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Congenital Lack of COX-2 Affects Mechanical and Geometric Properties of Bone in Mice

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Abstract

We compared the mechanical properties of bones from mice lacking either a functional cycloxygenase-1 (C57BL6/DBA COX-1−/−; n = 9) or COX-2 (C57BL6/DBA COX-2−/−; n = 9) gene and wild type mice (C57BL6/DBA; n = 10). Twenty-eight right femora from 3-month-old male mice were used to determine bulk structural and material properties of bone by three-point bending. Bone matrix properties were also measured by nanoindentation to access the changes in bulk mechanical properties due to changes in bone matrix or bone geometry. The bulk material properties (elastic modulus, P < 0.05; ultimate stress, P < 0.01) of COX-2−/− bones were lower than those of wild-type mice whereas the bulk structural properties (stiffness, P > 0.2; breaking force, P > 0.1) were similar to those of the wild-type mice. COX-2−/− mice had a longer moment of inertia but their cortical bones were thinner and contained many more intra-cortical pores compared with the bones of the other two groups. Finally, the bone matrix properties of COX-1−/− mice, COX-2−/− mice and their heterozygous littermates were similar to those of C57BL6/DBA wild-type mice.

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Acknowledgements

These studies were supported by grants from the Department of Veterans Affairs (DVA) and the National Institutes of Health (RR), and the Arthritis Foundation (JR). Rajendra Raghow is a Senior Research Career Scientist of DVA.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee, USA

    Q. Chen, J. Y. Rho & Z. Fan

  2. Department of Veterans Affairs Medical Center, Memphis, Tennessee, USA

    S. J. F. Laulederkind & R. Raghow

  3. Departments of Pharmacology and Biomedical Engineering, University of Tennessee Health Science Center, 1030 Jefferson Avenue, Memphis, Tennessee 38104, USA

    R. Raghow

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  1. Q. Chen
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Correspondence to R. Raghow.

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Chen, Q., Rho, J., Fan, Z. et al. Congenital Lack of COX-2 Affects Mechanical and Geometric Properties of Bone in Mice . Calcif Tissue Int 73, 387–392 (2003). https://doi.org/10.1007/s00223-002-0009-x

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  • DOI: https://doi.org/10.1007/s00223-002-0009-x

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