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Development of a sensitive sandwich enzyme-linked immunosorbent assay test kit for reliable detection of peanut residues in processed food

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Abstract

“Hidden” peanut allergens in commercial foods pose a potential risk for peanut-allergic individuals. Sensitive and reliable analytical methods are urgently needed for detecting peanut in processed foods at low levels. We developed a peanut sandwich ELISA test kit by pairing two polyclonal sera against peanut proteins obtained from different spices. Its analytical performance of sensitivity, specificity, accuracy, trueness, and precision were evaluated, respectively. The limit of detection (LOD) was defined as 0.001 mg/kg, and no cross-reactivity was observed in 25 spices including legumes, tree nuts, and seeds. Besides, the mean recoveries in four spiked food matrixes ranged from 74.067 to 122.953%, and the recoveries of the three model foods incurred with peanut proteins were within the range of 80–120%. Acceptable results of repeatability and reproducibility were obtained referring to the AOAC standard. Moreover, it was verified to be capable of applying for detecting peanut residues in commercially available food products in the market and evaluating the food labels effectively. The study provides powerful technical support for the sensitive detection of peanut products for both food manufacturers and regulatory agencies.

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (No. 32072338).

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, 266003, Shandong Province, People’s Republic of China

    Yu Ji, Hong Lin, Jinlong Zhao & Zhenxing Li

  2. Agro-Product Safety Research Center Chinese Academy of Inspection and Quarantine, CAIQ 11 Ronghua Nanlu, Yi Zhuang, Beijing, 100176, People’s Republic of China

    Jiukai Zhang

  3. Qingdao Pribolab Biotech Co., Ltd, Qingdao, 266101, Shandong Province, People’s Republic of China

    Hongbing Liu

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  1. Yu Ji
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  2. Hong Lin
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  3. Jinlong Zhao
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  4. Jiukai Zhang
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Correspondence to Zhenxing Li.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Ji, Y., Lin, H., Zhao, J. et al. Development of a sensitive sandwich enzyme-linked immunosorbent assay test kit for reliable detection of peanut residues in processed food. Eur Food Res Technol 248, 273–282 (2022). https://doi.org/10.1007/s00217-021-03879-8

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  • DOI: https://doi.org/10.1007/s00217-021-03879-8

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