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Quantitative proteome analysis revealed metabolic changes in Arthrospira platensis in response to selenium stress

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Abstract

Due to its high nutritional value and selenium tolerance, selenium-enriched Arthrospira platensis was a promising source for dietary Se supplementation. However, the regulatory mechanism involved in selenium enrichment in A. platensis has not been completely elucidated. In this study, we investigated the changes in physiological and proteome profiles of selenium-enriched A. platensis (Se-AP). At 30 mg/mL Na2SeO3, organic selenium concentration of A. platensis was 52.94 mg/kg. Se-AP proteins exhibited stronger antioxidant activity than the native selenium-free A. platensis (AP) proteins according to total reducing power, 2,2ʹ-azinobis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity. Using a tandem mass tag-based (TMT) quantitative proteomics method, 948 differentially expressed proteins were identified when A. platensis was cultured with 30 mg/L Na2SeO3. Following GO functional annotation and KEGG enrichment analysis, we found that the photosynthetic and ribosome pathways were significantly inhibited, while oxidative phosphorylation, the tricarboxylic acid cycle, prokaryote carbon fixation, amino acid synthesis, ATP-binding cassette (ABC) transporters, and antioxidant activity were markedly enhanced, improving the adaptation of A. platensis under selenium stress. Our findings will contribute to further understanding the physiological response of A. platensis against selenium stress, providing a potential strategy for developing selenium-enriched A. platensis.

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Acknowledgements

This study was financially supported by the Key Projects in Guangxi (2019GXNSFDA245008), Guangxi Natural Science Foundation (2017GXNSFAA198297), Guangxi Science and Technology Major Special Project (AA17204075;AA17202010-3), and Guangxi Natural Science Foundation (2020GXNSFAA297038)

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Authors and Affiliations

  1. College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China

    Miao Wang, Jinhao Meng, Li Huang, Yunxia Bai, Xiaoling Liu & Shubo Li

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  1. Miao Wang
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  2. Jinhao Meng
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  3. Li Huang
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  4. Yunxia Bai
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  5. Xiaoling Liu
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  6. Shubo Li
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Contributions

MW: experiments, data curation, and writing; JM: validation and investigation; LH: experiment assistant; YB: methodology; XL: resources and funding acquisition; SL: supervision, conceptualization, and funding acquisition.

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Correspondence to Shubo Li.

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We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with this manuscript. We have no financial or personal relationships with other individuals or organizations that can inappropriately influence our work.

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The authors Miao Wang, Jinhao Meng, Li Huang, Yunxia Bai, Xiaoling Liu and Shubo Li hereby confirm that this manuscript is performed according to and follows the COPE guidelines and has not already been published nor is it under consideration for publication elsewhere. This article does not contain any studies with human or animal subjects.

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Wang, M., Meng, J., Huang, L. et al. Quantitative proteome analysis revealed metabolic changes in Arthrospira platensis in response to selenium stress. Eur Food Res Technol 248, 839–856 (2022). https://doi.org/10.1007/s00217-021-03917-5

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