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Carbonate Assignment and Calibration in the Raman Spectrum of Apatite

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Abstract

A series of apatites with varying carbonate levels was prepared in order to assign the carbonate bands and calibrate for Raman analysis of natural materials. Overlap of carbonate bands with phosphate peaks was resolved by curve fitting. A peak at 1,071 cm−1 was assigned to a combination of the carbonate ν1 mode at 1,070 cm−1 with a phosphate ν3 mode at 1,076 cm−1. In addition, the carbonate ν4 mode was identified in apatite samples with >4% carbonate. The carbonate ν4 bands at 715 and 689 cm−1 identify the samples as B-type carbonated apatite. The carbonate content of apatite was calibrated to a carbonate Raman band, and the method was used to determine the carbonate content of a sample of bovine cortical bone, 7.7 ± 0.4%.

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Acknowledgement

Our appreciation goes to Robert Buckland who prepared some of the apatite samples. This work was supported in part by NIH grant R01 AR47969.

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Authors and Affiliations

  1. Department of Chemistry, Central Michigan University, Mt. Pleasant, MI, 48859, USA

    Ayorinde Awonusi & Mary M. J. Tecklenburg

  2. Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA

    Michael D. Morris

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  1. Ayorinde Awonusi
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  2. Michael D. Morris
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  3. Mary M. J. Tecklenburg
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Correspondence to Mary M. J. Tecklenburg.

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Awonusi, A., Morris, M.D. & Tecklenburg, M.M.J. Carbonate Assignment and Calibration in the Raman Spectrum of Apatite. Calcif Tissue Int 81, 46–52 (2007). https://doi.org/10.1007/s00223-007-9034-0

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  • DOI: https://doi.org/10.1007/s00223-007-9034-0

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