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Diffusion Systems for Evaluation of Biomineralization

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Abstract

A variety of in vitro study methods have been used to elucidate the roles of matrix molecules in biomineralization processes. Among these, gel diffusion-precipitation studies have proved to be an effective tool. This methodology is uniquely capable of characterizing the effects of matrix molecules on mineralization while only using very small quantities of material. Furthermore, gel methods have been extended for use as a mineralization assay system to characterize modified matrix molecules and synthetic analogues. Here we discuss the advantages and limitations of gelatin, agar, agarose, and other systems for studying the mechanisms of biomineralization.

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References

  1. AL Boskey M Maresca W Ullrich SB Doty WT Butler CW Prince (1993) ArticleTitleOsteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel Bone Miner 22 147–159 Occurrence Handle1:CAS:528:DyaK2cXnsVagtQ%3D%3D Occurrence Handle8251766

    CAS  PubMed  Google Scholar 

  2. AL Boskey (1995) ArticleTitleOsteopontin and related phosphorylated sialoproteins: effects on mineralization Ann NY Acad Sci 760 249–256 Occurrence Handle1:CAS:528:DyaK2MXovVSitrY%3D Occurrence Handle7785899

    CAS  PubMed  Google Scholar 

  3. GK Hunter A Goldberg (1993) ArticleTitleNucleation of hydroxapatite by bone sialoprotein Proc Natl Acad Sci USA 90 8562–8565

    Google Scholar 

  4. GK Hunter HA Goldberg (1995) ArticleTitleThe inhibitory activity of osteopontin on hydroxyapatite formation in vitro Ann NY Acad Sci 760 305–308 Occurrence Handle7785906

    PubMed  Google Scholar 

  5. JD Termine K Kleinman SW Whitson KM Conn ML McGarvey GR Martin (1981) ArticleTitleOsteonectin, a bone-specific protein linking mineral to collagen Cell 26 99–105 Occurrence Handle10.1016/0092-8674(81)90037-4 Occurrence Handle1:CAS:528:DyaL38Xhs1amtQ%3D%3D Occurrence Handle7034958

    Article  CAS  PubMed  Google Scholar 

  6. GK Hunter PV Hauschka AR Poole LC Rosenberg HA Goldberg (1996) ArticleTitleNucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins Biochem J 317 59–64 Occurrence Handle1:CAS:528:DyaK28Xktl2mtb8%3D Occurrence Handle8694787

    CAS  PubMed  Google Scholar 

  7. GK Hunter HA Curtis MD Grynpas JP Simmer AG Fincham (1999) ArticleTitleEffects of recombinant amelogenin on hydroxyapatite formation in vitro Calcif Tissue Int 65 226–231 Occurrence Handle10.1007/s002239900688 Occurrence Handle1:CAS:528:DyaK1MXlt1SjsbY%3D Occurrence Handle10441656

    Article  CAS  PubMed  Google Scholar 

  8. M Iijima Y Moriwaki HB Wen AG Fincham J Moradian-Oldak (2002) ArticleTitleElongated growth of octacalcium phosphate crystals in recombinant amelogenin gels under controlled ionic flow J Dent Res 81 69–73 Occurrence Handle1:CAS:528:DC%2BD38XnvFemtA%3D%3D Occurrence Handle11820371

    CAS  PubMed  Google Scholar 

  9. CE Tye KR Rattra KJ Warner JA Gordon J Sodek GK Hunter HA Goldberg (2003) ArticleTitleDelineation of the hydroxyapatite-nucleating domains of bone sialoprotein J Biol Chem 278 7949–7955

    Google Scholar 

  10. JL Arias DJ Fink SQ Xiao AH Heuer AI Caplan (1993) ArticleTitleBiomineralization and eggshells: cell-mediated acellular compartments of mineralized extracellular matrix Int Rev Cytol 145 217–250

    Google Scholar 

  11. BA Gotliv L Addadi S Weiner (2003) ArticleTitleMollusk shell acidic proteins: in search of individual functions Chembiochem 4 522–529 Occurrence Handle10.1002/cbic.200200548 Occurrence Handle1:CAS:528:DC%2BD3sXkslCiuro%3D Occurrence Handle12794863

    Article  CAS  PubMed  Google Scholar 

  12. AL Boskey (1996) ArticleTitleMatrix proteins and mineralization Connect Tissue Res 35 357–363 Occurrence Handle1:CAS:528:DyaK2sXhvFamtbw%3D Occurrence Handle9084675

    CAS  PubMed  Google Scholar 

  13. HK Henisch (1988) Crystals in gels and liesegang rings Cambridge University Press Cambridge

    Google Scholar 

  14. NC Blumenthal AS Posner LD Silverman LC Rosenberg (1979) ArticleTitleEffect of proteoglycans on in vitro hydroxyapatite formation Calcif Tissue Int 27 75–82 Occurrence Handle1:CAS:528:DyaE1MXitVWnt78%3D Occurrence Handle111791

    CAS  PubMed  Google Scholar 

  15. Z Amjad P Koutsoukos MB Tomson GH Nancollas (1978) ArticleTitleThe growth of hydroxyapatite from solution. A new constant composition method J Dent Res 57 909 Occurrence Handle1:CAS:528:DyaE1MXltFSmsQ%3D%3D Occurrence Handle281366

    CAS  PubMed  Google Scholar 

  16. AL Boskey (1989) ArticleTitleHydroxyapatite formation in a dynamic collagen gel system: effects of type I collagen, lipids, and proteoglycans J Phys Chem 93 1628–1633 Occurrence Handle1:CAS:528:DyaL1MXptFKjtQ%3D%3D

    CAS  Google Scholar 

  17. GS Mandel PB Halverson NS Mandel (1988) ArticleTitleCalcium pyrophosphate crystal deposition: the effect of monosodium urate and apatite crystals in a kinetic study using a gelatin matrix model Scanning Microsc 2 1189–1198 Occurrence Handle1:CAS:528:DyaL1cXktlKju7w%3D Occurrence Handle2840735

    CAS  PubMed  Google Scholar 

  18. NS Mandel GS Mandel DJ Carroll PB Halverson (1984) ArticleTitleCalcium pyrophosphate crystal deposition. An in vitro study using a gelatin matrix model Arthritis Rheum 27 789–796 Occurrence Handle1:CAS:528:DyaL2cXlt1ygurw%3D Occurrence Handle6331461

    CAS  PubMed  Google Scholar 

  19. Z Pucar B Pokric A Graovac (1974) ArticleTitlePrecipitation in gels under conditions of double diffusion: critical concentrations of the precipitating components Anal Chem 46 403–407 Occurrence Handle1:CAS:528:DyaE2cXhtVSmurs%3D

    CAS  Google Scholar 

  20. AL Boskey M Maresca S Doty B Sabsay A Veis (1990) ArticleTitleConcentration-dependent effects of dentin phosphophoryn in the regulation of in vitro hydroxyapatite formation and growth Bone Miner 11 55–65 Occurrence Handle10.1016/0169-6009(90)90015-8 Occurrence Handle1:CAS:528:DyaK3MXpvVygtA%3D%3D Occurrence Handle2176557

    Article  CAS  PubMed  Google Scholar 

  21. AL Boskey L Spevak SB Doty L Rosenberg (1997) ArticleTitleEffects of bone CS-proteoglycans, DS-decorin, and DS-biglycan on hydroxyapatite formation in gelatin gel Calcif Tissue Int 61 298–305 Occurrence Handle10.1007/s002239900339 Occurrence Handle1:CAS:528:DyaK2sXmsFCltbw%3D Occurrence Handle9312200

    Article  CAS  PubMed  Google Scholar 

  22. AL Boskey L Spevak M Tan SB Doty WT Butler (2000) ArticleTitleDentin sialoprotein (DSP) has limited effects on in vitro apatite formation and growth Calcif Tissue Int 67 472–478 Occurrence Handle10.1007/s002230001169 Occurrence Handle1:CAS:528:DC%2BD3MXhvVOruro%3D Occurrence Handle11289697

    Article  CAS  PubMed  Google Scholar 

  23. PH Tartaix M Doulaverakis A George et al. (2004) ArticleTitleIn vitro effects of dentin matrix protein-1 on hydroxyapatite formation provide insights into in vivo functions J Biol Chem 279 18115–18120

    Google Scholar 

  24. AL Boskey BD Boyan Z Schwartz (1997) ArticleTitleMatrix vesicles promote mineralization in a gelatin gel system Calcif Tissue Int 60 309–315 Occurrence Handle10.1007/s002239900234 Occurrence Handle1:CAS:528:DyaK2sXhsFKgtLY%3D Occurrence Handle9069171

    Article  CAS  PubMed  Google Scholar 

  25. EM Greenfield DC Wilson MA Crenshaw (1984) ArticleTitleIonotropic nucleation of calcium carbonate by molluscan matrix Am Zool 24 925–932 Occurrence Handle1:CAS:528:DyaL2MXptleisA%3D%3D

    CAS  Google Scholar 

  26. L Addadi S Weiner M Geva (2001) ArticleTitleOn how proteins interact with crystals and their effect on crystal formation Z Kardiol 90 IssueIDsuppl 3 392–398 Occurrence Handle10.1007/s003920170049

    Article  Google Scholar 

  27. L Addadi S Weiner (1985) ArticleTitleInteractions between acidic proteins and crystals: stereochemical requirements in biomineralization Proc Natl Acad Sci USA 88 4110–4114

    Google Scholar 

  28. GK Hunter CL Kyle HA Goldberg (1994) ArticleTitleModulation of crystal formation by bone phosphoproteins: structural specificity of the osteopontin-mediated inhibition of hydroxyapatite formation Biochem J 300 723–728 Occurrence Handle1:CAS:528:DyaK2cXkt12mt78%3D Occurrence Handle8010953

    CAS  PubMed  Google Scholar 

  29. HA Goldberg KJ Warner MJ Stillman GK Hunter (1996) ArticleTitleDetermination of the hydroxyapatite-nucleating region of bone sialoprotein Connect Tissue Res 35 385–392 Occurrence Handle1:CAS:528:DyaK2sXhvFals70%3D Occurrence Handle9084679

    CAS  PubMed  Google Scholar 

  30. GK Hunter HA Goldberg (1994) ArticleTitleModulation of crystal formation by bone phosphoproteins: role for glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein Biochem J 302 175–179 Occurrence Handle1:CAS:528:DyaK2cXltlSktrw%3D Occurrence Handle7915111

    CAS  PubMed  Google Scholar 

  31. NL Harris KR Rattray CE Tye TM Underhill MJ Somerman JA D’Errico AI Chambers GK Hunter FA Goldberg (2000) ArticleTitleFunctional analysis of bone sialoprotein: identification of the hydroxyapatite-nucleating and cell-binding domains by recombhiant peptide expression and site-directed mutagenesis Bone 27 795–802 Occurrence Handle10.1016/S8756-3282(00)00392-6 Occurrence Handle1:CAS:528:DC%2BD3cXosFCisbs%3D Occurrence Handle11113390

    Article  CAS  PubMed  Google Scholar 

  32. Y Wada R Fujisawa Y Nodasaka Y Kuboki (1996) ArticleTitleElectrophoretic gels of dentin matrix proteins as diffusion media for in vitro mineralization J Dent Res 75 1381–1387 Occurrence Handle1:CAS:528:DyaK28XmsVOqtbo%3D Occurrence Handle8831633

    CAS  PubMed  Google Scholar 

  33. R Fujisawa Y Kuboki S Sasaki (1987) ArticleTitleEffects of dentin phosphophoryn on precipitation of calcium phosphate in gel in vitro Calcif Tissue Int 41 44–47

    Google Scholar 

  34. AL Boskey M Maresca J Appel (1989) ArticleTitleThe effects of noncollageneous matrix proteins on hydroxyapatite formation and proliferation in a collagen gel system Connect Tissue Res 21 171–178 Occurrence Handle1:STN:280:By%2BC3cjktlY%3D Occurrence Handle2605941

    CAS  PubMed  Google Scholar 

  35. R Fujisawa Y Wada Y Nodasaka Y Kuboki (1996) ArticleTitleAcidic amino acid-rich sequences as binding sites of osteonectin to hydroxyapatite crystals Biochim Biophys Acta 1292 53–60 Occurrence Handle10.1016/0167-4838(95)00190-5 Occurrence Handle1:CAS:528:DyaK28Xhsl2ksA%3D%3D Occurrence Handle8547349

    Article  CAS  PubMed  Google Scholar 

  36. D Couchourel C Escoffier R Rohanizadeh S Bohic G Daculsi Y Fortun M Padrines (1999) ArticleTitleEffects of fibronectin on hydroxyapatite formation J Inorg Biochem 73 129–136 Occurrence Handle10.1016/S0162-0134(99)00006-9 Occurrence Handle1:CAS:528:DyaK1MXjtFChtr0%3D Occurrence Handle10331242

    Article  CAS  PubMed  Google Scholar 

  37. T Taira M Iijima Y Moriwaki Y Kuboki (1995) ArticleTitleA new method for in vitro calcification using acrylamide gel and bovine serum Connect Tissue Res 33 185–192 Occurrence Handle1:STN:280:BymD3s3htlQ%3D Occurrence Handle7554952

    CAS  PubMed  Google Scholar 

  38. N Bouropoulos J Moradian-Oldak (2004) ArticleTitleInduction of apatite by the cooperative effect of amelogenin and the 32-kDa enamelin J Dent Res 83 278–282 Occurrence Handle1:CAS:528:DC%2BD2cXjs1ClurY%3D Occurrence Handle15044499

    CAS  PubMed  Google Scholar 

  39. M Iijima Y Moriwaki T Takagi J Moradian-Oldak (2001) ArticleTitleEffects of bovine amelogenins on the crystal morphology of octacalcium phosphate in a model system of tooth enamel formation J Cryst Growth 222 615–626 Occurrence Handle10.1016/S0022-0248(00)00984-2 Occurrence Handle1:CAS:528:DC%2BD3MXksFKjuw%3D%3D

    Article  CAS  Google Scholar 

  40. HB Wen J Moradian-Oldak AG Fincham (2000) ArticleTitleDose-dependent modulation of octacalcium phosphate crystal habit by ameloginins J Dent Res 79 1902–1906 Occurrence Handle1:CAS:528:DC%2BD3MXktFKmtQ%3D%3D Occurrence Handle11145363

    CAS  PubMed  Google Scholar 

  41. G He T Dahl A Veis A Geogre (2003) ArticleTitleNucleation of apatite crystals in vitro by self-assembled dentin matrix protein-1 Nat Mater 2 552–558 Occurrence Handle10.1038/nmat945 Occurrence Handle1:CAS:528:DC%2BD3sXlvFaku78%3D Occurrence Handle12872163

    Article  CAS  PubMed  Google Scholar 

  42. D Srzic B Pokric Z Pucar (1976) ArticleTitlePrecipitation in gels under conditions of double diffusion: critical concentrations and solubility products of salts Z Physi Chem 103 157–164 Occurrence Handle1:CAS:528:DyaE2sXht1OrtLg%3D

    CAS  Google Scholar 

  43. B Pokric Z Pucar (1979) ArticleTitlePrecipitation of calcium phosphate under conditions of double diffusion in collagen and gels of gelatin and agar Calcif Tissue Int 27 171–176 Occurrence Handle1:CAS:528:DyaE1MXkvVynsLk%3D Occurrence Handle110419

    CAS  PubMed  Google Scholar 

  44. P Somasundaran YHC Wang (1984) Surface chemical characteristics and adsorption properties of apatite DN Misra (Eds) Adsorption on and surface chemistry of hydroxyapatite Plenum Press New York 129

    Google Scholar 

  45. DT Hughes Wassell RC Hall G Embery (1995) ArticleTitleAdsorption of bovine serum albumin onto hydroxyapatite Biomaterials 16 697–702 Occurrence Handle10.1016/0142-9612(95)99697-K Occurrence Handle7578773

    Article  PubMed  Google Scholar 

  46. GK Hunter SC Nyburg KPH Pritzker (1986) ArticleTitleHydroxyapatite formation in collagen, gelatin, and agarose gels Coll Rel Res 61 229–238

    Google Scholar 

  47. S Eiden-Abmann M Viertelhaus A Heiß KA Hoetzer J Felsche (2002) ArticleTitleThe influence of amino acids on the biomineralization of hydroxyapatite in gelatin J Inorg Biochem 91 481–486 Occurrence Handle10.1016/S0162-0134(02)00481-6 Occurrence Handle12175941

    Article  PubMed  Google Scholar 

  48. R Fujisawa Y Nodasake Y Kuboki (1995) ArticleTitleFurther characterization of interaction between bone sialoprotein (BSP) and collagen Calcif Tissue Int 56 140–144 Occurrence Handle1:CAS:528:DyaK2MXkvVylu7c%3D Occurrence Handle7736323

    CAS  PubMed  Google Scholar 

  49. T Saito M Yamauchi MA Crenshaw (1998) ArticleTitleApatite induction by insoluble dentin collagen J Bone Miner Res 13 265–270 Occurrence Handle1:CAS:528:DyaK1cXhtlSltr8%3D Occurrence Handle9495520

    CAS  PubMed  Google Scholar 

  50. T Saito AL Arsenault M Yamauchi Y Kuboki MA Crenshaw (1997) ArticleTitleMineral induction by immobilized phosphoproteins Bone 21 305–311 Occurrence Handle10.1016/S8756-3282(97)00149-X Occurrence Handle1:CAS:528:DyaK2sXnt1Klt74%3D Occurrence Handle9315333

    Article  CAS  PubMed  Google Scholar 

  51. A Linde A Lussi MA Crenshaw (1989) ArticleTitleMineral induction by immobilized polyanionic proteins Calcif Tissue Int 44 286–295 Occurrence Handle1:CAS:528:DyaL1MXktVakur8%3D Occurrence Handle2501010

    CAS  PubMed  Google Scholar 

  52. A Lussi MA Crenshaw A Linde (1988) ArticleTitleInduction and inhibition of hydroxyapatite formation by rat dentine phosphoprotein in vitro Arch oral Biol 33 685–691 Occurrence Handle10.1016/0003-9969(88)90124-0 Occurrence Handle1:CAS:528:DyaL1cXmt1Onuro%3D Occurrence Handle3245795

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors wish to acknowledge the help of Melin Tan, Hospital for Special Surgery, and Aryeh Keehn, Yeshiva University, for their contributions exploring single diffusion gelatin systems. Dr Boskey’s work discussed in this paper was supported by NIH grant DE04141.

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Authors and Affiliations

  1. Yeshiva University, 500 West 185 St., New York, NY, 10033, USA

    L. Silverman

  2. Starr Chair in Mineralized Tissue Research, Hospital for Special Surgery, affiliated with Weill Medical College of Cornell University, New York, NY, 10021, USA

    A. L. Boskey

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  1. L. Silverman
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Correspondence to A. L. Boskey.

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Silverman, L., Boskey, A.L. Diffusion Systems for Evaluation of Biomineralization. Calcif Tissue Int 75, 494–501 (2004). https://doi.org/10.1007/s00223-004-0019-y

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