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A near infrared spectroscopy study investigating oxygen utilisation in hydrocephalic rats

Experimental Brain Research volume 175, pages 127–138 (2006)Cite this article

Abstract

Determination of hydrocephalus and its severity is important for optimal management of the condition. We have used near infrared spectroscopy (NIRS) to assess changes in concentrations of oxygenated (O2Hb), deoxygenated (HHb), total haemoglobin (tHb) and cytochrome c oxidase (Caa3) in normal and hydrocephalic Texas (HTx) rats in response to a 5 min head down tilt and a sodium pentobarbitone (NaPB) challenge. The former was used to test vascular responses and the latter to test metabolic responses. The haemoglobin oxygenation index (HbD) was derived which provides information regarding oxygen utilisation ([HbD]=[O2Hb]−[HHb]). With the tilt challenge, a significant (P=0.001) difference was observed in [HbD] between normal (n=24) and hydrocephalic (n=14) rats (–3.50 (−6.00 to 0.00) μM cm−1 and 7.50 (0.75 to 14.25) μM cm−1, respectively). In another experiment we tested the response of ten rats to NaPB administration and observed a significant difference (P=0.008) in [Caa3] between normal (n=5) and hydrocephalic (n=5) rats (−6.60 (−7.55 to −5.50) μM cm−1 and −2.20 (−5.60 to −1.05) μM cm−1, respectively). Coronal sections of these ten rat brains were analysed and significant (P<0.05) relationships were found between some of the NIRS parameters and cortical thickness or lateral ventricle area measurements. Our studies demonstrate that a significant difference in cerebral oxygenation and haemodynamics can be observed between normal and hydrocephalic HTx rats using NIRS.

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Acknowledgements

We wish to thank Janet Wilson Walsh and Nick Ritchie for technical support and Simon Christie and Dame Carys M. Bannister OBE for helpful suggestions throughout the study. This work was funded by the EPSRC and The University of Manchester.

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Affiliations

  1. School of Chemical Engineering and Analytical Science, The University of Manchester, Sackville Street, PO Box 88, Manchester, M60 1QD, UK

    Zareen Bashir, Jemma Miller & Maureen Susan Thorniley

  2. Faculty of Life Sciences, The University of Manchester, Stopford Building, Oxford RD, M13 9PT, Manchester, UK

    Jaleel Ahmad Miyan

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  1. Zareen Bashir
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  2. Jemma Miller
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  3. Jaleel Ahmad Miyan
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  4. Maureen Susan Thorniley
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Correspondence to Zareen Bashir.

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Bashir, Z., Miller, J., Miyan, J.A. et al. A near infrared spectroscopy study investigating oxygen utilisation in hydrocephalic rats. Exp Brain Res 175, 127–138 (2006). https://doi.org/10.1007/s00221-006-0541-4

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Keywords

  • Cerebral haemodynamics and metabolism
  • Head down tilt
  • Hydrocephalus
  • Near infrared spectroscopy
  • Rats
  • Sodium pentobarbitone