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Morphological Changes in Diseased Cementum Layers: A Scanning Electron Microscopy Study

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Abstract

The aim of this study was to compare the morphological changes that occurred in root cementum layers due to periodontal disease by using scanning electron microscopy (SEM). Ninety-two periodontally hopeless teeth extracted from 29 patients were studied. Measurements of probing depth (PD) and clinical attachment loss (CAL) were taken prior to extractions. After the longitudinal fracturing process of root specimens, healthy and diseased cementum layers of roots were evaluated by SEM for the thickness of the cementum and the morphological changes in collagen fibers. The result of SEM evaluation revealed a significant (P < 0.001) decrease in the thickness of cementum layer on the diseased root surfaces compared to the healthy surfaces. There were denser and conspicuous collagen fibers with their interfibrillar matrix in cementum layers on the healthy root surfaces compared to the diseased surfaces. Within the limits of this study, the thickness of cementum layers in diseased areas was found to be significantly less than that in the healthy areas of root surfaces. However, there exist variations in the density and visibility of cemental fibers between individuals and within the individual.

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Acknowledgements

This work was supported by a grant from Ankara University Research Foundation (No. 20010802038). The authors would like to acknowledge the statistical assistance provided by Dr. Atilla Elhan and express our appreciation to Defne Akinci for her assistance in preparing this manuscript in English.

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Authors and Affiliations

  1. Ankara University, Faculty of Dentistry, Department of Periodontology, Ankara, Turkey

    E. Bilgin, C. A. Gürgan, M. Nejat Arpak & H. S. Bostanci

  2. Kirikkale University, Faculty of Art and Science, Department of Physics, Ankara, Turkey

    K. Güven

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  1. E. Bilgin
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  2. C. A. Gürgan
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  3. M. Nejat Arpak
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  4. H. S. Bostanci
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  5. K. Güven
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Correspondence to C. A. Gürgan.

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Bilgin, E., Gürgan, C.A., Arpak, M.N. et al. Morphological Changes in Diseased Cementum Layers: A Scanning Electron Microscopy Study. Calcif Tissue Int 74, 476–485 (2004). https://doi.org/10.1007/s00223-003-0195-1

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  • DOI: https://doi.org/10.1007/s00223-003-0195-1

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