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Experimental Brain Research

, Volume 225, Issue 2, pp 205–215 | Cite as

Using vertebral movement and intact paraspinal muscles to determine the distribution of intrafusal fiber innervation of muscle spindle afferents in the anesthetized cat

  • William R. ReedEmail author
  • Dong-Yuan Cao
  • Weiqing Ge
  • Joel G. Pickar
Research Article

Abstract

Increasing our knowledge regarding intrafusal fiber distribution and physiology of paraspinal proprioceptors may provide key insights regarding proprioceptive deficits in trunk control associated with low back pain and lead to more effective clinical intervention. The use of vertebral movement as a means to reliably stretch paraspinal muscles would greatly facilitate physiological study of paraspinal muscle proprioceptors where muscle tendon isolation is either very difficult or impossible. The effects of succinylcholine (SCh) on 194 muscle spindle afferents from lumbar longissimus or multifidus muscles in response to computer-controlled, ramp-and-hold movements of the L6 vertebra were investigated in anesthetized cats. Paraspinal muscles were stretched by moving the L6 vertebra 1.5–1.7 mm in the dorsal–ventral direction. Initial frequency (IF), dynamic difference (DD), their changes (∆) following SCh injection (100–400 μg kg−1), and post-SCh dynamic difference (SChDD) were measured. Muscle spindle intrafusal fiber terminations were classified as primary or secondary fibers as well as bag1 (b1c), bag2 (b2c), b1b2c, or chain (c) fibers. Intrafusal fiber subpopulations were distinguished using logarithmic transformation of SChDD and ∆IF distributions as established by previous investigators. Increases in DD indicate strength of b1c influence while increases in IF indicate strength of b2c influence. Out of 194 afferents, 46.9 % of afferents terminated on b2c fibers, 46.4 % on b1b2c fibers, 1 % on b1c fibers, and 5.7 % terminated on c fibers. Based on these intrafusal fiber subpopulation distributions, controlled vertebral movement can effectively substitute for direct tendon stretch and allow further investigation of paraspinal proprioceptors in this anatomically complex body region.

Keywords

Muscle spindle Neurophysiology Paraspinal muscles Trunk Lumbar spine Succinylcholine 

Notes

Acknowledgments

The authors thank Randall Sozio for technical work, Dr. Cyndy Long for statistical consultation, and Ying Cao for statistical assistance. This work was supported by NIH grants: NIH U19 AT004137 and NS46818 to JGP; and K01AT005935 to WRR. It was conducted in a facility constructed with support from Research Facilities Improvement Grant Number C06 RR15433 from the National Center for Research Resources, NIH.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • William R. Reed
    • 1
    Email author
  • Dong-Yuan Cao
    • 1
    • 2
  • Weiqing Ge
    • 1
    • 3
  • Joel G. Pickar
    • 1
  1. 1.Palmer Center for Chiropractic ResearchDavenportUSA
  2. 2.Department of Neural and Pain SciencesUniversity of Maryland School of DentistryBaltimoreUSA
  3. 3.Department of Physical TherapyYoungstown State UniversityYoungstownUSA

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