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Impact of barley endophytic Pantoea agglomerans on the malt filterability

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Abstract

The microbial metabolic activity during malting is an important factor affecting malt quality besides barley varieties. Elucidating the effect and action mechanism of dominant microbial on malt quality will provide a new strategy for the precise control of malt quality. Pantoea spp. was identified as the dominant bacteria of barley by the aid of high-throughput sequencing technique. Moreover, the dilution plating results showed that P. agglomerans was the dominant bacteria in quantity. During malting, the number of P. agglomerans did not change significantly after steeping, but increased exponentially during germination. The quality of malt, especially the filtration rate, is affected by the inoculation of P. agglomerans in the malting process. The exopolysaccharides of P. agglomerans was the main factor leading to the decrease of wort filtration speed. When the exopolysaccharides of P. agglomerans in the wort reached at 12.5 μg/mL, the filtration speed decreased by 50%.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31871785), and the Program of Introducing Talents of Discipline to Universities (111 Project) (111-2-06)

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, People’s Republic of China

    Bingxin Han & Guolin Cai

  2. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Bingxin Han, Ying Xie, Jian Lu & Guolin Cai

  3. Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, People’s Republic of China

    Bingxin Han & Guolin Cai

  4. Jiangsu Nongken Malt Company Limited, Yancheng, 224399, People’s Republic of China

    Ming Zhang

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  1. Bingxin Han
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  4. Jian Lu
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  5. Guolin Cai
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Contributions

Conceptualization: BH and GC; methodology: BH, YX, MZ, and GC; formal analysis and investigation: BH and GC; writing—original draft preparation: BH; writing—review and editing: BH and GC; funding acquisition: GC; supervision: GC and JL.

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Correspondence to Guolin Cai.

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The authors declare that they have not known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Han, B., Xie, Y., Zhang, M. et al. Impact of barley endophytic Pantoea agglomerans on the malt filterability. Eur Food Res Technol 249, 1403–1409 (2023). https://doi.org/10.1007/s00217-023-04223-y

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  • DOI: https://doi.org/10.1007/s00217-023-04223-y

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