Skip to main content
SpringerLink
Log in

Application of ionic liquid-based air-assisted dispersive liquid–liquid microextraction combined with high-performance liquid chromatography for the determination of six tetracyclines in honey

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

A novel ionic liquid-based air-assisted dispersive liquid–liquid microextraction combined with high-performance liquid chromatographic method was developed for the simultaneous analysis of oxytetracycline, tetracycline, demeclocycline, metacycline, chlortetracycline and doxycycline in honey. Tetracyclines in samples were extracted with ethyl acetate-[Bmim]PF6 under acidic condition with the assistance of air. The analytes were separated on a C18 column thermostated at 20 °C under gradient condition with a mobile phase consisted of methanol and oxalic acid. Parameters for the extraction (pH, dilution buffer, ionic strength, volumes of ionic liquid and ethyl acetate, assistance techniques and extraction time) were evaluated. The developed method showed good linearities over the range of 10.0–500 μg/L (R2 > 0.999). The enrichment factors were 16.6–41.2. The detection limits and quantification limits were 0.434–0.919 μg/kg and 1.45–3.06 μg/kg, respectively. The recoveries were 89.5–116%. The intra-day and inter-day RSDs were 0.644–6.44% and 2.35–15.9%, respectively. The developed method is sensitive, accurate, rapid, convenient, and is applicable for the determination of six tetracyclines in honey.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Jiang T, Peng Z, Xie M, Fang X, Hong Y, Huang Z, Gao W, Zhou Z, Li L, Zhu Z (2020) Rapid analysis of tetracycline in honey by microwave plasma torch mass spectrometry with ablation samples. Anal Methods 12:535–543. https://doi.org/10.1039/c9ay01887e

    Article  CAS  Google Scholar 

  2. Zhang Y, Li X, Li H, Zhang Q, Gao Y, Li X (2019) Antibiotic residues in honey: a review on analytical methods by liquid chromatography tandem mass spectrometry. TrAC-Trend Anal Chem 110:344–356. https://doi.org/10.1016/j.trac.2018.11.015

    Article  CAS  Google Scholar 

  3. The Japan Food Chemical Research Foundation (2015) Table of MRLs for food types-honey (including royal-jelly). http://db.ffcr.or.jp/front/food_group_detail?id=20600. Accessed 4 Jan 2021

  4. Ministry of Health of the People’s Republic of China, Standardization Administration of China (2003) GB 14963-2003. National food safety standard-Hygienic standard for honey

  5. Wang G, Zhou T, Lei Y (2020) Exploration of a novel triazine-based covalent organic framework for solid-phase extraction of antibiotics. Rsc Adv 10:11557–11564. https://doi.org/10.1039/c9ra10846g

    Article  CAS  Google Scholar 

  6. Di X, Zhao X, Guo X (2020) Hydrophobic deep eutectic solvent as a green extractant for high-performance liquid chromatographic determination of tetracyclines in water samples. J Sep Sci 43:3129–3135. https://doi.org/10.1002/jssc.202000477

    Article  CAS  PubMed  Google Scholar 

  7. Gao J, Wang H, Qu J, Wang H, Wang X (2017) Development and optimization of a naphthoic acid-based ionic liquid as a “non-organic solvent microextraction” for the determination of tetracycline antibiotics in milk and chicken eggs. Food Chem 215:138–148. https://doi.org/10.1016/j.foodchem.2016.07.138

    Article  CAS  PubMed  Google Scholar 

  8. Sereshti H, Semnani Jazani S, Nouri N, Shams G (2020) Dispersive liquid–liquid microextraction based on hydrophobic deep eutectic solvents: application for tetracyclines monitoring in milk. Microchem J 158:105269. https://doi.org/10.1016/j.microc.2020.105269

    Article  CAS  Google Scholar 

  9. Kaynaker M, Antep M, Merdivan M (2018) Determination of tetracyclines in milk, eggs and honey using in-situ ionic liquid based dispersive liquid-liquid microextraction. J Anal Chem 73:23–29. https://doi.org/10.1134/s1061934818010070

    Article  CAS  Google Scholar 

  10. Yang X, Zhang S, Yu W, Liu Z, Lei L, Li N, Zhang H, Yu Y (2014) Ionic liquid-anionic surfactant based aqueous two-phase extraction for determination of antibiotics in honey by high-performance liquid chromatography. Talanta 124:1–6. https://doi.org/10.1016/j.talanta.2014.02.039

    Article  CAS  PubMed  Google Scholar 

  11. Han J, Wang Y, Yu C, Yan Y, Xie X (2011) Extraction and determination of chloramphenicol in feed water, milk, and honey samples using an ionic liquid/sodium citrate aqueous two-phase system coupled with high-performance liquid chromatography. Anal Bioanal Chem 399(3):1295–1304. https://doi.org/10.1007/s00216-010-4376-2

    Article  CAS  PubMed  Google Scholar 

  12. Xu X, Su R, Zhao X, Zhuang L, Zhang Y, Li D, Li X, Zhang H, Wang Z (2011) Ionic liquid-based microwave-assisted dispersive liquid–liquid microextraction and derivatization of sulfonamides in river water, honey, milk, and animal plasma. Anal Chim Acta 707(1–2):92–99. https://doi.org/10.1016/j.aca.2011.09.018

    Article  CAS  PubMed  Google Scholar 

  13. Yang Y, Yin S, Wu L, Li Y, Sun C (2021) Determination of six tetracyclines in eggs and chicken by dispersive liquid-liquid microextraction combined with high-performance liquid chromatography. J AOAC Int. https://doi.org/10.1093/jaoacint/qsab082

    Article  PubMed  Google Scholar 

  14. Jha RR, Singh C, Pant AB, Patel DK (2018) Ionic liquid based ultrasound assisted dispersive liquid-liquid micro-extraction for simultaneous determination of 15 neurotransmitters in rat brain, plasma and cell samples. Anal Chim Acta 1005:43–53. https://doi.org/10.1016/j.aca.2017.12.015

    Article  CAS  PubMed  Google Scholar 

  15. Yin S, Yang Y, Zhang J, Li Y, Wu L, Sun C (2020) A novel ionic liquid-based liquid-liquid microextraction combined with high performance liquid chromatography for simultaneous determination of eight vitamin E isomers in human serum. J AOAC Int 103:989–996. https://doi.org/10.1093/jaoacint/qsz039

    Article  PubMed  Google Scholar 

  16. Xu X, Liu Z, Zhao X, Su R, Zhang Y, Shi J, Zhao Y, Wu L, Ma Q, Zhou X, Zhang H, Wang Z (2013) Ionic liquid-based microwave-assisted surfactant-improved dispersive liquid-liquid microextraction and derivatization of aminoglycosides in milk samples. J Sep Sci 36:585–592. https://doi.org/10.1002/jssc.201200801

    Article  CAS  PubMed  Google Scholar 

  17. Hou D, Guan Y, Di X (2011) Temperature-induced ionic liquids dispersive liquid-liquid microextraction of tetracycline antibiotics in environmental water samples assisted by complexation. Chromatographia 73:1057–1064. https://doi.org/10.1007/s10337-011-1992-8

    Article  CAS  Google Scholar 

  18. Santana A, Santana M, Pereira P (2018) Development of a method based on DLLME and UFLC-DAD for the determination of antibiotics in honey samples and the study of their degradation kinetics. J Brazil Chem Soc 29:1538–1550. https://doi.org/10.21577/0103-5053.20180028

    Article  CAS  Google Scholar 

  19. Zhang Y, Sun Y, Yang Z, Jin S, Gao L, He L, Jiang X (2020) A simple one-step transferred sample preparation for effective purification and extraction of auramine O in bean product by combining air-assisted ionic liquid-based dispersive liquid-liquid microextraction. Microchem J 159:105571. https://doi.org/10.1016/j.microc.2020.105571

    Article  CAS  Google Scholar 

  20. Nedaei M, Abdi K, Ghorbanian SA, Pirooznia N (2020) Ultrasonic-air-assisted solidification of settled organic drop microextraction using terpene-based deep eutectic solvents for the effectual enrichment of methadone in biological samples. Chromatographia 83:1413–1421. https://doi.org/10.1007/s10337-020-03952-6

    Article  CAS  Google Scholar 

  21. Farajzadeh MA, Mohebbi A, Pazhohan A, Nemati M, Mogaddam MRA (2020) Air-assisted liquid-liquid microextraction; principles and applications with analytical instruments. TrAC-Trend Anal Chem 122:115734. https://doi.org/10.1016/j.trac.2019.115734

    Article  CAS  Google Scholar 

  22. Zhang K, Li S, Wang Y, Fan J, Zhu G (2020) Air-assisted liquid-liquid microextraction based on solidification of floating deep eutectic solvent for the analysis of ultraviolet filters in water samples by high performance liquid chromatography with the aid of response surface methodology. J Chromatogr A 1618:460876. https://doi.org/10.1016/j.chroma.2020.460876

    Article  CAS  PubMed  Google Scholar 

  23. Lv Y, Bai H, He Y, Yang J, Ouyang Z, Ma Q (2020) Accelerated air-assisted in-syringe extraction and needle spray ionization coupled with miniature mass spectrometry: a streamlined platform for rapid on-site analysis. Anal Chim Acta 1136:106–114. https://doi.org/10.1016/j.aca.2020.08.043

    Article  CAS  PubMed  Google Scholar 

  24. Khoshmaram L, Saadati M, Karimi A (2020) A simple and rapid technique for the determination of copper based on air-assisted liquid-liquid microextraction and image colorimetric analysis. Anal Methods 12:3490–3498. https://doi.org/10.1039/d0ay00706d

    Article  CAS  PubMed  Google Scholar 

  25. Wang L, Huang T, Cao HX, Yuan QX, Liang ZP, Liang GX (2016) Application of air-assisted liquid-liquid microextraction for determination of some fluoroquinolones in milk powder and egg samples: comparison with conventional dispersive liquid-liquid microextraction. Food Anal Method 9:2223–2230. https://doi.org/10.1007/s12161-016-0409-6

    Article  Google Scholar 

  26. Ferrone V, Cotellese R, Carlucci M, Di Marco L, Carlucci G (2018) Air assisted dispersive liquid-liquid microextraction with solidification of the floating organic droplets (AA-DLLME-SFO) and UHPLC-PDA method: application to antibiotics analysis in human plasma of hospital acquired pneumonia patients. J Pharm Biomed Anal 151:266–273. https://doi.org/10.1016/j.jpba.2017.12.039

    Article  CAS  PubMed  Google Scholar 

  27. Teglia CM, Gonzalo L, Culzoni MJ, Goicoechea HC (2019) Determination of six veterinary pharmaceuticals in egg by liquid chromatography: chemometric optimization of a novel air assisted-dispersive liquid-liquid microextraction by solid floating organic drop. Food Chem 273:194–202. https://doi.org/10.1016/j.foodchem.2017.08.034

    Article  CAS  PubMed  Google Scholar 

  28. Yu H, Tao Y, Chen D, Wang Y, Yuan Z (2011) Development of an HPLC–UV method for the simultaneous determination of tetracyclines in muscle and liver of porcine, chicken and bovine with accelerated solvent extraction. Food Chem 124:1131–1138. https://doi.org/10.1016/j.foodchem.2010.07.024

    Article  CAS  Google Scholar 

  29. Song J, Zhang ZH, Zhang YQ, Feng C, Wang GN, Wang JP (2014) Ionic liquid dispersive liquid–liquid microextraction combined with high performance liquid chromatography for determination of tetracycline drugs in eggs. Anal Methods 6:6459–6466. https://doi.org/10.1039/c4ay01079e

    Article  CAS  Google Scholar 

  30. You X, Chen X, Liu F, Hou F, Li Y (2018) Ionic liquid-based air-assisted liquid-liquid microextraction followed by high performance liquid chromatography for the determination of five fungicides in juice samples. Food Chem 239:354–359. https://doi.org/10.1016/j.foodchem.2017.06.07

    Article  CAS  PubMed  Google Scholar 

  31. Zhou Q, Jin Z, Li J, Wang B, Wei X, Chen J (2018) A novel air-assisted liquid-liquid microextraction based on in-situ phase separation for the HPLC determination of bisphenols migration from disposable lunch boxes to contacting water. Talanta 189:116–121. https://doi.org/10.1016/j.talanta.2018.06.072

    Article  CAS  PubMed  Google Scholar 

  32. Tu C, Dai Y, Xu K, Qi M, Wang W, Wu L, Wang A (2019) Determination of tetracycline in water and honey by iron(II, III)/Aptamer-based magnetic solid-phase extraction with high-performance liquid chromatography analysis. Anal Lett 52:1653–1669. https://doi.org/10.1080/00032719.2018.1560458

    Article  CAS  Google Scholar 

  33. Gissawong N, Boonchiangma S, Mukdasai S, Srijaranai S (2019) Vesicular supramolecular solvent-based microextraction followed by high performance liquid chromatographic analysis of tetracyclines. Talanta 200:203–211. https://doi.org/10.1016/j.talanta.2019.03.049

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was financially supported by the Fundamental Research Funds for the Central Universities of China (No. 2012017yjsy206).

Author information

Authors and Affiliations

  1. School of Public Health, Southwest Medical University, Luzhou, 646000, China

    Yi Yang

  2. West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China

    Yi Yang, Shuo Yin, Ling Wu, Yongxin Li & Chengjun Sun

Authors
  1. Yi Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuo Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ling Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yongxin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chengjun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chengjun Sun.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Compliance with ethics requirements

This study does not contain human or animal subjects.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Yin, S., Wu, L. et al. Application of ionic liquid-based air-assisted dispersive liquid–liquid microextraction combined with high-performance liquid chromatography for the determination of six tetracyclines in honey. Eur Food Res Technol 247, 2777–2785 (2021). https://doi.org/10.1007/s00217-021-03838-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-021-03838-3

Keywords

Search

Navigation