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European Food Research and Technology

, Volume 213, Issue 4–5, pp 259–266 | Cite as

Tomato (Lycopersicon esculentum) allergens in pollen-allergic patients

  • Kay Foetisch
  • Dae Son
  • Friedrich Altmann
  • Holger Aulepp
  • Amedeo Conti
  • Dieter Haustein
  • Stefan Vieths
Original Paper

Abstract

This study was undertaken to investigate the relevance of tomato allergy in pollen-allergic patients, and to identify and characterize tomato allergens. Therefore, sera of a group of 32 pollen-allergic patients with a history of adverse reactions to tomato were investigated by means of immunoblotting and specific IgE measurements (CAP, EAST, EAST inhibition and CCD [cross-reactive carbohydrate determinants] – ELISA). IgE-reactive spots on 2D blots were analyzed by amino acid microsequencing.

Tomato allergy was found to occur with a prevalence of about 9% in our group of birch pollen-allergic patients. Of the patients with adverse reactions to tomato, 44% presented IgE to tomato profilin and 35.5% specific IgE to CCD. Two patients were sensitized to a lipid transfer protein in tomato. Microsequencing of other IgE-reactive proteins, separated by 2D-gel electrophoresis and transferred to PVDF membranes, revealed β-fructofuranosidase, polygalacturonase 2A, and pectinesterase as further tomato allergens. Our data show that tomato is a relevant allergenic food in pollen-allergic patients. It seems that the majority of pollen-associated allergies to tomato is caused by known ubiquitous allergenic structures such as profilin and cross-reactive IgE-binding N-glycans. Furthermore, lipid transfer protein, as well as polygalacturonase 2A, pectinesterase and β-fructofuranosidase may represent birch pollen-independent tomato allergens for certain patients.

Tomato Food allergy Profilin Carbohydrate epitopes IgE 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Kay Foetisch
    • 1
  • Dae Son
    • 2
  • Friedrich Altmann
    • 3
  • Holger Aulepp
    • 4
  • Amedeo Conti
    • 5
  • Dieter Haustein
    • 1
  • Stefan Vieths
    • 1
  1. 1.Department of Allergology, Paul-Ehrlich-Institut, Langen, GermanyGermany
  2. 2.Sungkyunkwan University School of Medicine, Seoul, KoreaKorea
  3. 3.University of Agriculture, Vienna, AustriaAustria
  4. 4.Borkum Riff Hospital, Borkum, GermanyGermany
  5. 5.CNR c/o Bioindustry Park, Colleretto Giacosa, ItalyItaly

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