Skip to main content
SpringerLink
Log in

Particle-Induced Osteolysis in Three-Dimensional Micro-Computed Tomography

Calcified Tissue International Aims and scope Submit manuscript

Abstract

Small-animal models are useful for the in vivo study of particle-induced osteolysis, the most frequent cause of aseptic loosening after total joint replacement. Microstructural changes associated with particle-induced osteolysis have been extensively explored using two-dimensional (2D) techniques. However, relatively little is known regarding the 3D dynamic microstructure of particle-induced osteolysis. Therefore, we tested micro-computed tomography (micro-CT) as a novel tool for 3D analysis of wear debris-mediated osteolysis in a small-animal model of particle-induced osteolysis. The murine calvarial model based on polyethylene particles was utilized in 14 C57BL/J6 mice randomly divided into two groups. Group 1 received sham surgery, and group 2 was treated with polyethylene particles. We performed 3D micro-CT analysis and histological assessment. Various bone morphometric parameters were assessed. Regression was used to examine the relation between the results achieved by the two methods. Micro-CT analysis provides a fully automated means to quantify bone destruction in a mouse model of particle-induced osteolysis. This method revealed that the osteolytic lesions in calvaria in the experimental group were affected irregularly compared to the rather even distribution of osteolysis in the control group. This is an observation which would have been missed if histomorphometric analysis only had been performed, leading to false assessment of the actual situation. These irregularities seen by micro-CT analysis provide new insight into individual bone changes which might otherwise be overlooked by histological analysis and can be used as baseline information on which future studies can be designed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (France)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

References

  1. Silva MJ, Sandell LJ (2002) What’s new in orthopaedic research. J Bone Joint Surg Am 84:1490–1496

    PubMed  Google Scholar 

  2. Willert HG, Bertram H, Buchhorn GH (1990) Osteolysis in alloarthroplasty of the hip. The role of ultra-high molecular weight polyethylene wear particles. Clin Orthop 258:95–107

    PubMed  Google Scholar 

  3. Shanbhag AS, Hasselman CT, Rubash HE (1997) The John Charnley Award. Inhibition of wear debris mediated osteolysis in a canine total hip arthroplasty model. Clin Orthop 344:33–43

    PubMed  Google Scholar 

  4. Merkel KD, Erdmann JM, McHugh KP, Abu-Amer Y, Ross FP, Teitelbaum SL (1999) Tumor necrosis factor α mediates orthopedic implant osteolysis. Am J Pathol 154:203–210

    PubMed  CAS  Google Scholar 

  5. Schwarz EM, Benz EB, Lu AP, Goater JJ, Mollano AV, Rosier RN, Puzas JE, O’Keefe RJ (2000) Quantitative small animal surrogate to evaluate drug efficacy in preventing wear debris-induced osteolysis. J Orthop Res 18:849–855

    Article  PubMed  CAS  Google Scholar 

  6. Von Knoch M, Sprecher C, Barden B, Saxler G, Loer F, Wimmer M (2004) Grösse und Form kommerziell erhältlicher Polyethylenpartikel für in vitro und in vivo Versuche. Z Orthop 142:366–370

    Article  Google Scholar 

  7. Child LM, Goater JJ, O’Keefe RJ, Schwarz EM (2001) Effect of anti-tumor necrosis factor-alpha gene on wear debris-induced osteolysis. J Bone Joint Surg Am 83:1789–1797

    Google Scholar 

  8. Taki N, Tatro JM, Lower R, Goldberg VM, Greenfield EM (2007) Comparison of the roles of IL-1, IL-6, and TNF-α in cell culture and murine models of aseptic loosening. Bone 40:1276–1283

    Article  PubMed  CAS  Google Scholar 

  9. Von Knoch M, Wedemeyer C, Pingsmann A, Von Knoch F, Hilken G, Sprecher C, Henschke F, Barden B, Löer F (2005) A single dose of zoledronic acid markedly decreases particle-induced bone resorption. Biomaterials 26:1803–1808

    Article  CAS  Google Scholar 

  10. Wedemeyer C, Von Knoch F, Pingsmann A, Hilken G, Sprecher C, Saxler G, Henschke F, Löer F, Von Knoch M (2005) Stimulation of bone formation by zoledronic acid in particle-induced osteolysis. Biomaterials 26:3719–3725

    Article  PubMed  CAS  Google Scholar 

  11. Schwarz EM, Lu AP, Goater JJ, Benz EB, Kollias G, Rosier RN, Puzas JE, O’Keefe RJ (2000) Tumor necrosis factor-alpha/nuclear transcription factor-kappaB signaling in periprosthetic osteolysis. J Orthop Res 18:472–480

    Article  PubMed  CAS  Google Scholar 

  12. Wedemeyer C, Neuerburg C, Pfeiffer A, Heckelei A, Von Knoch F, Hilken G, Brankamp J, Henschke F, Von Knoch M, Loer F, Saxler G (2007) Polyethylene particle-induced bone resorption in substance P deficient mice. Calcif Tissue Int 80:268–274

    Article  PubMed  CAS  Google Scholar 

  13. Wedemeyer C, Neuerburg C, Pfeiffer A, Bylski D, Heckelei A, von Knoch F, Hilken G, Schinke T, Gosheger G, von Knoch M, Löer F, Saxler G (2007) Polyethylene particle induced bone resorption in alpha-calcitonin gene related peptide deficient mice. J Bone Miner Res 22:1011–1019

    Article  PubMed  CAS  Google Scholar 

  14. Feldkamp LA, Davis LC, Kress JW (1984) Practical cone-beam algorithm. J Opt Soc Am 1:612–619

    Article  Google Scholar 

  15. Zhao S, Yu H, Wang G (2005) A unified framework for exact cone-beam reconstruction formulas. Med Phys 32:1712–1721

    Article  PubMed  Google Scholar 

  16. Perilli E, Baruffaldi F (2003) Proposal for shared collections of X-ray micro CT datasets of bone specimens. International Conference of Computional Bioengineering, Zaragoza, Spain

  17. Parfitt AM (1988) Bone histomorphometry: proposed system for standardization of nomenclature, symbols, and units. Calcif Tissue Int 42:284–286

    Article  PubMed  CAS  Google Scholar 

  18. Sinha RK, Shanbhag AS, Maloney WJ, Hasselman CT, Rubash HE (1998) Osteolysis: cause and effect. Instr Course Lect 47:307–320

    PubMed  CAS  Google Scholar 

  19. Willert HG, Semlitsch M (1977) Reactions of the articular capsule to wear products of artificial joint prostheses. J Biomed Mater Res 11:157–164

    Article  PubMed  CAS  Google Scholar 

  20. Kobayashi A, Freeman MAR, Bonfield W, Kadoya Y, Yamac T, Al-Saffar N, Scott G, Revell PA (1997) Number of polyethylene particles and osteolysis in total joint replacements. J Bone Joint Surg Br 79:844–848

    Article  PubMed  CAS  Google Scholar 

  21. Schuh A, Thomas P, Holzwarth U, Zeiler G (2004) Bilateral localized osteolysis after cemented total hip replacement. Orthopade 33:727–732

    PubMed  CAS  Google Scholar 

  22. Park JS, Ryu KN, Hong HP, Park YK, Chun YS, Yoo MC (2004) Focal osteolysis in total hip replacement: CT findings. Skeletal Radiol 33:632–640

    Article  PubMed  Google Scholar 

  23. Uchiyama T, Tanizawa T, Muramatsu H, Endo N, Takahashi HE, Hara T (1997) A morphometric comparison of trabecular structure of human ileum between micro computed tomography and conventional histomorphometry. Calcif Tissue Int 61:493–498

    Article  PubMed  CAS  Google Scholar 

  24. Sran MM, Boyd SK, Cooper DM, Khan KM, Zernicke RF, Oxland TR (2007) Regional trabecular morphology assessed by micro-CT is correlated with failure of aged thoracic vertebrae under a posteroanterior load and may determine the site of fracture. Bone 40:751–757

    Article  PubMed  Google Scholar 

  25. Kinney JH, Lane NE, Haupt DL (1995) In vivo, three-dimensional microscopy of trabecular bone. J Bone Miner Res 10:264–270

    Article  PubMed  CAS  Google Scholar 

  26. Ruegsegger P, Koller B, Muller R (1996) A microtomographic system for the nondestructive evaluation of bone architecture. Calcif Tissue Int 58:24–29

    Article  PubMed  CAS  Google Scholar 

  27. Mitton D, Cendre E, Roux JP, Arlot ME, Peix G, Rumelhart C, Babot D, Meunier PJ (1998) Mechanical properties of ewe vertebral cancellous bone compared with histomorphometry and high-resolution computed tomography parameters. Bone 22:651–658

    Article  PubMed  CAS  Google Scholar 

  28. Yang SY, Yu H, Gong W, Wu B, Mayton L, Costello R, Wooley PH (2007) Murine model of prosthesis failure for the long-term study of aseptic loosening. J Orthop Res 25:603–611

    Article  PubMed  Google Scholar 

  29. Manley MT, Capello WN, D’Antonio JA, Edidin AA, Geesink RG (1998) Fixation of acetabular cups without cement in total hip arthroplasty: a comparison of three different implant surfaces at a minimum duration of follow-up of five years. J Bone Joint Surg Am 80:1175–1185

    PubMed  CAS  Google Scholar 

  30. Capello WN, D’Antonio JA, Feinberg JR, Manley MT (1997) Hydroxyapatite-coated total hip femoral components in patients less than fifty years old: clinical and radiographic results after five to eight years of follow up. J Bone Joint Surg Am 79:1023–1029

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The study was supported by Biomaterialien NRW/Ministerium für Forschung und Wissenschaft. The authors thank Kaye Schreyer for editorial assistance with the manuscript and Priv. Doz. Dr. med. Frank Henschke for preparing the histology.

Author information

Authors and Affiliations

  1. Department of Orthopedics, University of Duisburg-Essen, Hufelandstr. 55, 45122, Essen, Germany

    Christian Wedemeyer, Carl Neuerburg, Stefan Landgraeber, Marius von Knoch, Franz Löer & Guido Saxler

  2. Department of Orthopedics, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

    Jie Xu

  3. Department of Cardiology, University of Duisburg-Essen, Hufelandstr. 55, 45122, Essen, Germany

    Nasser M. Malyar

  4. Schulthess Clinic, Lengghalde 2, 8008, Zürich, Switzerland

    Fabian von Knoch

  5. Department of Orthopedics, University of Münster, Albert-Schweitzer-Str. 33, 48149, Münster, Germany

    Georg Gosheger

Authors
  1. Christian Wedemeyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jie Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carl Neuerburg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stefan Landgraeber
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nasser M. Malyar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fabian von Knoch
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Georg Gosheger
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Marius von Knoch
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Franz Löer
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Guido Saxler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christian Wedemeyer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wedemeyer, C., Xu, J., Neuerburg, C. et al. Particle-Induced Osteolysis in Three-Dimensional Micro-Computed Tomography. Calcif Tissue Int 81, 394–402 (2007). https://doi.org/10.1007/s00223-007-9077-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00223-007-9077-2

Keywords

search

Navigation