Skip to main content
SpringerLink
Log in

High-performance liquid chromatography for the sensitive zearalenone determination by the automated immunomagnetic beads purifier for one-step sample pre-treatment

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

In this study, a sensitive high-performance liquid chromatography method for zearalenone determination in feeds and grains was established based on the automatic immunomagnetic bead purifier analyzer (KY-AMI-I) for the one-step sample pre-treatment. Various parameters that may affect the analytical performance were investigated. Immunomagnetic bead separation system based on a monoclonal antibody (anti-ZEN antibody-immunomagnetic beads conjugation) was individually prepared for the ZEN purification. Besides, the average recoveries in spiked samples ranged from 75.23 to 102.62%, and the relative standard deviations were between 3.55 and 8.07% (< 10%). The method was used to analyze naturally contaminated positive feedstuff, and the results obtained by this method was in a good agreement with data obtained by immunoaffinity column-high-performance liquid chromatography (IAC-HPLC–FLD). The conformity and correlation of two methods was satisfactory. The comprehensive results showed that the proposed method was shown to be a suitable method for ZEN determination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Pan MF, Ma TY, Yang JY, Li SJ (2020) Development of lateral flow immunochromatographic assays using colloidal Au sphere and nanorods as signal marker for the determination of zearalenone in cereals. Foods 9:281–291

    Article  CAS  Google Scholar 

  2. Ozgur G, Bulent K (2020) Occurrence of deoxynivalenol and zearalenone in cereals and cereal products from Turkey. Food Control 110:106982

    Article  CAS  Google Scholar 

  3. Sven D, Janine W (2015) Invited review: diagnosis of zearalenone (ZEN) exposure of farm animals and transfer of its residues into edible tissues (carry over). Food Chem Toxicol 84:225–249

    Article  CAS  Google Scholar 

  4. Zhao Y, Yuan YC, Bai XL, Liu YM, Wu GF, Yang FS, Liao X (2020) Multi-mycotoxins analysis in liquid milk by UHPLC-Q-Exactive HRMS after magnetic solid-phase extraction based on PEGylated multi-walled carbon nanotubes. Food Chem 305:125429

    Article  CAS  Google Scholar 

  5. Masayoshi T, Keiko M, Jun W, Junko I (2014) Minimization of carryover for high-throughput liquid chromatography with tandem mass spectrometry analysis of 14 mycotoxins in corn grits. J Sep Sci 37:1552–1560

    Article  CAS  Google Scholar 

  6. Zhang Y, Liu DH, Peng J, Cui YR, Shi Y, He H (2019) Magnetic hyperbranched molecularly imprinted polymers for selective enrichment and determination of zearalenone in wheat proceeded by HPLC-DAD analysis. Talanta 209:120555

    Article  CAS  Google Scholar 

  7. Zhao FC, Shen Q, Wang HM, Han X, Yang ZY (2017) Development of a rapid magnetic bead-based immunoassay for sensitive detection of zearalenone. Food Control 79:227–233

    Article  CAS  Google Scholar 

  8. He BS, Yan XH (2020) Ultrasensitive electrochemical aptasensor based on CoSe2/AuNRs and 3D structured DNA-PtNi@Co-MOF networks for the detection of zearalenone. Sens Actuators B Chem 306:127558

    Article  CAS  Google Scholar 

  9. Katsuki T, Emiko K, Seiichi U, Ayaka S (2017) Gas chromatography-mass spectrometry for metabolite profiling of Japanese black cattle naturally contaminated with zearalenone and sterigmatocystin. Toxins 9:294

    Article  CAS  Google Scholar 

  10. Ma ZH, Huang B, Zhang J, Zhang Y (2009) Time-resolved fluoroimmunoassay of zearalenone in cereals with a europium chelate as label. J Rare Earths 27(6):1088–1091

    Article  Google Scholar 

  11. Wang YG, Zhao GH, Wang H, Zhang Y, Zhang N, Wei D (2020) Label-free electrochemical immunosensor based on biocompatible nanoporous Fe3O4 and biotin-streptavidin system for sensitive detection of zearalenone. Analyst 145:1368

    Article  CAS  Google Scholar 

  12. Mine Antepa H, Melek M (2012) Development of new dispersive liquid-liquid microextraction technique for the identification of zearalenone in beer. Anal Methods 4:4129–4134

    Article  CAS  Google Scholar 

  13. Pornpimol T, Wilairat C, Kwok FC, Thitima R (2018) Selective magnetic nanographene oxide solid-phase extraction with high-performance liquid chromatography and fluorescence detection for the determination of zearalenone in corn samples. J Sep Sci 41:4348–4354

    Article  CAS  Google Scholar 

  14. Roswitha G, Klaus L (2004) Simultaneous determination of aflatoxins, ochratoxin A, and zearalenone in grains by new immunoaffinity column/liquid chromatography. J AOAC Int 87:411–416

    Article  Google Scholar 

  15. Liu RP, Wang C, Jiang Q, Zhang W (2013) Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen. Anal Chim Acta 801:91–96

    Article  CAS  Google Scholar 

  16. Urusov AE, Petrakova AV, Vozniak MV, Zherdev AV, Dzantie BB (2014) Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles. Sensors 14:21843–21857

    Article  CAS  Google Scholar 

  17. Zhang Z, Guan YP, Xia TT, Du J, Li T, Sun ZY, Guo C (2017) Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay. Colloids Surf A 520:335–342

    Article  CAS  Google Scholar 

  18. Fu XL, Liu YY, Qiu RY, Foda M, Zhang Y (2018) The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer. Biochemistry and Biophysics Reports 13:73–77

    Article  Google Scholar 

  19. Li WK, Zhang HX, Shi YP (2018) Simultaneous determination of aflatoxin B1 and zearalenone by magnetic nanoparticles filled amino-modified multi-walled carbon nanotubes. Anal Methods 10:3353–3363

    Article  CAS  Google Scholar 

  20. Zhang HS, Qi SW, Rao J, Li QL (2013) Development of a rapid and high-performance chemiluminescence immunoassay based on magnetic particles for protein S100B in human serum. Luminescence 28:927–932

    Article  CAS  Google Scholar 

  21. National Health and Family Planning Commission of the People’s Republic of China; China Food and Drug Administration. National Standard of the People’s Republic of China (GB5009.209–2016): National Food Safety Standard-Determination of Zearalenone in Cereals

Download references

Funding

The authors acknowledge the financial support from the project of the National Special Project for Agro-product Safety Risk Evaluation of China (GJFP2019002), National Natural Science Foundation of China (81860399), Provincial Youth Science Fund of Jiangxi Province (20142BAB215072), and The Science and Technology Key R&D Program of Jiangxi Province (20202BBG72001).

Author information

Authors and Affiliations

  1. School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China

    Bo Zhang, Zhenjiang Liu & Daolin Du

  2. Kangyuan Techbio Biological Technology Co., Ltd, Suqian, 223600, China

    Bo Zhang

  3. College of Pharmacy, Nankai University, Tianjin, 300350, China

    Wenting Liu

  4. The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Xiaoling Fu

Authors
  1. Bo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenting Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhenjiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoling Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Daolin Du
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiaoling Fu or Daolin Du.

Ethics declarations

Conflict of interest

Bo Zhang declares that he has no conflict of interest. Wenting Liu declares that she has no conflict of interest. Zhenjiang Liu declares that he has no conflict of interest. Xiaoling Fu declares that he has no conflict of interest. Daolin Du declares that he has no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animals.

Informed consent

Informed consent is not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, B., Liu, W., Liu, Z. et al. High-performance liquid chromatography for the sensitive zearalenone determination by the automated immunomagnetic beads purifier for one-step sample pre-treatment. Eur Food Res Technol 248, 109–117 (2022). https://doi.org/10.1007/s00217-021-03862-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-021-03862-3

Keywords

Search

Navigation