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European Food Research and Technology

, Volume 213, Issue 2, pp 107–112 | Cite as

Characterisation of the Roundup Ready soybean insert

  • Pieter Windels
  • Isabel Taverniers
  • Ann Depicker
  • Erik Van Bockstaele
  • Marc De Loose
Original paper

Abstract.

In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3′ NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products.

Roundup Ready soybean Junction fragment Rearrangements GMO Identification 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Pieter Windels
    • 1
  • Isabel Taverniers
    • 1
  • Ann Depicker
    • 2
  • Erik Van Bockstaele
    • 1
  • Marc De Loose
    • 1
  1. 1.Department for Plant Genetics and Breeding, Centre for Agricultural Research, Caritasstraat 21, 9090 Melle, Belgium
  2. 2.Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium

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