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Weak Evidence of Thyrotropin Receptors in Primary Cultures of Human Osteoblast-like Cells

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Abstract

Extra-thyroidal thyrotropin (TSH) receptors (TSHRs) have been demonstrated in several tissues and cells, including human and rat osteosarcoma cell lines. We have explored whether human TSHR (hTSHRs) also are present in primary cultures of human osteoblast-like (hOB) cells. [125 I]TSH binding was limited in hOB cells, but somewhat higher in UMR 106-01 cells and considerably higher in hTSHR-transfected CHO cells. In hOB cells, the basal intracellular cAMP levels increased 282% after stimulation with 10 U/L TSH. In the hTSHR-transfected CHO cells, the cAMP increase was 3030% in response to 10 U/L TSH and 1240% after 1 U/L TSH. Free cytoplasmic calcium did not change in response to TSH in hOB cells. HTSHR mRNA was detected in hOB cells from 3/4 bone by reverse transcriptase polymerase chain reaction RT-PCR and nucleotide sequencing HTSHR mRNA, but could not be demonstrated with the RNase protection technique in hOB cells from 5 different donors. In conclusion, even after the use of several methods, we have found only weak evidence for expression and presence of functionally active hTSHR in hOB cells. Given the low level of expression, specific binding and cAMP signaling, we suggest that it is unlikely that circulating TSH plays a physiological role for bone metabolism mediated through osteoblasts.

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Acknowledgments

We thank Ms. Ulrika Sjöstedt and Ms. Barbro Granberg for excellent technical assistance and Professor J. Morris at the Mayo Clinic, Rochester, MN, USA, for donation of pTSH. This work was supported by the Swedish Medical Research Council (5992 & 9941) and the Funds of Karolinska Institutet and Cancer och Trafikskadades Riksförbund. The authors are also grateful to Associate Professors Li Felländer-Tsai and Leif Perbeck, Huddinge University Hospital, for supplying bone material and thyroid tissue.

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Authors and Affiliations

  1. Department of Surgery, Huddinge University Hospital, P Karolinska Institutet, Stockholm, Sweden

    J. A. Tsai

  2. Department of Pediatrics, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden

    A. Janson, C. Marcus & H. Rawet Zemack

  3. Department of Molecular Medicine, Karolinska Hospital, Karolinska Institutet, Stockholm, Sweden

    E. Bucht

  4. Department of Medical Sciences, Uppsala Academic Hospital, Uppsala University, Sweden

    H. Kindmark

  5. Department of Orthopedic Surgery, Karolinska Institutet, Stockholm, Sweden

    A. Stark

  6. Department of Medicine, Sodersjukhuset, Karolinska Institutet, Stockholm, Sweden

    O. Torring

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  1. J. A. Tsai
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  2. A. Janson
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  3. E. Bucht
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Correspondence to O. Torring.

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Tsai, J.A., Janson, A., Bucht, E. et al. Weak Evidence of Thyrotropin Receptors in Primary Cultures of Human Osteoblast-like Cells. Calcif Tissue Int 74, 486–491 (2004). https://doi.org/10.1007/s00223-003-0108-3

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  • DOI: https://doi.org/10.1007/s00223-003-0108-3

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