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GABAA receptor subunit expression in the guinea pig vestibular nucleus complex during the development of vestibular compensation

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Abstract

The aim of this experiment was to investigate whether vestibular compensation following unilateral vestibular deafferentation (UVD) is associated with changes in the expression of GABAA receptor subunits in the guinea pig vestibular nuclear complex (VNC) at 2, 10, and 30 h post-surgery. Using Western blotting, the α1 and γ2 subunits (but not the β2 subunit) were detected in the VNC of labyrinthine-intact animals. However, there were no significant differences in the protein expression of the α1 and γ2 subunits within the ipsilateral or contralateral VNC at any time post-UVD compared to sham and anesthetic controls. Furthermore, UVD did not induce the expression of the β2 protein. These results suggest that vestibular compensation in guinea pig, as in the rat, is not associated with changes in the protein levels of the GABAA receptor subunits α1, β2, and γ2 in the VNC. However, a limitation of this study is that the Western blotting technique can detect only changes that are larger than 30% and therefore small changes cannot be excluded.

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Acknowledgements

This research was supported by a Project Grant from the Health Research Council of New Zealand (to P.S. and C.D.). C.G. was supported by a FRST Bright Futures PhD Scholarship. Special thanks to Dr. Ian Appleton, Dr. Ping Liu, and Dr. Yiwen Zheng for their expert advice.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, School of Medical Sciences, University of Otago, Dunedin, New Zealand

    Catherine M. Gliddon, Cynthia L. Darlington & Paul F. Smith

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  1. Catherine M. Gliddon
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  2. Cynthia L. Darlington
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  3. Paul F. Smith
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Correspondence to Paul F. Smith.

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Gliddon, C.M., Darlington, C.L. & Smith, P.F. GABAA receptor subunit expression in the guinea pig vestibular nucleus complex during the development of vestibular compensation. Exp Brain Res 166, 71–77 (2005). https://doi.org/10.1007/s00221-005-2344-4

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  • DOI: https://doi.org/10.1007/s00221-005-2344-4

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