforms. However, the association with TGF-β3 was much weaker than that with TGF-β1. After immunoaffinity depletion of the dentin matrix extract, the level of interaction between the dentin matrix extract and TGF-β was significantly reduced. These results suggest isoform-specific interactions between decorin/biglycan and TGF-β isoforms 1 and 3, which may explain why TGF-β3 is not detected in the dentin matrix despite being expressed at higher levels than TGF-β1 in odontoblasts. These proteoglycans appear to play a significant role in TGF-β/extracellular matrix interactions and may be important in the sequestration of these growth factors in the dentin matrix.

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Authors and Affiliations

  1. Tissue Injury and Repair, School of Dentistry, University of Birmingham, Birmingham, B4 6NN, UK

    S. M. Baker, A. J. Smith & P. R. Cooper

  2. Centre for Oral Biology, Institute for Odontology, Karolinska Institutet, P.O. Box 4064, 14104, Huddinge, Stockholm, Sweden

    R. V. Sugars & M. Wendel

  3. Mineralised Tissue Group, Tissue Engineering and Regenerative Dentistry, School of Dentistry, Cardiff University, Heath Park, Cardiff, CF14 4XY, UK

    R. J. Waddington & A. J. Sloan

  4. Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, Cardiff University, Heath Park, Cardiff, CF14 4XY, UK

    R. J. Waddington & A. J. Sloan

Authors
  1. S. M. Baker
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  2. R. V. Sugars
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  3. M. Wendel
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  4. A. J. Smith
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  5. R. J. Waddington
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  6. P. R. Cooper
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  7. A. J. Sloan
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Correspondence to A. J. Sloan.

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Baker, S.M., Sugars, R.V., Wendel, M. et al. TGF-β/Extracellular Matrix Interactions in Dentin Matrix: A Role in Regulating Sequestration and Protection of Bioactivity. Calcif Tissue Int 85, 66–74 (2009). https://doi.org/10.1007/s00223-009-9248-4

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  • DOI: https://doi.org/10.1007/s00223-009-9248-4

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