Abstract
In the present study we used computer-controlled mechanical displacement of paired whiskers in normal and C-fiber-depleted rats to quantitatively examine the role of C-fibers in the receptive field properties of barrel cortical cells. In rodents when adjacent whiskers are stimulated prior to the main whisker responses to the main whisker are inhibited, the degree of inhibition being a function of the inter-deflection intervals. The adjacent-whisker-evoked inhibition of barrel cells in normal and C-fiber-depleted rats using neonatal capsaicin treatment were examined by stimulation of the adjacent whisker zero, 10, 20, 30, 50 and 100 ms prior to the main whisker deflection. C-fiber depletion reduced the suppressive effect of paired whisker stimulation at all of the tested inter-stimulus intervals without changing response latencies. The main effect was observed during the later phase of response (about 13–17 ms from stimulus onset) and not during the initial responses (7–12 ms). These results suggest that the inhibitory receptive field properties of low-threshold mechanical somatosensory cells are influenced by C-fibers.
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References
Armstrong-James M, Fox K (1987) Spatiotemporal convergence and divergence in the rat SI “barrel” cortex. J Comp Neurol 263:265–281
Armstrong-James M, Callahan CA, Friedman MA (1991) Thalamo-cortical processing of vibrissal information in the rat. I. Intracortical origins of surround but not center-receptive fields of layer IV neurons in the rat SI barrel field cortex. J Comp Neurol 303:193–210
Armstrong-James M, Fox K, Das GA (1992) Flow of excitation within rat barrel cortex on striking a single vibrissa. J Neurophysiol 68:1345–1358
Brumberg JC, Pinto DJ, Simons DJ (1996) Spatial gradients and inhibitory summation in the rat whisker barrel system. J Neurophysiol 76:130–140
Calford MB, Tweedale R (1991) C-fibers provide a source of masking inhibition to primary somatosensory cortex. Proc R Soc Lond B Biol Sci 243:269–275
Fitzgerald M (1983) Capsaicin and sensory neurons—a review. Pain 15:109–130
Fox K (1994) The cortical component of experience-dependent synaptic plasticity in the rat barrel cortex. J Neurosci 14:7665–7679
Goldreich D, Kyriazi HT, Simons DJ (1999) Functional independence of layer IV barrels in rodent somatosensory cortex. J Neurophysiol 82:1311–1316
Greek KA, Chowdhury SA, Rasmusson DD (2003) Interaction between inputs from adjacent digits in somatosensory thalamus and cortex of the raccoon. Exp Brain Res 151:364-371
Hiura A (2000) Neuroanatomical effects of capsaicin on the rat primary afferent neurons. Arch Histol Cytol 63:199–215
Holzer P (1991) Capsaicin: cellular targets, mechanisms of action and selectivity for thin sensory neurons. Pharmacol Rev 43:143–201
Katz DB, Simon SA, Moody A, Nicolelis MAL (1999) Simultaneous reorganization in thalamocortical ensembles evolves over several hours after perioral capsaicin injections. J Neurophysiol 82:963–977
Krahl SE, Senanayake SS, Handforth A (2001) Destruction of peripheral C-fibers does not alter subsequent vagus nerve stimulation-induced seizure suppression in rats. Epilepsia 42:586–589
Kwan CL, Hu JW, Sessle BJ (1996) Neuroplastic effects of neonatal capsaicin on neurons in adult rat trigeminal nucleus principalis and subnucleus oralis. J Neurophysiol 75:298–310
Kwan CL, Demaro JA, Hu JW, Jacquin MF, Sessle BJ (1999) C-fiber depletion alters response properties of neurons in trigeminal nucleus principalis. J Neurophysiol 81: 435–446
Kyriazi HT, Carvell GE, Brumberg JC, Simons DJ (1996) Effects of baclofen and phaclofen on receptive field properties of rat whisker barrel neurons. Brain Res 712(2):325–8
McMahon SB, Wall PD (1983) Plasticity in the nucleus gracilis of the rat. Exp Neurol 80:195–207
Mirabella G, Battiston S, Diamond ME (2001) Integration of multiple-whisker input in rat somatosensory cortex. Cereb Cortex 11:164–170
Moore CL, Nelson SB (1998) Spatio-temporal subthreshold receptive fields in the vibrissa representation of rat primary somatosensory cortex. J Neurophysiol 80:2882–2892
Nussbaumer JC, Wall PD (1985) Expansion of receptive fields in the mouse cortical barrelfield after administration of capsaicin to neonates or local application on the infraorbital nerve in adults. Brain Res 360:1–9
Shimegi S, Ichikawa T, Akasaki T, Sato H (1999) Temporal characteristics of response integration evoked by multiple whisker stimulation in the barrel cortex of rats. J Neurosci 19:10164–10175
Simons DJ (1985) Temporal and spatial integration in the rat SI vibrissa cortex. J Neurophysiol 54:615–635
Simons DJ, Carvell GE (1989) Thalamocortical response transformation in the rat vibrissa/barrel system. J Neurophysiol 61:311–330
Szallasi A (1994) The vanilloide (capsaicin) receptor: receptor types and species differences. Gen. Pharmacol 25:223–243
Wall PD, Fitzgerrald M, Nussbaumer JC, Van der Loos H, Devor M (1982) Somatotopic maps are disorganized in adult rodents treated neonatally with capsaicin. Nature 295:691–693
Welker C (1971) Microelectrode delineation of fine grain somatotopic organization of (SmI) cerebral neocortex in albino rat. Brain Res 26:259–275
Welker E, Armstrong-James M, Van der Loos H, Kraftsik R (1993) The mode of activation of a barrel column: response properties of single units in the somatosensory cortex of the mouse upon whisker deflection. Eur J Neurosci 5:691–712
Woolsey TA, Van der Loos H (1970) The structural organization of layer IV in the somatosensory region (SI) of mouse cerebral cortex: the description of a cortical field composed of discrete cytoarchitectionic unit. Brain Res 17:205–242
Zhu J, Connors BW (1999) Intrinsic firing patterns and whisker-evoked synaptic responses of neurons in the rat barrel cortex. J Neurophysiol 81:1171–1183
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This research was supported by the Neuroscience Research Center, Shaheed Beheshti University of Medical Sciences.
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Farazifard, R., Kiani, R., Noorbakhsh, M. et al. Effects of neonatal C-fiber depletion on the integration of paired-whisker inputs in rat barrel cortex. Exp Brain Res 162, 115–121 (2005). https://doi.org/10.1007/s00221-004-2118-4
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DOI: https://doi.org/10.1007/s00221-004-2118-4