Calcified Tissue International

, Volume 77, Issue 6, pp 339–347 | Cite as

Effects of the Cdx-2 Polymorphism of the Vitamin D Receptor Gene and Lifestyle Factors on Bone Mineral Density in a Representative Sample of Japanese Women: The Japanese Population-based Osteoporosis (JPOS) Study

  • A. Morita
  • M. Iki
  • Y. Dohi
  • Y. Ikeda
  • S. Kagamimori
  • Y. Kagawa
  • H. Yoneshima
Clinical Investigations

Abstract

Using a large-scale representative sample of the Japanese female population, we examined the effects of a single nucleotide polymorphism within a binding site of Cdx-2 in the promoter region of the vitamin D receptor gene on bone mineral density (BMD), and the interactions between this polymorphism and lifestyle factors on BMD. Fifty women were randomly selected from each of the 5-year age-stratified populations (15–79 years) in each of three chosen municipalities as a part of the Japanese Population-based Osteoporosis Study. BMD at the lumbar spine, hip, and distal forearm was measured using dual-energy X-ray absorptiometry at baseline and again in a follow-up study conducted 3 years later. Information on lifestyle factors was collected in a questionnaire and followed up in interviews. The G-to-A polymorphism within the Cdx-2 binding site was determined by a TaqMan allelic discrimination assay. At baseline, 1,340 women were analyzed. The baseline BMD in the ultradistal forearm in premenopausal women with the GG genotype was significantly lower than in those with other genotypes. There was no association between the Cdx-2 genotype and the change in BMD at any of the skeletal sites. We found significant associations between daily milk consumption and baseline BMD at some skeletal sites but only in subjects with the GG genotype. In conclusion, the Cdx-2 polymorphism alone did not have a substantial effect on BMD in Japanese women. However, this polymorphism might have some effect in women with low calcium intake.

Keywords

Cdx-2 polymorphism Lifestyle factor Bone mineral density Japanese women Representative sample 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • A. Morita
    • 1
  • M. Iki
    • 1
  • Y. Dohi
    • 2
  • Y. Ikeda
    • 1
  • S. Kagamimori
    • 3
  • Y. Kagawa
    • 4
  • H. Yoneshima
    • 5
  1. 1.Department of Public HealthKinki University School of MedicineOsaka-SayamaJapan
  2. 2.Department of Public HealthNara Medical UniversityKashiharaJapan
  3. 3.Department of Welfare Promotion and EpidemiologyToyama Medical and Pharmaceutical UniversityToyamaJapan
  4. 4.Kagawa Nutrition UniversityTokyoJapan
  5. 5.Kasukabe Shuuwa HospitalKasukabeJapan

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