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Study on the antibacterial activity and mechanism of Cinnamaldehyde against Methicillin-resistant Staphylococcus aureus

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Abstract

The antibacterial activity of Cinnamaldehyde against Methicillin-resistant Staphylococcus aureus in Bovine Mastitis is investigated in this study, providing insights into inhibition mechanisms and elucidating the effects on bacterial cell membranes. The Minimum Inhibitory Concentration and Minimum Bactericidal Concentration were determined in this study. In addition, growth curves and a time-kill assay were constructed to assess the antibacterial activity of Cinnamaldehyde. The study revealed that the MIC values ranged from 62.5 to 125 μg/mL, and the MBC values ranged from 125 to 250 μg/mL. The presence of sublethal concentrations of Cinnamaldehyde impeded bacterial growth, while high concentrations demonstrated a significant and rapid bactericidal effect. Subsequently, we examined cell morphology using SEM and TEM, evaluated membrane integrity via laser confocal fluorescence microscopy, and measured levels of β-galactosidase, extracellular DNA release, LDH activity, and ROS, to assess the antibacterial mechanism of Cinnamaldehyde. The findings indicated that with higher concentrations of Cinnamaldehyde, Methicillin-resistant Staphylococcus aureus demonstrated significant morphological alterations and disruption of both the cell wall and membrane. Furthermore, Cinnamaldehyde disrupted the integrity of membranes and increased permeability of the outer membrane in a manner dependent on its concentration. Cinnamaldehyde notably triggered the release of β-galactosidase, extracellular DNA, and LDH, in addition to elevating cellular ROS levels. Finally, the effect of Cinnamaldehyde on the transcription levels of genes related to cell membrane synthesis was assessed using RT-qPCR, and the effect of Cinnamaldehyde on the total protein content of Methicillin-resistant Staphylococcus aureus cells was assessed using WB. The RT-qPCR results showed that Cinnamaldehyde at 1xMIC notably upregulated the transcription levels of genes related to fatty acid biosynthesis in Methicillin-resistant Staphylococcus aureus cell membranes, with a significant or highly significant effect. The WB results showed that Cinnamaldehyde exerts its antibacterial action by suppressing protein expression in Methicillin-resistant Staphylococcus aureus. These findings illustrate that Cinnamaldehyde exerts a potent inhibitory effect on Methicillin-resistant Staphylococcus aureus, establishing a fundamental foundation for the potential use of Cinnamaldehyde essential oil as an antibacterial agent in the treatment of bovine mastitis, in accordance with established scientific standards.

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The data underlying this article will be shared on reasonable request to the corresponding author.

References

  1. Das A, Guha C, Biswas U et al (2017) Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal. Vet World 10(5):517–520

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Vasudevan P, Nair MK, Annamalai T et al (2003) Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet Microbiol 92(1–2):179–185

    CAS  PubMed  Google Scholar 

  3. Peacock SJ, Paterson GK (2015) Mechanisms of methicillin resistance in Staphylococcus aureus. Annu Rev Biochem 84:577–601

    CAS  PubMed  Google Scholar 

  4. Yoneda A, Thanert R, Burnham CD et al (2020) In vitro activity of meropenem/piperacillin/tazobactam triple combination therapy against clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus pseudintermedius and vancomycin-resistant Enterococcus spp. Int J Antimicrob Agents 55(2):105864

    CAS  PubMed  Google Scholar 

  5. Mooyottu S, Kollanoor-Johny A, Flock G et al (2014) Carvacrol and trans-cinnamaldehyde reduce Clostridium difficile toxin production and cytotoxicity in vitro. Int J Mol Sci 15(3):4415–4430

    PubMed  PubMed Central  Google Scholar 

  6. Li XY, Erhunmwunsee F, Liu M et al (2022) Antimicrobial mechanisms of spice essential oils and application in food industry. Food Chem 382:132312

    CAS  PubMed  Google Scholar 

  7. Dogruyol H, Mol S, Cosansu S (2020) Increased thermal sensitivity of Listeria monocytogenes in sous-vide salmon by oregano essential oil and citric acid. Food Microbiol 90:103496

    CAS  PubMed  Google Scholar 

  8. Polat YG, Delaquis P (2020) Natural compounds with antibacterial activity against Cronobacter spp. in powdered infant formula: a review. Front Nutr 7:595964

    Google Scholar 

  9. Albano M, Crulhas BP, Alves F et al (2019) Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis. Microb Pathog 126:231–238

    CAS  PubMed  Google Scholar 

  10. Lizi Y, Jiehao C, Kaiyu W et al (2020) Study the antibacterial mechanism of cinnamaldehyde against drug-resistant Aeromonas hydrophila in vitro. Microb Pathog 145:1 (Prepublish)

    Google Scholar 

  11. Shen Q, Zhou W, Hu L et al (2017) Bactericidal activity of alpha-bromocinnamaldehyde against persisters in Escherichia coli. PLoS ONE 12(7):e182122

    Google Scholar 

  12. Kim YG, Lee JH, Kim SI et al (2015) Cinnamon bark oil and its components inhibit biofilm formation and toxin production. Int J Food Microbiol 195:30–39

    CAS  PubMed  Google Scholar 

  13. Kot B, Wicha J, Piechota M et al (2015) Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli. Turk J Med Sci 45(4):919–924

    CAS  PubMed  Google Scholar 

  14. Kot B, Wierzchowska K, Gruzewska A et al (2018) The effects of selected phytochemicals on biofilm formed by five methicillin-resistant Staphylococcus aureus. Nat Prod Res 32(11):1299–1302

    CAS  PubMed  Google Scholar 

  15. Wang P, Ma L, Jin J et al (2019) The anti-aflatoxigenic mechanism of cinnamaldehyde in Aspergillus flavus. Sci Rep 9(1):10499

    ADS  PubMed  PubMed Central  Google Scholar 

  16. He Z, Huang Z, Jiang W et al (2019) Antimicrobial activity of cinnamaldehyde on Streptococcus mutans biofilms. Front Microbiol 10:2241

    PubMed  PubMed Central  Google Scholar 

  17. Jia P, Xue YJ, Duan XJ et al (2011) Effect of cinnamaldehyde on biofilm formation and sarA expression by methicillin-resistant Staphylococcus aureus. Lett Appl Microbiol 53(4):409–416

    CAS  PubMed  Google Scholar 

  18. Kim Y, Kim S, Cho KH et al (2022) Antibiofilm activities of cinnamaldehyde analogs against uropathogenic Escherichia coli and Staphylococcus aureus. Int J Mol Sci 23(13):1

    CAS  Google Scholar 

  19. Doyle AA, Stephens JC (2019) A review of cinnamaldehyde and its derivatives as antibacterial agents. Fitoterapia 139:104405

    CAS  PubMed  Google Scholar 

  20. Wu SC, Yang ZQ, Liu F et al (2019) Antibacterial effect and mode of action of flavonoids from licorice against methicillin-resistant Staphylococcus aureus. Front Microbiol 10:2489

    ADS  PubMed  PubMed Central  Google Scholar 

  21. Janekrongtham C, Dejburum P, Sujinpram S et al (2022) Outbreak of seafood-related food poisoning from undetectable Vibrio parahaemolyticus-like pathogen, Chiang Mai Province, Thailand, December 2020. Trop Med Int Health 27(1):92–98

    CAS  PubMed  Google Scholar 

  22. Campion A, Morrissey R, Field D et al (2017) Use of enhanced nisin derivatives in combination with food-grade oils or citric acid to control Cronobacter sakazakii and Escherichia coli O157:H7. Food Microbiol 65:254–263

    CAS  PubMed  Google Scholar 

  23. Raquel R, María V, Amparo C (2019) Study of the potential synergistic antibacterial activity of essential oil components using the thiazolyl blue tetrazolium bromide (MTT) assay. LWT 101:1

    Google Scholar 

  24. Ahmad A, Viljoen A (2015) The in vitro antimicrobial activity of Cymbopogon essential oil (lemon grass) and its interaction with silver ions. Phytomedicine 22(6):657–665

    CAS  PubMed  Google Scholar 

  25. Brochot A, Guilbot A, Haddioui L et al (2017) Antibacterial, antifungal, and antiviral effects of three essential oil blends. Microbiologyopen 6(4):1

    Google Scholar 

  26. Bedoya-Serna CM, Dacanal GC, Fernandes AM et al (2018) Antifungal activity of nanoemulsions encapsulating oregano (Origanum vulgare) essential oil: in vitro study and application in Minas Padrao cheese. Braz J Microbiol 49(4):929–935

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Wang S, Kang OH, Kwon DY (2021) Trans-cinnamaldehyde exhibits synergy with conventional antibiotic against methicillin-resistant Staphylococcus aureus. Int J Mol Sci 22(5):1

    ADS  Google Scholar 

  28. Dhara L, Tripathi A (2020) Cinnamaldehyde: a compound with antimicrobial and synergistic activity against ESBL-producing quinolone-resistant pathogenic Enterobacteriaceae. Eur J Clin Microbiol Infect Dis 39(1):65–73

    PubMed  Google Scholar 

  29. Chan LL, Mcculley KJ, Kessel SL (2017) Assessment of cell viability with single-, dual-, and multi-staining methods using image cytometry. Methods Mol Biol 1601:27–41

    CAS  PubMed  Google Scholar 

  30. Ma MM (2022) Antibacterial activity and mechanism of monodecanoate against Escherichia coli and Staphylococcus aureus. Nanchang University, p 196

  31. Yunbin Z, Xiaoyu L, Yifei W et al (2016) Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control 59:1

    Google Scholar 

  32. Wang LH, Wang MS, Zeng XA et al (2017) An in vitro investigation of the inhibitory mechanism of β-galactosidase by cinnamaldehyde alone and in combination with carvacrol and thymol. Biochim Biophys Acta Gen Subj 1861(1):3189–3198

    CAS  PubMed  Google Scholar 

  33. He XW, Dai YY, Li XY et al (2020) Antibacterial mechanism of cinnamaldehyde on salmonella typhimuriumin vitro. Acta Agric Univ Jiangxiensis 42(01):150–156

    Google Scholar 

  34. Ce S, Xiaowei Z, Xingchen Z et al (2017) Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk. Food Control 71:1

    Google Scholar 

  35. Sheikh S, Waseem AW, Jawad MB et al (2016) Cinnamaldehyde and its derivatives, a novel class of antifungal agents. Fitoterapia 112:1

    Google Scholar 

  36. Richardson AR, Libby SJ, Fang FC (2008) A nitric oxide-inducible lactate dehydrogenase enables Staphylococcus aureus to resist innate immunity. Science 319(5870):1672–1676

    ADS  CAS  PubMed  Google Scholar 

  37. Qi M, Zhao R, Liu Q, Yan H, Zhang Y, Wang S, Yuan Y (2021) Antibacterial activity and mechanism of high voltage electrostatic field (HVEF) against Staphylococcus aureus in medium plates and food systems. Food Control 120:107566

    CAS  Google Scholar 

  38. Zhan X, Tan Y, Cheng X et al (2022) Effects of cinnamaldehyde against planktonic bacteria and biofilm formation of Shigella flexneri. Microb Pathog 171:1

    Google Scholar 

  39. Goel S, Mishra P (2018) Thymoquinone inhibits biofilm formation and has selective antibacterial activity due to ROS generation. Appl Microbiol Biotechnol 102(4):1955–1967

    CAS  PubMed  Google Scholar 

  40. Turk M, Mejanelle L, Sentjurc M et al (2004) Salt-induced changes in lipid composition and membrane fluidity of halophilic yeast-like melanized fungi. Extremophiles 8(1):53–61

    CAS  PubMed  Google Scholar 

  41. Pang D, Liao S, Wang W et al (2019) Destruction of the cell membrane and inhibition of cell phosphatidic acid biosynthesis in Staphylococcus aureus: an explanation for the antibacterial mechanism of morusin. Food Funct 10(10):6438–6446

    CAS  PubMed  Google Scholar 

  42. Wang LH, Zeng XA, Wang MS et al (2018) Modification of membrane properties and fatty acids biosynthesis-related genes in Escherichia coli and Staphylococcus aureus: Implications for the antibacterial mechanism of naringenin. Biochim Biophys Acta Biomembr 1860(2):481–490

    CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (32160852). Thanks to all the teachers and students who have contributed to this paper.

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Authors and Affiliations

  1. College of Animal Science and Technology, Ningxia University, Yinchuan, China

    Xiaohui Chen, Panpan Liu, Xiaofeng Luo, Ailin Huang & Guiqin Wang

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  1. Xiaohui Chen
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  2. Panpan Liu
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  3. Xiaofeng Luo
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  4. Ailin Huang
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  5. Guiqin Wang
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Contributions

XC: Conceptualization, methodology, writing—original draft. PL: Visualization, investigation. XL: Visualization, investigation. AH: Supervision. GW: Writing—review and editing.

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Correspondence to Guiqin Wang.

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Chen, X., Liu, P., Luo, X. et al. Study on the antibacterial activity and mechanism of Cinnamaldehyde against Methicillin-resistant Staphylococcus aureus. Eur Food Res Technol 250, 1069–1081 (2024). https://doi.org/10.1007/s00217-023-04446-z

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