Skip to main content

Advertisement

SpringerLink
Log in

Effect of Pamidronate on Excretion of Pyridinium Crosslinks of Collagen After Total Hip Arthroplasty

  • Clinical Investigation
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Abstract

Periprosthetic bone loss is an important factor that limits implant survival after total hip arthroplasty (THA). In a randomized trial we previously reported that pamidronate therapy prevented periprosthetic bone loss and decreased urinary excretion of N-telopeptide collagen cross-links over the first 6 months after THA, but had no apparent effect on free deoxypyridinoline excretion (J Bone Miner Res 2001; 16:556–564). In this study we investigated this discrepant observation that pamidronate reduced conjugated cross-link excretion but had no effect on free cross-links. Free and total deoxypyridinoline (DPD) were assayed by reverse-phase high-performance liquid chromatography (HPLC) and by immunosorbent assay (ELISA) at preoperative baseline and at week 6 after surgery in 46 subjects who had taken part in the trial. Randomly selected, 22 subjects received a single 90 mg intravenous infusion of pamidronate and 24 received placebo. Acute rises in free and total DPD occurred in both study groups at week 6 (P < 0.05). Total DPD excretion was lower in the pamidronate group than in the placebo group when measured by both HPLC and ELISA (P < 0.05). No difference in free DPD was found between groups. A rise in the ratio of free to total DPD occurred in the pamidronate group at week 6 (P = 0.03), but not in the placebo group. Pamidronate treatment suppresses excretion of total DPD. This is consistent with the effect of pamidronate on other turnover markers and periprosthetic bone loss after THA. Urinary-free DPD is a poor marker of response to treatment as the ratio of free-to-total cross-links is affected by amino-bisphosphonate therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  1. Z Gunja-Smith RJ Boucek (1981) ArticleTitleCollagen cross-linking compounds in human urine. Biochem J 197 759–762

    Google Scholar 

  2. R Eastell A Colwell L Hampton J Reeve (1997) ArticleTitleBiochemical markers of bone resorption compared with estimates of bone resorption from radiotracer kinetic studies in osteoporosis. J Bone Miner Res 12 59–65 Occurrence Handle1:STN:280:ByiA1c3nsVc%3D Occurrence Handle9240726

    CAS  PubMed  Google Scholar 

  3. R Eastell PD Delmas SF Hodgson EF Eriksen KG Mann BL Riggs (1988) ArticleTitleBone formation rate in older normal women: concurrent assessment with bone histomorphometry, calcium kinetics, and biochemical markers. J Clin Endocrinol Metab 67 741–748 Occurrence Handle1:CAS:528:DyaL1cXlvVOrurc%3D Occurrence Handle3262119

    CAS  PubMed  Google Scholar 

  4. P Garnero E Sornay-Rendu B Claustrat PD Delmas (2000) ArticleTitleBiochemical markers of bone turnover, endogenous hormones and the risk of fractures in postmenopausal women: the OFELY study. J Bone Miner Res 15 1526–1536 Occurrence Handle1:CAS:528:DC%2BD3cXlvVSjtrg%3D Occurrence Handle10934651

    CAS  PubMed  Google Scholar 

  5. CJ Rosen CH Chesnut NJ Mallinak (1997) ArticleTitleThe predictive value of biochemical markers of bone turnover for bone mineral density in early post-menopausal women treated with hormone replacement or calcium supplementation. J Clin Endocrinol Metab 82 1904–1910 Occurrence Handle1:CAS:528:DyaK2sXjs1ent7Y%3D Occurrence Handle9177404

    CAS  PubMed  Google Scholar 

  6. PD Ross W Knowlton (1998) ArticleTitleRapid bone loss is associated with increased levels of biochemical markers. J Bone Miner Res 13 297–302 Occurrence Handle1:STN:280:DyaK1c7lsV2isA%3D%3D Occurrence Handle9495524

    CAS  PubMed  Google Scholar 

  7. de Braga MA Castro R Hannon R Eastell (1999) ArticleTitleMonitoring alendronate therapy for osteoporosis. J Bone Miner Res 14 602–608 Occurrence Handle10234582

    PubMed  Google Scholar 

  8. DC Bauer PM Sklarin KL Stone DM Black MC Nevitt KE Ensrud CD Arnaud HK Genant P Garnero PD Delmas H Lawaetz SR Cummings (1999) ArticleTitleBiochemical markers of bone turnover and prediction of hip bone loss in older women: the study of osteoporotic fractures. J Bone Miner Res 14 1404–1410 Occurrence Handle1:STN:280:DyaK1MzovVahsw%3D%3D Occurrence Handle10457273

    CAS  PubMed  Google Scholar 

  9. P Garnero E Hausherr MC Chapuy C Marcelli H Grandjean C Muller C Cormier G Breart PJ Meunier PD Delmas (1996) ArticleTitleMarkers of bone resorption predict hip fracture in elderly women: the EPIDOS Prospective Study. J Bone Miner Res 11 1531–1538 Occurrence Handle1:STN:280:ByiD2cvnvVc%3D Occurrence Handle8889854

    CAS  PubMed  Google Scholar 

  10. R Marcus L Holloway B Wells G Greendale MK James C Wasilauskas J Kelaghan (1999) ArticleTitleThe relationship of biochemical markers of bone turnover to bone density changes in postmenopausal women: results from the Postmenopausal Estrogen/Progestin Interventions (PEPI) trial. J Bone Miner Res 14 1583–1595 Occurrence Handle1:CAS:528:DyaK1MXmtVyqurs%3D Occurrence Handle10469288

    CAS  PubMed  Google Scholar 

  11. JD Bobyn ES Mortimer AH Glassmenn CA Engh JE Miller CE Brookes (1992) ArticleTitleProducing and avoiding stress shielding. Laboratory and clinical observations of noncemented total hip arthroplasty. Clin Orthop 274 79–96

    Google Scholar 

  12. HG Willert GH Bucchorn (1999) The biology of the loosening of hip implants. RP Jacob P Fulford F Horan (Eds) European Instructional Course Lectures. The British Editorial Society of Bone and Joint Surgery London 58–82

    Google Scholar 

  13. NJ Harris A Lowman L Wolsey R Hannon TWD Smith R Eastell (2000) ArticleTitleChanges in bone turnover and bone density after total hip arthroplasty. Hip Int 10 16–20

    Google Scholar 

  14. U Schneider SJ Breusch S Termath M Thomsen DRC Brocai F-U Niethard C Kasperk (1998) ArticleTitleIncreased urinary crosslink levels in aseptic loosening of total hip arthroplasty. J Arthroplasty 13 687–692 Occurrence Handle1:STN:280:DyaK1cvht1Ckug%3D%3D Occurrence Handle9741447

    CAS  PubMed  Google Scholar 

  15. JM Wilkinson AJ Hamer A Rogers I Stockley R Eastell (2003) ArticleTitleBone mineral density and biochemical markers of bone turnover in aseptic loosening after total hip arthroplasty. J Orthop Res 21 691–696 Occurrence Handle10.1016/S0736-0266(02)00237-1 Occurrence Handle1:CAS:528:DC%2BD3sXkt1OksbY%3D Occurrence Handle12798070

    Article  CAS  PubMed  Google Scholar 

  16. J Antoniou O Huk D Zukor D Eyre M Alini (2000) ArticleTitleCollagen crosslinked N-telopeptides as markers for evaluating particulate osteolysis: a preliminary study. J Orthop Res 18 64–67 Occurrence Handle1:CAS:528:DC%2BD3cXitVOitLg%3D Occurrence Handle10716280

    CAS  PubMed  Google Scholar 

  17. JM Wilkinson I Stockley NFA Peel AJ Hamer RA Elson NA Barrington R Eastell (2001) ArticleTitleEffect of pamidronate in preventing local bone loss after total hip arthroplasty: a randomized, double-blind, controlled trial. J Bone Miner Res 16 556–564 Occurrence Handle1:CAS:528:DC%2BD3MXhvFKktrc%3D Occurrence Handle11277274

    CAS  PubMed  Google Scholar 

  18. DR Eyre TJ Koob KP Van Ness (1984) ArticleTitleQuantitation of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography. Anal Biochem 137 380–388 Occurrence Handle1:CAS:528:DyaL2cXhvVyiu7g%3D Occurrence Handle6731820

    CAS  PubMed  Google Scholar 

  19. A Colwell R Eastell (1996) ArticleTitleThe renal clearance of free and conjugated pyridinium cross-links of collagen. J Bone Miner Res 11 1976–1980 Occurrence Handle1:CAS:528:DyaK2sXjtVCksg%3D%3D Occurrence Handle8970901

    CAS  PubMed  Google Scholar 

  20. SP Robins H Woitge R Hesley J Ju S Seyedin MJ Seibel (1994) ArticleTitleDirect, enzyme-linked immunoassay for urinary deoxypyridinoline as a specific marker for measuring bone resorption. J Bone Miner Res 9 1643–1649 Occurrence Handle1:CAS:528:DyaK2cXmvFKmsr0%3D Occurrence Handle7817812

    CAS  PubMed  Google Scholar 

  21. BJ Gertz P Shao DA Hanson H Quan ST Harris HK Genant CH Chesnut III DR Eyre (1994) ArticleTitleMonitoring bone resorption in early postmenopausal women by an immunoassay for cross-linked collagen peptides in urine. J Bone Miner Res 9 135–142 Occurrence Handle1:CAS:528:DyaK2cXis1Git7o%3D Occurrence Handle8140926

    CAS  PubMed  Google Scholar 

  22. P Garnero E Gineyts P Arbault C Christiansen PD Delmas (1995) ArticleTitleDifferent effects of bisphosphonate and estrogen therapy on free and peptide-bound bone cross-links excretion. J Bone Miner Res 10 641–649 Occurrence Handle1:STN:280:ByqA3srjtFE%3D Occurrence Handle7610936

    CAS  PubMed  Google Scholar 

  23. JH Tobias CV Laversuch N Wilson SP Robins (1996) ArticleTitleNeridronate preferentially suppresses the urinary excretion of peptide-bound deoxypyridinoline in postmenopausal women. Calcif Tissue Int 59 407–409 Occurrence Handle10.1007/s002239900148 Occurrence Handle1:CAS:528:DyaK28Xms1Kgsbs%3D Occurrence Handle8849410

    Article  CAS  PubMed  Google Scholar 

  24. GJ Rucklidge GI Riddoch LM Williams SP Robins (1988) ArticleTitleAutoradiographic studies of the renal clearance of circulating type I collagen fragments in the rat. Collagen & Related Research 8 339–348

    Google Scholar 

Download references

Acknowledgements

We wish to acknowledge grant support from the Wishbone Trust, The Royal College of Surgeons of England, and The John Charnley Trust.

Author information

Authors and Affiliations

  1. Bone Metabolism Group, Division of Clinical Sciences (North), University of Sheffield, Northern General Hospital, Herries Road, Sheffield, S5 7AU, UK

    J. Mark Wilkinson, Brendan Jackson & Richard Eastell

Authors
  1. J. Mark Wilkinson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brendan Jackson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard Eastell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Mark Wilkinson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mark Wilkinson, J., Jackson, B. & Eastell, R. Effect of Pamidronate on Excretion of Pyridinium Crosslinks of Collagen After Total Hip Arthroplasty . Calcif Tissue Int 73, 326–331 (2003). https://doi.org/10.1007/s00223-002-2154-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00223-002-2154-7

Keywords

Search

Navigation